Localization of connexin43 in rat kidney

Barajas, Luciano; Liu, Li; Tucker, Mark A.

doi:10.1038/ki.1994.314

Cited by 64 publications

(68 citation statements)

References 12 publications

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“…Here, we document that, in their WT form, KI32 mice are normotensive and show, within the kidneys, the differential, cellspecific distribution of Cx43 and Cx32 that has been reported in the kidneys of other normal rodents (21,22,40). Thus, Cx32 was prominent among cells of proximal convoluted tubules, whereas Cx43 was mostly found in collecting medullary ducts (22); ECs of inter- and intralobular vessels, including the afferent arterioles (23,41); and glomerular capillaries.…”

Section: Figurementioning

confidence: 97%

“…This control is mostly achieved by the juxtaglomerular apparatus, which accommodates, in small regions of the kidney, several cell types, including smooth muscle, endothelial, mesangial, macula densa, and renin-producing cells of the afferent arterioles. Cells of each type are connected by gap junctions (16)(17)(18)(19)(20)(21)(22), and other gap junctions further link the ECs, the smooth muscle cells, and the renin-producing cells of the afferent arteriole (19,23,24). Connexin43 (Cx43) and Cx40 appear to be the prominent connexins forming these junctions in vivo (22,23).…”

Section: Introductionmentioning

confidence: 99%

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Connexin43-dependent mechanism modulates renin secretion and hypertension

Haefliger¹

2006

Journal of Clinical Investigation

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To investigate the function of Cx43 during hypertension, we studied the mouse line Cx43KI32 (KI32), in which the coding region of Cx32 replaces that of Cx43. Within the kidneys of homozygous KI32 mice, Cx32 was expressed in cortical and medullary tubules, as well as in some extra-and intraglomerular vessels, i.e., at sites where Cx32 and Cx43 are found in WT mice. Under such conditions, renin expression was much reduced compared with that observed in the kidneys of WT and heterozygous KI32 littermates. After exposure to a high-salt diet, all mice retained a normal blood pressure. However, whereas the levels of renin were significantly reduced in the kidneys of WT and heterozygous KI32 mice, reaching levels comparable to those observed in homozygous littermates, they were not further affected in the latter animals. Four weeks after the clipping of a renal artery (the 2-kidney, 1-clip [2K1C] model), 2K1C WT and heterozygous mice showed an increase in blood pressure and in the circulating levels of renin, whereas 2K1C homozygous littermates remained normotensive and showed unchanged plasma renin activity. Hypertensive, but not normotensive, mice also developed cardiac hypertrophy. The data indicate that replacement of Cx43 by Cx32 is associated with decreased expression and secretion of renin, thus preventing the renin-dependent hypertension that is normally induced in the 2K1C model.

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Section: Figurementioning

confidence: 97%

Section: Introductionmentioning

confidence: 99%

Connexin43-dependent mechanism modulates renin secretion and hypertension

Haefliger¹

2006

Journal of Clinical Investigation

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“…It seems most likely that the elevated Ang II may be a compensatory response to an initial blood pressure drop induced by NO. We note, however, that Cx43 might be more directly involved in the control of renin secretion from the juxtaglomerular apparatus (JGA) because Cx43 proteins are extremely abundant in kidney (26,27), where gap junctional communication is extensive, including connections between VSM and endothelium (20). Thus, the deletion of Cx43 in the endothelium might inhibit the communication between endothelium and JGA and result in an altered secretion of renin.…”

Section: Elevated Plasma No Concentration May Be a Primary Effect Ofmentioning

confidence: 99%

Endothelial cell-specific knockout of connexin 43 causes hypotension and bradycardia in mice

Liao

Day

Damon

et al. 2001

Proc. Natl. Acad. Sci. U.S.A.

209

207

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Connexin 43 (Cx43) is a protein expressed in a variety of mammalian tissues. However, the lack of specific blockers and the absence of known genetic mutants have hampered the investigation of the function of this protein. Cx43-null mice die shortly after birth, thus preventing functional studies in vivo. Here, we report the generation and characterization of a vascular endothelial cell-specific deletion of the Cx43 gene (VEC Cx43 KO) in mice by using the loxP͞Cre system. Using homologous recombination, a mouse line was created carrying loxP sites flanking exon 2 of the Cx43 gene (''floxed'' mice). To produce cell specific deletion of the Cx43 gene, these mice were crossed with animals from a line carrying the Tie 2-Cre transgene. The homozygous VEC Cx43 KO mice survived to maturity. However, they were hypotensive and bradycardic when compared with heterozygous VEC Cx43 KO mice, or to the floxed Cx43 gene mice. The hypotension was associated with marked elevation of plasma nitric oxide (NO) levels as well as elevated plasma angiotensin (Ang) I and II. We hypothesize that endothelial cell Cx43 plays a key role in the formation and͞or action of NO, and that the elevation of Ang II is a secondary event. The specific cellular basis for the hypotension remains to be established, but our findings support the idea that endothelial Cx43 gap junctions are involved in maintaining normal vascular function; moreover, these animals provide the opportunity to determine more clearly the role of endothelial Cx43 in vascular development and homeostasis.

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“…One of the striking features of MC is that these cells possess extremely high density of GJ (20,21). This unique property indicates a possibility that MC are actively involved in the function of the juxtaglomerular apparatus (22).…”

mentioning

confidence: 96%

“…This unique property indicates a possibility that MC are actively involved in the function of the juxtaglomerular apparatus (22). Like SMC, MC mainly express GJ protein connexin 43 (Cx43) (20). We previously reported that the GJ in MC are critically involved in the transmission of intercellular signal and in the coordination of MC contraction (23).…”

mentioning

confidence: 99%