Localization of fluorescent substances in the cellular slime moldDictyostelium discoideum cells during growth and development

Uchiyama, Saburo; Nagai, S.; Maruyama, Keizo

doi:10.1007/bf02345979

Cited by 5 publications

(4 citation statements)

References 26 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…This is consistent with the results from studies of the spore mass ofD. discoideum as described elsewhere (21). DISCUSSION In this study we observed the localization of fluorescence in amoebae,slugs, and spore masses ofD.…”

Section: Resultssupporting

confidence: 92%

“…It has been reported that D. discoideum cells secrete lumazine (the demination product of pterin), an extracellular fluorescent product that has been linked with their ability to aggregate (19,20). We previously observed fluorescence in the live cells of D. discoideum during their growth and morphogenesis (21). In present study, we examined the localization of fluorescence in the cells and cell mass of D. mucoroides by fluorescence microscopy.…”

mentioning

confidence: 87%

See 1 more Smart Citation

Fluorescence in the Migrating Pseudoplasmodium of the Cellular Slime Mold Dictyostelium mucoroides.

Uchiyama

Nagai

Maruyama

1994

Cell Struct. Funct.

Self Cite

View full text Add to dashboard Cite

ABSTRACT. Fluorescence was observed under light microscope in living cells and cell mass of Dictyostelium mucoroides. The fluorescence was localized in the vacuoles of live vegetative cells. While the cell mass of D. discoideum does not have a stalk during migration period, the cell mass of D. mucoroides has a stalk that forms at the beginning of the migration period. Wewere able to observe a preferential loss of the fluorescent vacuoles from the cells of the stalk and from the stalk-forming cells at the tip region of the slug. Although the fluorescence was also present in the mature spore mass of Z>. mucoroides, the fluorescence was not observed in the spores, but rather in the spaces between the spores within the spore mass. The fluorescent vacuoles in the cells of vegetative stage and of migrating slug stage may be related to the interspore fluorescence in the spore mass. Possible roles of the fluorescent substance(s) in amoebae, slugs and spore masses were discussed.Amoebaeof the cellular slime mold Dictyostelium mucoroides proliferate by binary fission, using bacteria as a food source. After deprivation of bacteria, the homogeneouspopulation of slime mold cells aggregates to form a slug-shaped mass of cells (pseudoplasmodium) on a solid substratum. The cell mass ofD. mucoroides has a stalk that forms at the beginning of the migration period, during which time the cell mass crawls over the surface of the substratum. Eventually the mass of cells generates a fruiting body, which consists of a mass of spores and a supporting cellular stalk. The cells grown in liquid nutrient mediumremain stable for an extended period of time prior to cell lysis (18). This period is referred to as the stationary phase.

show abstract

Section: Resultssupporting

confidence: 92%

mentioning

confidence: 87%

Fluorescence in the Migrating Pseudoplasmodium of the Cellular Slime Mold Dictyostelium mucoroides.

Uchiyama

Nagai

Maruyama

1994

Cell Struct. Funct.

Self Cite

View full text Add to dashboard Cite

show abstract

“…4d). This would correspond to fluorescence substance reported in an early paper (Uchiyama et al 1993). Morphological events; cell fusion, karyogamy and development of thick wall were observed in our crosses of sexually competent cells as in the mating of green algae such as Chlamydomonas reinhardtii (Goodenough andThorner 1989, Harris 1989).…”

Section: Molecular Weights and Isoelectric Pointsmentioning

confidence: 97%

A Maltose-binding-protein-like Peptide Accumulates after Mixing Two Types of Sexually Competent Cells of a Cellular Slime Mold, Dictyostelium discoideum.

et al. 1995

View full text Add to dashboard Cite

A cellular slime mold, Dictyostelium discoideum is an ideal model organism for examining sexual differentiation and mating. Cells on agar plates can obtain sexual competence syn chronously by culturing in liquid medium including food bacteria with shaking. After removing the bacteria, mixing opposite mating type cells of D. discoideum results in beginning of mating. They fuse with each other, forming multinucleate cells. After giant cells are formed, they attract and engulf smaller cells. Giant cells develop into macrocysts. Macrocysts undergo meiosis and at the end of dormancy, their progeny amoebae are released. Though formation of multinuclear cells is unique, serial sexual events such as plasmogany, karyogamy, formation of thick wall, and meiosis are universal in the mating of isogamous organisms. Involvement of Ca2+, cAMP with mating is also the case in fertilizing green algae (Goodenough and Thoner 1983). Analysis of mating mechanism using Dictyostelium cell will reveal common mechanism among various organisms.Dictyostelium discoideum is one of the organisms that glycoproteins at mating are precisely studied as well as sea urchin (Gilbert 1988) 1987 , Ishikawa et al. 1991. These results suggest that sugars play important roles in the gamete differentiation and fusion of mating cells. But there have been a few immunological researches on sugar binding proteins of D. dis coideum, while many papers reported precisely roles of carbohydrates on cell membrane. In bacteria, maltose translocator upon cell membrane is a recognition target for infecting phage lambda (Randall-Hazelbauer and Schwartz 1973), and in human macrophages, mannose receptor mediates phagocytosis of saccharide-coated particles (Warr 1980).We have tried to detect maltose-binding protein in the cell of D. discoideum after beginning of mating using antibody to bacterial maltose-binding protein (MBP).This paper reports accumulation of two MBP-like peptides 24hr after mixing two types of sexually competent cells for the first time. We show morphology of mating cells in DAPI-fluorescence microscopy. We discuss about the nature of isoforms of MBP-like protein, and speculate their potential roles in

show abstract

“…wavelength, 450 nm. Peak labels: (1) neolumazine; (2) neopterin, (3) monalumazhe; (4) monapterin, (5) biolumazine; (6) biopterin; (7) dictyolumazine; (8) dictyopterin.…”

Section: Achiral Hplcunclassified

Chiral lumazines: Preparation, properties, enantiomeric separation

Klein¹,

Tatischeff²,

Tham³

et al. 1994

Chirality

View full text Add to dashboard Cite

Optically active lumazines (biolumazine, dictyolumazine, monalumazine, and neolumazine) are prepared from the corresponding pterins by enzymatic reaction, using pterin deaminase excreted by Dictyostelium discoideum. The fluorescence properties, circular dichroism spectra, and chromatographic behavior of these lumazines are studied. D- and L-enantiomers of biolumazine, dictyolumazine, and monalumazine are separated using a chiral flavoprotein column. This column also separates the enantiomeric pterins of the threo form: monapterin and dictyopterin. However, the column does not separate the enantiomeric pterins of the erythro form: neopterin and biopterin. By coupling a reverse-phase column to the flavoprotein column, the separation of pterins and lumazines in function of their hydrophobicity, as well as the separation of the diastereomers, is achieved. This coupled achiral/chiral high-performance liquid chromatography method enables determination of the stereoconfiguration of natural lumazines by comparison with optically pure compounds. A lumazine derivative, present in the extracellular medium of Dictyostelium discoideum, is identified as D-dictyolumazine, i.e., 6-(D-threo-1,2-dihydroxypropyl)-lumazine.

show abstract

Localization of fluorescent substances in the cellular slime moldDictyostelium discoideum cells during growth and development

Cited by 5 publications

References 26 publications

Fluorescence in the Migrating Pseudoplasmodium of the Cellular Slime Mold Dictyostelium mucoroides.

Fluorescence in the Migrating Pseudoplasmodium of the Cellular Slime Mold Dictyostelium mucoroides.

A Maltose-binding-protein-like Peptide Accumulates after Mixing Two Types of Sexually Competent Cells of a Cellular Slime Mold, Dictyostelium discoideum.

Chiral lumazines: Preparation, properties, enantiomeric separation

Contact Info

Product

Resources

About