Localization of <i>Xcat</i>-2 RNA, a putative germ plasm component, to the mitochondrial cloud in <i>Xenopus</i> stage I oocytes

Yi, Zili; King, Mary Lou

doi:10.1242/dev.122.9.2947

Cited by 101 publications

(3 citation statements)

References 36 publications

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“…We divided The results reveal that nearly all Xenopus germline RNAs are I-S transcripts. These include ddx25 32 , nanos1 33 , and dnd1 34 , and genes recently found to be important for PGC development 35 . Many germline regulators form a protein-protein interaction network (Fig 3C).…”

Section: Soluble-to-insoluble Phase Transition Of Maternal Rna During...mentioning

confidence: 99%

Phase transition of maternal RNAs during vertebrate oocyte-to-embryo transition

Hwang,

Chen,

et al. 2023

Preprint

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The oocyte-to-embryo transition (OET) is regulated by maternal products stored in the oocyte cytoplasm, independent of transcription. How maternal products are precisely remodeled to dictate the OET remains an open question. In this work, we discover the dynamic phase transition of maternal RNAs duringXenopusOET. We have identified 863 maternal transcripts that transition from a soluble state to a detergent-insoluble one after oocyte maturation. These RNAs are enriched in the animal hemisphere and many of them encode key cell cycle regulators. In contrast, 165 transcripts, including nearly allXenopusgermline RNAs and some vegetally localized somatic RNAs, undergo an insoluble-to-soluble phase transition. This phenomenon is conserved in zebrafish. Our results demonstrate that the phase transition of germline RNAs influences their susceptibility to RNA degradation machinery and is mediated by the remodeling of germ plasm. This work thus uncovers novel remodeling mechanisms that act on RNAs to regulate vertebrate OET.

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Section: Soluble-to-insoluble Phase Transition Of Maternal Rna During...mentioning

confidence: 99%

Phase transition of maternal RNAs during vertebrate oocyte-to-embryo transition

Hwang,

Chen,

et al. 2023

Preprint

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“…Indeed, a similar concept is also seen during Xenopus and Drosophila germ plasm development. Here, different cis-acting elements target mRNAs at different stages of the localization process [126][127][128][129].…”

Section: The Axon-dendrite Paradoxmentioning

confidence: 99%

Molecular mechanisms behind mRNA localization in axons

2020

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Messenger RNA (mRNA) localization allows spatiotemporal regulation of the proteome at the subcellular level. This is observed in the axons of neurons, where mRNA localization is involved in regulating neuronal development and function by orchestrating rapid adaptive responses to extracellular cues and the maintenance of axonal homeostasis through local translation. Here, we provide an overview of the key findings that have broadened our knowledge regarding how specific mRNAs are trafficked and localize to axons. In particular, we review transcriptomic studies investigating mRNA content in axons and the molecular principles underpinning how these mRNAs arrived there, including cis-acting mRNA sequences and trans-acting proteins playing a role. Further, we discuss evidence that links defective axonal mRNA localization and pathological outcomes.

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“…Previous studies have shown the presence of three distinct pathways for vegetal RNA localization. The first one is called the early pathway (also known as METRO) and is used mainly for the localization of germ plasm determinants such as nanos1 ( Forristall et al, 1995 ; Kloc and Etkin, 1995 ; Zhou and King, 1996 ), dazl ( Houston et al, 1998 ) and ddx25 ( MacArthur et al, 2000 ) . During early oogenesis, mRNAs diffuse from the nucleus to be entrapped by the mitochondrial cloud (in fish, called Balbiani body).…”

Section: Introductionmentioning

confidence: 99%

RNA localization during early development of the axolotl

Šimková,

Naraine,

Vintr

et al. 2023

Front. Cell Dev. Biol.

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The asymmetric localization of biomolecules is critical for body plan development. One of the most popular model organisms for early embryogenesis studies is Xenopus laevis but there is a lack of information in other animal species. Here, we compared the early development of two amphibian species—the frog X. laevis and the axolotl Ambystoma mexicanum. This study aimed to identify asymmetrically localized RNAs along the animal-vegetal axis during the early development of A. mexicanum. For that purpose, we performed spatial transcriptome-wide analysis at low resolution, which revealed dynamic changes along the animal-vegetal axis classified into the following categories: profile alteration, de novo synthesis and degradation. Surprisingly, our results showed that many of the vegetally localized genes, which are important for germ cell development, are degraded during early development. Furthermore, we assessed the motif presence in UTRs of degraded mRNAs and revealed the enrichment of several motifs in RNAs of germ cell markers. Our results suggest novel reorganization of the transcriptome during embryogenesis of A. mexicanum to converge to the similar developmental pattern as the X. laevis.

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Localization of Xcat-2 RNA, a putative germ plasm component, to the mitochondrial cloud in Xenopus stage I oocytes

Cited by 101 publications

References 36 publications

Phase transition of maternal RNAs during vertebrate oocyte-to-embryo transition

Phase transition of maternal RNAs during vertebrate oocyte-to-embryo transition

Molecular mechanisms behind mRNA localization in axons

RNA localization during early development of the axolotl

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