Localization of Leptin Receptor mRNA Splice Variants in Murine Peripheral Tissues by RT-PCR andin SituHybridization

Hoggard, Nigel; Mercer, Julian G.; Rayner, D V; Moar, Kim-Marie; Trayhurn, Paul; Williams, Lynda M.

doi:10.1006/bbrc.1997.6245

Cited by 278 publications

(208 citation statements)

References 14 publications

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“…The mRNA coding for the long form of the leptin receptor (Ob-Rb) has been found in various brain structures, including nuclei involved in the regulation of energy balance such as the VMH, the PVN, and the ARC [26,41].…”

Section: Introductionmentioning

confidence: 99%

Regulation of corticotropin-releasing factor and urocortin 2/3 mRNA by leptin in hypothalamic N39 cells

et al. 2013

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Corticotropin-releasing factor (CRF) activates the pituitary-adrenal axis during stress, and shows anorectic effects via CRF type 1 receptors in the hypothalamus.Both urocortin (Ucn) 2 and Ucn3 also act as anorectic neuropeptides via CRF type 2 receptors. Leptin, a product of the obesity gene secreted mainly from adipose tissue, reduces food intake and increases energy expenditure. A possible interaction between leptin and CRF/Ucns has been suggested, as leptin can regulate expression and activation of CRF and Ucns in the hypothalamus. This study aimed to explore the possible function of leptin in the hypothalamus, and its effects in regulating CRF and Ucns. The study identified mRNA expression of the leptin receptor (Ob-R) and its subtypes, CRF, and Ucn2/3 in mouse hypothalamic N39 cells. Leptin stimulated signal transducer and activators of transcription type 3 (STAT3) phosphorylation, directly increased the mRNA levels of both CRF and Ucn2/3 in hypothalamic cells, and increased Ob-Rb mRNA levels. A Janus kinase inhibitor inhibited the leptin-mediated increase in STAT3 phosphorylation, and then the increases in CRF and Ucn2/3 mRNA levels. Leptin may contribute to a stress response or anorectic effect via the regulation 2 of CRF and Ucn2/3 in the hypothalamus.

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Section: Introductionmentioning

confidence: 99%

Regulation of corticotropin-releasing factor and urocortin 2/3 mRNA by leptin in hypothalamic N39 cells

et al. 2013

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“…There are at least six splice variants (a-f) of the Ob-R (14). The Ob-Ra variant, possessing a truncated intracellular domain, was the form originally cloned and it has been postulated that it serves to transport leptin across the blood-brain barrier (15).…”

Section: The Leptin Receptormentioning

confidence: 99%

“…Analysis of the expression pattern of the two forms (long and short) of leptin receptors by Northern blot, in situ hybridization and quantitative PCR showed that the receptors are expressed in most tissues (brain, testes, ovary, kidney, spleen, intestines, brown and white adipose tissues, heart, lung, liver, skeletal muscle, and adrenals (14,18)), with the long form being prevalent in the hypothalamus (18), in addition to also being found in the medulla of the adrenal, the inner zone of the medulla of the kidney (16,18), pancreatic islets (19), and fat tissue (18).…”

Section: The Leptin Receptormentioning

confidence: 99%

“…Skeletal muscle and adipose tissue are the most important sites of postprandial glucose disposal, and in these tissues the stimulation of glucose uptake, glycogen synthesis and lipid synthesis are the main effects of insulin. The leptin receptor has been identified in muscle and in adipose tissue (14,18), a fact that makes these tissues possible target sites for the action of leptin.…”

Section: Leptin and The Insulin-sensitive Tissuesmentioning

confidence: 99%

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Pivotal role of leptin in insulin effects

Ceddia

William

Lima

et al. 1998

Braz J Med Biol Res

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The OB protein, also known as leptin, is secreted by adipose tissue, circulates in the blood, probably bound to a family of binding proteins, and acts on central neural networks regulating ingestive behavior and energy balance. The two forms of leptin receptors (long and short forms) have been identified in various peripheral tissues, a fact that makes them possible target sites for a direct action of leptin. It has been shown that the OB protein interferes with insulin secretion from pancreatic islets, reduces insulin-stimulated glucose transport in adipocytes, and increases glucose transport, glycogen synthesis and fatty acid oxidation in skeletal muscle. Under normoglycemic and normoinsulinemic conditions, leptin seems to shift the flux of metabolites from adipose tissue to skeletal muscle. This may function as a peripheral mechanism that helps control body weight and prevents obesity. Data that substantiate this hypothesis are presented in this review.

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“…2 A leptin receptor has a wide anatomic distribution in the mouse (including in the spleen, adrenal, liver, testes, and lung). 3 Pertinently, in addition to its metabolic role leptin has numerous other effects, including mitogenesis in several tumor and non-tumor cell lines. 4 Given these findings, we sought to determine the expression of leptin and its receptor in melanoma and nevi.…”

mentioning

confidence: 99%

Lack of maturation with anti-leptin receptor antibody in melanoma but not in nevi

et al. 2009

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We have previously shown thryotropin-releasing hormone expression in nevi and melanoma. Thryotropinreleasing hormone regulation by leptin has been shown in the hypothalamus. The present study was therefore undertaken to evaluate leptin and leptin receptor in nevi and melanoma. Leptin receptor expression as assessed using an anti-leptin receptor antibody showed uniform expression throughout the lesion in 14 of 17 melanomas; 3 melanomas lacked leptin receptor immunoreactivity. In contrast, out of 15 nevi, 10 showed weak to moderate leptin receptor immunoreactivity, with positivity present only in the superficial dermal component. Thus, maturation was present in nevi but not in melanoma with the anti-leptin receptor antibody (Po0.0001). Anti-leptin antibody, in contrast, did not show a significant difference in maturation between nevi and melanoma. We also compared leptin receptor in Spitz nevi and melanoma, as the two can sometimes be difficult to distinguish. Spitz nevi showed moderate to strong immunopositivity. Of 19 Spitz nevi, 7 showed lack of maturation, a finding statistically significant from both melanoma and nevi. Our results suggest a role for leptin receptor in melanoma, and we show for the first time that melanomas show more intense immunoreactivity as compared to nevi (but not Spitz nevi) and that maturation with anti-leptin receptor antibody may be a diagnostically useful tool in distinguishing melanomas, especially nevoid ones, from nevi in difficult cases.

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Localization of Leptin Receptor mRNA Splice Variants in Murine Peripheral Tissues by RT-PCR andin SituHybridization

Cited by 278 publications

References 14 publications

Regulation of corticotropin-releasing factor and urocortin 2/3 mRNA by leptin in hypothalamic N39 cells

Regulation of corticotropin-releasing factor and urocortin 2/3 mRNA by leptin in hypothalamic N39 cells

Pivotal role of leptin in insulin effects

Lack of maturation with anti-leptin receptor antibody in melanoma but not in nevi

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