Localization of high-affinity GABA uptake and GABA content in the rat duodenum during development

Gilon, Patrick; Reusens-Billen, B; Remacle, Claude; Varebeke, P. Janssens De; Pauwels, Greet; Hoet, J. J.

doi:10.1007/bf00217331

Cited by 39 publications

(18 citation statements)

References 27 publications

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“…The presence of GABA-immunoreactivity and GABA uptake in endocrine cells of the rat stomach is in good agreement with other studies, revealing immunostaining and autoradiographic labelling in endocrine cells of the duodenum and epithelial cells of the gastro-intestinal tract (Gilon et al 1987b(Gilon et al , 1988Jessen et al 1988 ;Davanger et al 1989). The majority of these cells in the gastric epithelium lack any contact with the lumen of the glands.…”

Section: Immunocytochemical and Autoradiographic Localization Of Gabasupporting

confidence: 91%

Immunocytochemical and autoradiographic studies of the endocrine cells interacting with GABA in the rat stomach

et al. 1990

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There are now increasing evidences suggesting that GABA is able of direct interaction with certain endocrine cells. In the present study, highly specific anti-GABA-glutaraldehyde antibodies and 3H-GABA uptake were used at the light and electron microscope levels to investigate the occurrence of cells containing endogenous GABA or taking up exogenous GABA in the mucosal antrum and corpus of the rat stomach. Only certain endocrine cell types of both regions were immunostained or grain-labelled. However, the morphology of their secretory granules did not allow to identify the nature of their hormone with certainty but suggested that somatostatin-like cells could interact with GABA. The combination of gastrin and somatostatin immunodetection with 3H-GABA uptake autoradiography at the light microscope level, revealed that a subpopulation of somatostatin-like cells and other still unidentified endocrine cells are able to take up GABA, while the gastrin-like cells are not. These results rei... Summary. There are now increasing evidences suggesting that GABA is able of direct interaction with certain endocrine cells. In the present study, highly specific anti-GABAglutaraldehyde antibodies and aH-GABA uptake were used at the light and electron microscope levels to investigate the occurrence of cells containing endogenous GABA or taking up exogenous GABA in the mucosal antrum and corpus of the rat stomach. Only certain endocrine cell types of both regions were immunostained or grain-labelled. However, the morphology of their secretory granules did not allow to identify the nature of their hormone with certainty but suggested that somatostatin-like cells could interact with GABA. The combination of gastrin and somatostatin immunodetection with aH-GABA uptake autoradiography at the light microscope level, revealed that a subpopulation of somatostatin-like cells and other still unidentified endocrine cells are able to take up GABA, while the gastrin-like cells are not. These results reinforce the hypothesis that certain endocrine cell types of the diffuse endocrine system of the digestive tract are able to directly interact with GABA.

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Section: Immunocytochemical and Autoradiographic Localization Of Gabasupporting

confidence: 91%

Immunocytochemical and autoradiographic studies of the endocrine cells interacting with GABA in the rat stomach

et al. 1990

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“…Furthermore, many nonneuronal cells take up GABA by a sodium-dependent, carrier-mediated process, amongst them glial [14], pancreatic [15] and adrenal chromaftln cells [8,16]. In the central nervous system, GABA is stored in SSV and released by exocytosis from GABAergic neurons acting as an inhibitory transmitter via specific receptors (for a review see [17]).…”

Section: Discussionmentioning

confidence: 99%

The amphicrine pancreatic cell line, AR42J, secretes GABA and amylase by separate regulated pathways

Ahnert‐Hilger

Wiedenmann

1992

FEBS Letters

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Treatment of AR42J cells wi~h dexamethasone leads to an enhanced formation of amylase-containing granules and facilitates their regulated secretion, Besides tile exocrine properties, AR4~ cells possess a specific uptake system for [~H]GABA. The stored GABA can be released upon pota.~sium depolarisation in a Ca-"-dependent manner. After treatment with dexamethasoae, potassium depolarisation fails to release GABA, but instead causes a Ca"*.dependent secretion of amylase. Since vesicles similar to small synaptic vesicles of neurons have been identified in AR4~ cells, we suggest that the regulated GABA release is mediated by this vesicle type. It is tentatively speculated that other epithelial ¢¢11s, which also contain small synaptic vesicles and amino acid neurotransmitters, ma~, release them in a similar fashion.

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“…Hepatic venous glu cose concentrations increased following por tal venous infusions of GABA in rats but the increase never exceeded 15% of baseline val ues [56], The possibility that GABA might play a role in regulating hepatic regenerative activity is intriguing for a number of reasons. Firstly, it had previously been shown that GABA con centrations increase as the development of tis sues in utero approach maturation [58][59][60][61], This had been interpreted as indicating that GABA was responsible for or at least contrib uted to the suppression of rapid growth by these tissues. Secondly, exogenous GABA had been reported to completely inhibit the growth of murine squamous cell carcinoma 50 Minuk GABA and the Liver …”

Section: Gaba Receptors In the Livermentioning

confidence: 99%

Gamma-Aminobutyric Acid and the Liver

Minuk

1993

Dig Dis

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Gamma-aminobutyric acid (GABA) is a potent amino acid neurotransmitter with various physiologic effects throughout the body. Over the past 40 years it has become evident that the mammalian liver contains high concentrations of GABA which are carefully regulated by a series of hepatic metabolic pathways and a specific sodium-dependent active transport system. It is also clear that the liver possesses sodium-independent, bicuculline-sensitive GABA_A receptor sites which when innervated cause marked hyperpolarization of the hepatocyte transmembrane potential. Recent data suggest that extensive hepatic injury alters GABA homeostatic mechanisms which in turn may contribute to the pathogenesis of hepatic encephalopathy and systemic hypotension. There is also reason to believe that increased GABAergic activity may inhibit hepatic regeneration following partial hepatectomy in rats.

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Localization of high-affinity GABA uptake and GABA content in the rat duodenum during development

Cited by 39 publications

References 27 publications

Immunocytochemical and autoradiographic studies of the endocrine cells interacting with GABA in the rat stomach

Immunocytochemical and autoradiographic studies of the endocrine cells interacting with GABA in the rat stomach

The amphicrine pancreatic cell line, AR42J, secretes GABA and amylase by separate regulated pathways

Gamma-Aminobutyric Acid and the Liver

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