Localization of Fluorescent Antibodies to Human Growth Hormone in Human Anterior Pituitary Glands.

Localization of Follicle-Stimulating and Luteinizing Hormones in the Rat Testis Using Immunohistological Tests

“…DISCUSSION The results reported here provide further information on the localization of FSH and LH in the rat testis. The staining obtained with the anti-FSH and anti-LH sera proved specific according to the following criteria (Leznoff, Fishman, Goodfriend, McGarry, Beck & Rose, 1960;Rümke & Ladiges, 1965): (1) staining was achieved exclusively with the antisera and was limited to testicular structures, (2) no staining occurred with non-immune sera or globulins, (3) in the direct test for the demonstra¬ tion of LH, staining was blocked by pretreatment of the sections with unlabelled anti-LH globulins, (4) staining was inhibited when the antisera were first absorbed with the corresponding antigens. Concerning the latter test, it should be noticed that the same gonadotrophic preparations used for immunization were employed as absorbents.…”

The Cytological Localization of Acth in the Human Pituitary*

“…Photomicrographs were made of identical mirror-image fields. Those of the fluorescent sections were printed from the reverse side of the transparency, so that resultant prints were superimposable (9).…”

“…By using antisera to porcine ACTH, comparable amounts of the original antigen, of pure ACTH supplied by Dr. C. H. Li, of human ACTH, and of the beta fraction of Bell inhibited the reaction, while the other Bell fractions showed a weak cross-reaction, as did melanocyte-stimulating hormone (MSH) ( The same specificity controls were employed. These sections were cut and mounted in mirror-image pairs, so that the uppermost surface of each section was the adjacent aspect of the same interface, thus allowing cell-for-cell comparison of the two slides (9). One section was stained as by a and b above and the adjacent section was stained by the aldehyde fuchsin technique (17) with woodstain scarlet and light green as a counterstain.…”

“…The cellular site of human growth hormone in human pituitary glands has previously been demonstrated (9). The present report concerns the localization of ACTH in human pituitaries.…”

“…Among the methods for demonstrating the cellular sites of production of anterior pituitary hormones are special histochemical techniques correlated with clinical and experimental findings (1)(2)(3), ultracentrifugation studies (4,5), tissue culture methods (6), and fluorescent antibody methods (7)(8)(9). The fluorescent antibody technique has the distinct advantages of specificity and of being applicable to pituitaries in histologic section.…”