Localization of interleukin-18 and its receptor in somatotrophs of the bovine anterior pituitary gland

Nagai, Yoshinori; Nochi, Tomonori; Watanabe, Kouichi; Watanabe, Keisuke; Aso, Hisashi; Kitazawa, Haruki; Matsuzaki, Masatoshi; Ohwada, Shyuichi; Yamaguchi, Takahiro

doi:10.1007/s00441-005-0016-0

Cited by 19 publications

(15 citation statements)

References 34 publications

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“…Total RNA was extracted from monocytes and monocytederived macrophages by using TRIzol reagent (Gibco BRL, Grand Island, NY) according to the manufacturer's protocol as previously described (9,10). The first strand cDNAs were synthesized using the Superscript III reverse transcriptase (RT; Invitrogen, Carlsbad, CA) with the oligo (dT) primer.…”

Section: Rna Extraction and Semi-quantitative Rt-pcrmentioning

confidence: 99%

Differential Responses Between Monocytes and Monocyte-Derived Macrophages for Lipopolysaccharide Stimulation of Calves

2009

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In this experiment Toll-like receptor expression pattern in monocytes and monocyte-derived macrophages by lipopolysaccharide (LPS) stimulation was examined. Jugular venous blood was collected from four Japanese calves, and the peripheral blood mononuclear cells (PBMCs) were isolated. The cells were directly used for collecting monocytes by magnetic cell sorting or cultured for 7 days to collect monocyte-derived macrophages in Repcell. Then we analyzed the mRNA expression pattern of TLRs and cytokines in monocytes and monocyte-derived macrophages after LPS stimulation for 24 h. LPS stimulation of both monocytes and monocyte-derived macrophages resulted in an increase in the levels of mRNA transcripts for TNF-, IL-6 and IL-8. Moreover, TNF- and IL-6 mRNA expressions were significantly augmented by LPS stimulation in monocyte-derived macrophages. TLRs mRNA expressions were unchanged after LPS stimulation of monocytes, while TLRs mRNA expressions in monocyte-derived macrophages were complicated. TLR1, 3, 5, 8 and 10 were significantly decreased after LPS stimulation and there were no differences in the mRNA expressions of TLR2, 4, 6 and 7 between the groups of control and LPS stimulation. Besides, no expression of TLR9 was found. As antigen presenting cells, monocytes and monocyte-derived macrophages respond differently to LPS, so they may have different functions in the innate immune system. Cellular & Molecular Immunology. 2009;6(3):223-229.

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Section: Rna Extraction and Semi-quantitative Rt-pcrmentioning

confidence: 99%

Differential Responses Between Monocytes and Monocyte-Derived Macrophages for Lipopolysaccharide Stimulation of Calves

2009

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“…In addition, bovine somatotropes have been shown to produce IL-18 and IL-18Rα was co-localized with IL-18, or growth hormone, suggesting the possibility that IL-18 acts on somatotropes through the autocrine pathway [80]. However, IL-18 seems to also act at the hypothalamic level.…”

Section: Introductionmentioning

confidence: 99%

Interleukin 18 in the CNS

Alboni

Cervia

Sugama

et al. 2010

J Neuroinflammation

250

201

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Interleukin (IL)-18 is a cytokine isolated as an important modulator of immune responses and subsequently shown to be pleiotropic. IL-18 and its receptors are expressed in the central nervous system (CNS) where they participate in neuroinflammatory/neurodegenerative processes but also influence homeostasis and behavior. Work on IL-18 null mice, the localization of the IL-18 receptor complex in neurons and the neuronal expression of decoy isoforms of the receptor subunits are beginning to reveal the complexity and the significance of the IL-18 system in the CNS. This review summarizes current knowledge on the central role of IL-18 in health and disease.

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“…The expression of IL-2, IL-4, IL-8, IL-11 and IL-18, has been reported [41][42][43][44][45][46][47][48], although IL-1b and IL-6 seem to play a more important functional role (see Section 1). Nevertheless, there are hardly any references to the expression of these two interleukins during the development of the adenohypophysis.…”

Section: Discussionmentioning

confidence: 99%

Prenatal expression of interleukin 1β and interleukin 6 in the rat pituitary gland

et al. 2008

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a b s t r a c tIt is known that interleukin 1b (IL-1b) and interleukin 6 (IL-6) are expressed post-natally in normal and tumoral cells in the anterior pituitary, and that they play a role in both the liberation of different hormones and in the growth, proliferation and tumor formation of the pituitary gland. However, their expression and role during embryonic and fetal development remain unknown. We have performed an immunocytochemistry study of prenatal expression and distribution of IL-1b and IL-6 in isolated embryonic rat Rathke's pouch prior to birth, more specifically between 13.5 and 19.5 days p.c. Western-blot analysis carried out on 19.5-day p.c. embryos showed positive immunolabelling for IL-1b and IL-6. These interleukins were initially expressed simultaneously in the rostral and ventral portions of Rathke's pouch in 15.5-day p.c. embryos, and this expression progressed caudodorsally in later developmental stages, extending to most of the hypophysis before birth. The number of cells expressing these interleukins increased throughout this period: 48.22% of anterior pituitary cells expressed IL-6 in 19.5-day embryos, whilst IL-1b was positive in 39.8% of the cells. Moreover, we have demonstrated that some adenohypophyseal cells co-express both interleukins. Such findings represent the first step towards an understanding of the physiological role of these interleukins in anterior pituitary development.

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Localization of interleukin-18 and its receptor in somatotrophs of the bovine anterior pituitary gland

Cited by 19 publications

References 34 publications

Differential Responses Between Monocytes and Monocyte-Derived Macrophages for Lipopolysaccharide Stimulation of Calves

Differential Responses Between Monocytes and Monocyte-Derived Macrophages for Lipopolysaccharide Stimulation of Calves

Interleukin 18 in the CNS

Prenatal expression of interleukin 1β and interleukin 6 in the rat pituitary gland

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