Localization of immunoreactive endothelin in the neuroendocrine cells of fish gill

Zaccone, Giacomo; Mauceri, Angela; Fasulo, Salvatore; Ainis, L.; Cascio, Patrizia Lo; Ricca, M. B.

doi:10.1016/s0143-4179(96)90055-4

Cited by 36 publications

(19 citation statements)

References 20 publications

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“…Proendothelin-1 immunoreactivity was seen in epithelial cells adjacent to cells immunoreactive for NKA (Fig.·6A,C), and these cells share similar morphology to neuroendocrine cells (NECs) described in fishes (Zaccone et al, 1992;Goniakowska-Witalinska et al, 1995;Zaccone et al, 1996;Mauceri et al, 1999). This immunolocalization matches the killifish EDN1 mRNA localization shown in Fig.·5.…”

Section: Immunohistochemistrysupporting

confidence: 79%

“…Proendothelin immunoreactivity was also found on pillar cells, which is in agreement with our in situ hybridization findings. ProEDN was immunolocalized to gill neuroendocrine cells (NECs) of the eel (Conger congo), catfish (Heteropneustes fossilis) and dogfish (Scyliorhfnus canicula), and the morphology of these cells matches that of the proEDN immunoreactive epithelial cells in the killifish gill (Zaccone et al, 1996).…”

Section: Gill Expression Of Edn1 Mrna and Proedn1 Proteinmentioning

confidence: 86%

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Endothelin and endothelin converting enzyme-1 in the fish gill:evolutionary and physiological perspectives

Hyndman

Evans

2007

Journal of Experimental Biology

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SUMMARY In euryhaline fishes like the killifish (Fundulus heteroclitus)that experience daily fluctuations in environmental salinity, endothelin 1(EDN1) may be an important regulator molecule necessary to maintain ion homeostasis. The purpose of this study was to determine if EDN1 and the endothelin converting enzyme (ECE1; the enzyme necessary for cleaving the precursor proendothelin-1 to EDN1) are present in the killifish, to determine if environmental salinity regulates their expression, and to examine the phylogenetic relationships among the EDNs and among the ECEs. We sequenced killifish gill cDNA for two EDN1 orthologues, EDN1A and EDN1B, and also sequenced a portion of ECE1 cDNA. EDN1A and ECE1 mRNA are expressed ubiquitously in the killifish while EDN1B mRNA has little expression in the killifish opercular epithelium or gill. Using in situ hybridization and immunohistochemistry, EDN1 was localized to large round cells adjacent to the mitochondrion-rich cells of the killifish gill, and to lamellar pillar cells. In the gill, EDN1A and EDN1B mRNA levels did not differ with acute (<24 h) or chronic (30 days) acclimation to seawater (SW); however, EDN1B levels increased threefold post SW to freshwater (FW) transfer,and ECE1 mRNA levels significantly increased twofold over this period. ECE1 mRNA levels also increased sixfold over 24 h post FW to SW transfer. Chronic exposure to SW or FW had little effect on ECE1mRNA levels. Based upon our cellular localization studies, we modeled EDN1 expression in the fish gill and conclude that it is positioned to act as a paracrine regulator of gill functions in euryhaline fishes. It also may function as an autocrine on pillar cells, where it is hypothesized to regulate local blood flow in the lamellae. From our phylogenetic analyses, ECE is predicted to have an ancient origin and may be a generalist endoprotease in non-vertebrate organisms, while EDNs are vertebrate-specific peptides and may be key characters in vertebrate evolution.

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Section: Immunohistochemistrysupporting

confidence: 79%

Section: Gill Expression Of Edn1 Mrna and Proedn1 Proteinmentioning

confidence: 86%

Endothelin and endothelin converting enzyme-1 in the fish gill:evolutionary and physiological perspectives

Hyndman

Evans

2007

Journal of Experimental Biology

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“…Thus species differences may exist in the specific distribution of ET receptors in the fish gills. The site of ET synthesis appears to be vascular endothelial and neuroepithelial cells in the filamental epithelium in a variety of fish species including teleosts and elasmobranchs, (852,854). In Zaccone's studies, big ET (ET precursor) immunoreactivity colocalized with serotonin and neuropoeptide immunoreactivity in some neuroepithelial cells.…”

Section: Etmentioning

confidence: 99%

The Multifunctional Fish Gill: Dominant Site of Gas Exchange, Osmoregulation, Acid-Base Regulation, and Excretion of Nitrogenous Waste

Evans¹,

Piermarini²,

Choe³

2005

Physiological Reviews

2,289

1,788

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The fish gill is a multipurpose organ that, in addition to providing for aquatic gas exchange, plays dominant roles in osmotic and ionic regulation, acid-base regulation, and excretion of nitrogenous wastes. Thus, despite the fact that all fish groups have functional kidneys, the gill epithelium is the site of many processes that are mediated by renal epithelia in terrestrial vertebrates. Indeed, many of the pathways that mediate these processes in mammalian renal epithelial are expressed in the gill, and many of the extrinsic and intrinsic modulators of these processes are also found in fish endocrine tissues and the gill itself. The basic patterns of gill physiology were outlined over a half century ago, but modern immunological and molecular techniques are bringing new insights into this complicated system. Nevertheless, substantial questions about the evolution of these mechanisms and control remain.

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“…For immunohistochemical staining, sections were processed for the indirect immunoperoxidase method, as previously reported (Zaccone et al, 1996;Mauceri et al, 1999) using heterologous antisera. Details of the antisera are presented in Table I.…”

Section: Immunohistochemistrymentioning

confidence: 99%

Neurochemical features of the innervation of respiratory organs in some air‐breathing fishes

Mauceri¹,

Fasulo

Minniti

et al. 2005

Italian Journal of Zoology

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A number of studies has been performed in recent years regarding the structure of the autonomic innervation of the gill and various organs of fishes, but only limited information exists on the role of the autonomic nervous system in air-breathing organs. The mechanisms for physiologically integrating gill and air-breathing organs are also not fully understood. The presence of neuropeptides and nNOS in gill autonomie nerves has been investigated in recent years by several Authors. Immunohistochemical localization of neuropeptides and nNOS in the autonomie nerves in the vasculatures and smooth muscle of air-breathing organs is reported for the first time. The function of NO in these tissues also remains to be elucidated especially, when the bulk of neurotransmission is attributable to cholinergic and adrenergic mechanisms. A challenge for future years is to understand the role of the autonomie nervous systems and the vasoactive substances in the in-series and in-parallel vascular connections between systemic gill and air-breathing organs.

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Localization of immunoreactive endothelin in the neuroendocrine cells of fish gill

Cited by 36 publications

References 20 publications

Endothelin and endothelin converting enzyme-1 in the fish gill:evolutionary and physiological perspectives

Endothelin and endothelin converting enzyme-1 in the fish gill:evolutionary and physiological perspectives

The Multifunctional Fish Gill: Dominant Site of Gas Exchange, Osmoregulation, Acid-Base Regulation, and Excretion of Nitrogenous Waste

Neurochemical features of the innervation of respiratory organs in some air‐breathing fishes

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