Localization of eosinophil cationic protein, major basic protein, and eosinophil peroxidase in human eosinophils by immunoelectron microscopic technique.

Egesten, Arne; Alumets, J.; Mecklenburg, Claes von; Palmegren, M; Olsson, Inge

doi:10.1177/34.11.3772075

Cited by 81 publications

(44 citation statements)

References 16 publications

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“…1,50 Eosinophil peroxidase is a cationic protein normally localized within the matrix of eosinophil granules released in the extracellular space when eosinophils are activated, as shown in an in vitro anaphylactic model. 51,52 Similarly, an in vivo ultrastructural study of human gut eosinophils showed granular changes with the disappearance of the central core linked to cationic protein release. 18 Such evidence of eosinophil activation was described in outbreaks of other inflammatory digestive diseases, such as celiac disease and Crohn disease.…”

Section: Discussionmentioning

confidence: 99%

Activated eosinophils in upper gastrointestinal tract of patients with graft-versus-host disease

Daneshpouy

Socié

Lémann

et al. 2002

Blood

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Digestive tract damage during graft-versus-host reaction (GVHR) causes high morbidity and mortality. Diagnosis is often late because biopsies are performed when clinical signs are severe and pathologic markers of early inflammatory lesions are lacking. Eosinophils are inflammatory cells, cytotoxic in vitro to digestive epithelium; they are found in biopsy specimens taken during acute flare-ups of inflammatory bowel disease. We performed systematic duodenal biopsies immediately after digestive symptoms occurred and found a digestive GVHR incidence of 73.1% (n ‫؍‬ 93), higher than that found when digestive biopsies were performed immediately after severe clinical signs. Eosinophils were only present when there were histologic signs of GVHR; eosinophil presence correlated with GVHR severity. Electron microscopy with immunogold staining showed pathologic signs of in situ eosinophil activation, such as cytoplasmic granule alterations, and eosinophil peroxidase release in all patients. Interleukin-5 presence in activated eosinophils suggests eosinophil recruitment in digestive GVHR is an autocrine mechanism. Eosinophil density also correlated with GVHR severity, whether in acute or chronic clinical phases. Tissue eosinophils could thus be a marker of acute inflammatory flare-ups in GVHR. Systematic duodenal biopsy performed at the onset of digestive symptoms should allow early GVHR detection, and pathologic signs of GVHR, together with eosinophil density, might help modulate immunosuppressive therapy. (Blood. 2002;99:3033-3040)

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Section: Discussionmentioning

confidence: 99%

Activated eosinophils in upper gastrointestinal tract of patients with graft-versus-host disease

Daneshpouy

Socié

Lémann

et al. 2002

Blood

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“…Gr, Specific granules; N, nucleus; LB, lipid body. CCL5 (RANTES) [8]; CCL11 (eotaxin) [9]; ECP, eosinophil cationic protein [10]; EDN, eosinophilderived neurotoxin [11]; ENA-78, epithelial cell-derived neutrophil-activating peptide/ CXCL5 [12]; EPO, eosinophil peroxidase [10]; GM-CSF [13,14]; GRO-α, growth-related [44]. Numbers on the lower right corners of the panels indicate slice numbers A model for mobilization and transport of cytokines from secretory granules to the plasma membrane in human eosinophils.…”

Section: Concluding Remarks and Questions For The Futurementioning

confidence: 99%

Mechanisms of eosinophil secretion: large vesiculotubular carriers mediate transport and release of granule-derived cytokines and other proteins

Melo

Spencer

Dvorak

et al. 2007

Journal of Leukocyte Biology

106

113

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Eosinophils generate and store a battery of proteins, including classical cationic proteins, cytokines, chemokines, and growth factors. Rapid secretion of these active mediators by eosinophils is central to a range of inflammatory and immunoregulatory responses. Eosinophil products are packaged within a dominant population of cytoplasmic specific granules and generally secreted by piecemeal degranulation, a process mediated by transport vesicles. Large, pleiomorphic vesiculotubular carriers were identified recently as key players for moving eosinophil proteins from granules to the plasma membrane for extracellular release. During secretion, these specialized, morphologically distinct carriers, termed eosinophil sombrero vesicles, are actively formed and direct differential and rapid release of eosinophil proteins. This review highlights recent discoveries concerning the organization of the human eosinophil secretory pathway. These discoveries are defining a broader role for large vesiculotubular carriers in the intracellular trafficking and secretion of proteins, including selective receptor-mediated mobilization and transport of cytokines.

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“…Major basic protein (MBP) is a cationic protein stored in the core of the large crystalloid-containing granules of the eosinophil (5). In vitro incubation of human or guinea pig respiratory epithelium with purified MBP leads to a loss of its integrity (6)(7)(8), probably by a mechanism dependent on the high charge of this cationic protein (9).…”

Section: Introductionmentioning

confidence: 99%

In vivo neutralization of eosinophil-derived major basic protein inhibits antigen-induced bronchial hyperreactivity in sensitized guinea pigs.

Lefort

Nahori²,

Ruffié³

et al. 1996

J. Clin. Invest.

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Localization of eosinophil cationic protein, major basic protein, and eosinophil peroxidase in human eosinophils by immunoelectron microscopic technique.

Cited by 81 publications

References 16 publications

Activated eosinophils in upper gastrointestinal tract of patients with graft-versus-host disease

Activated eosinophils in upper gastrointestinal tract of patients with graft-versus-host disease

Mechanisms of eosinophil secretion: large vesiculotubular carriers mediate transport and release of granule-derived cytokines and other proteins

In vivo neutralization of eosinophil-derived major basic protein inhibits antigen-induced bronchial hyperreactivity in sensitized guinea pigs.

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