Localization of fruit tree viruses by immuno-tissue printing in infected shoots of Malus sp. and Prunus sp.

Knapp, E.; Machado, Artur da Câmara; Pühringer, Helene; Wang, Q.; Hanzer, V.; Weiß, H.; Weiss, Batia; Katinger, Hermann; Machado, M. Laimer da Câmara

doi:10.1016/0166-0934(95)00033-q

Cited by 47 publications

(26 citation statements)

References 20 publications

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“…LP-free SAMs are suitable for use in production of disease-free plants that would otherwise be difficult to achieve by conventional methods (Laimer 2003). Some types of virus, such as apple stem grooving virus, are transmitted close to the SAM and, in this case, it is important to culture only a small portion of the dome (Knapp et al 1995;Laimer 2003). Cabbage root tips were observed to be suitable for rescuing the LP-free SAMs of other plant species such as Fragaria or Dianthus (unpublished data) Growth characteristics of CSVd-free chrysanthemum plants…”

Section: Resultsmentioning

confidence: 99%

“…However, if a sensitive method were used for detection, CSVd might be detected in these "viroid-free" plants. Knapp et al (1995) observed that apple stem grooving virus exists even in the youngest leaf primordium of Malus and Prunus. In addition, potato spindle tuber viroid (PSTVd) was reported not to exist in the apical meristem dome of potato (Zhu et al 2001).…”

Section: Introductionmentioning

confidence: 96%

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Elimination of chrysanthemum stunt viroid from an infected chrysanthemum cultivar by shoot regeneration from a leaf primordium-free shoot apical meristem dome attached to a root tip

et al. 2004

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In this research we eliminated chrysanthemum stunt viroid (CSVd) from a highly infected chrysanthemum cultivar using a newly established method. 'Piato' is one of the most difficult cultivars in which to obtain CSVd-free plants by conventional methods. Leaf primordium-free shoot apical meristems (LP-free SAMs) of 'Piato' plants were dissected and attached to CSVd-free chrysanthemum or cabbage root tips. As shown by nested-PCR, CSVd was not detected in some shoots regenerated on both types of root tip. The production rates of CSVd-free plants using chrysanthemum and cabbage root tips were 14% and 3%, respectively. Regeneration of plants from LP-free SAMs of chrysanthemum plants by attaching these SAMs to root tips is an efficient method of generating CSVd-free chrysanthemum plants.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 96%

Elimination of chrysanthemum stunt viroid from an infected chrysanthemum cultivar by shoot regeneration from a leaf primordium-free shoot apical meristem dome attached to a root tip

et al. 2004

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“…Knapp et al (1995) observed that apple stem grooving virus exists even in the youngest leaf primordium using Malus and Prunus. These two observations indicate the importance of LP-free SAM as an explant for the production of pathogen-free plant that are difficult to produce by shoot regeneration from a meristem with leaf primordia.…”

Section: Resultsmentioning

confidence: 96%

Rescue of shoot apical meristems of chrysanthemum by culturing on root tips

et al. 2004

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A new method to regenerate plants from leaf primordia-free shoot apical meristem domes (LP-free SAMs) was developed by establishing the meristem dome on the cut surface of root tips. Ten days after culture, the viable rate of LP-free SAMs of chrysanthemum 'Piato' attached to chrysanthemum root tips was >40%. Shoot regeneration was not observed from LP-free SAMs without the root tips. When LP-free SAMs of chrysanthemum were transferred to root tips of either petunia, cabbage, or carnation, the highest shoot regeneration rate was observed with cabbage root tips. Microscopic observation documented that the LP-free SAM temporarily adhered to the cut surface of the root tip of cabbage.

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“…Low levels of virus can be a virus specific trait, as is the case with ASGV, which occurs extrernly limited and highly localized in host tissues (Fuchs et aI., 1988, Knapp et at, 1995c. However there remain the limitations of current ELISA-based serological tests which might be not sensitive enough to detect very low levels of virus.…”

Section: Discussionmentioning

confidence: 99%