Localization of Cathepsins B, D, L, LAMP-1 and .MU.-Calpain in Developing Hair Follicles.

Morioka, Kazuyuki; Sato-Kusubata, Kaori; Kawashima, Seiichi; Ueno, Takashi; Kominami, Eiki; Sakuraba, Hitoshi; Ihara, Setsunosuke

doi:10.1267/ahc.34.337

Cited by 10 publications

(7 citation statements)

References 27 publications

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“…Incubation of sections with primary antibodies prepared as described before was carried out for 24 hr at 4°C and then with MAX-PO for 30 min at room temperature. Sections colored by DAB were poststained briefly with methylgreen-pyronine [17]. In addition to the immunoperoxidase staining, some sections were stained with hematoxylin and eosin for histological examinations.…”

Section: Methodsmentioning

confidence: 99%

Localization of Myosin and Actin in the Pelage and Whisker Hair Follicles of Rat

Morioka

Matsuzaki

Takata

2006

Acta Histochem. Cytochem

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The combined effects of myosin II and actin enable muscle and nonmuscle cells to generate forces required for muscle contraction, cell division, cell migration, cellular morphological changes, the maintenance of cellular tension and polarity, and so on. However, except for the case of muscle contraction, the details are poorly understood. We focus on nonmuscle myosin and actin in the formation and maintenance of hair and skin, which include highly active processes in mammalian life with respect to the cellular proliferation, differentiation, and movement. The localization of nonmuscle myosin II and actin in neonatal rat dorsal skin, mystacial pad, hair follicles, and vibrissal follicles was studied by immunohistochemical technique to provide the basis for the elucidation of the roles of these proteins. Specificities of the antibodies were verified by using samples from the relevant tissues and subjecting them to immunoblotting test prior to morphological analyses. The myosin and actin were abundant and colocalized in the spinous and granular layers but scarce in the basal layer of the dorsal and mystacial epidermis. In hair and vibrissal follicles, nonmuscle myosin and actin were colocalized in the outer root sheath and some hair matrix cells adjoining dermal papillae. In contrast, most areas of the inner root sheath and hair matrix appeared to comprise very small amounts of myosin and actin. Hair shaft may comprise significant myosin during the course of its keratinization. These results suggest that the actin-myosin system plays a part in cell movement, differentiation, protection and other key functions of skin and hair cells.

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Section: Methodsmentioning

confidence: 99%

Localization of Myosin and Actin in the Pelage and Whisker Hair Follicles of Rat

Morioka

Matsuzaki

Takata

2006

Acta Histochem. Cytochem

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“…The strong expression of RNase A in the spinous layer and the steep decrease in 28S-rRNA from the spinous to the granular layer suggest the contribution of RNase A to the degradation of ribosomes. We have previ- ously reported that the immunohistochemical signals of several lysosomal and cytoplasmic proteases increase at the spinous layer [11]. These enzymes are expected to coordinate the conversion of cytoplasm from the proliferative to the terminal differentiation phase.…”

Section: Discussionmentioning

confidence: 96%

“…The epidermis, hair shaft, and inner root sheath serve as good models for the study of cellular differentiation processes, since their constituent cells are arranged in order according to differentiation stages from immature stem cells to terminally differentiated cells [4,9,11]. The epidermal and inner root sheath cells finally desquamate and the hair shaft epilates.…”

Section: Introductionmentioning

confidence: 99%

Degeneration of Ribosomes in Keratinizing Cells: Possible Participation of RNase A in the Skin and Hair of Rat

Morioka

2004

Acta Histochem. Cytochem

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“…It appears that both CtsB and CtsL are essential for HF maintenance. Both proteases are expressed in the external root sheath of rat HFs ( 37 ). CTSB and CTSL are also present in human bulge cells and outer root sheath cells, as identified by cDNA microarrays ( 38 ).…”

Section: Discussionmentioning

confidence: 99%

Cathepsin B as a potential cystatin M/E target in the mouse hair follicle

et al. 2017

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Deficiency of the cysteine protease inhibitor cystatin M/E (Cst6) in mice leads to disturbed epidermal cornification, impaired barrier function, and neonatal lethality. We report the rescue of the lethal skin phenotype of ichq (Cst6-deficient; Cst6−/−) mice by transgenic, epidermis-specific, reexpression of Cst6 under control of the human involucrin (INV) promoter. Rescued Tg(INV-Cst6)Cst6ichq/ichq mice survive the neonatal phase, but display severe eye pathology and alopecia after 4 mo. We observed keratitis and squamous metaplasia of the corneal epithelium, comparable to Cst6−/−Ctsl+/− mice, as we have reported in other studies. We found the INV promoter to be active in the hair follicle infundibulum; however, we did not observe Cst6 protein expression in the lower regions of the hair follicle in Tg(INV-Cst6)Cst6ichq/ichq mice. This result suggests that unrestricted activity of proteases is involved in disturbance of hair follicle biology, eventually leading to baldness. Using quenched activity-based probes, we identified mouse cathepsin B (CtsB), which is expressed in the lower regions of the hair follicle, as an additional target of mouse Cst6. These data suggest that Cst6 is necessary to control CtsB activity in hair follicle morphogenesis and highlight Cst6-controlled proteolytic pathways as targets for preventing hair loss.—Oortveld, M. A. W., van Vlijmen-Willems, I. M. J. J., Kersten, F. F. J., Cheng, T., Verdoes, M., van Erp, P. E. J., Verbeek, S., Reinheckel, T., Hendriks, W. J. A. J., Schalkwijk, J., Zeeuwen, P. L. J. M. Cathepsin B as a potential cystatin M/E target in the mouse hair follicle.

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Localization of Cathepsins B, D, L, LAMP-1 and .MU.-Calpain in Developing Hair Follicles.

Cited by 10 publications

References 27 publications

Localization of Myosin and Actin in the Pelage and Whisker Hair Follicles of Rat

Localization of Myosin and Actin in the Pelage and Whisker Hair Follicles of Rat

Degeneration of Ribosomes in Keratinizing Cells: Possible Participation of RNase A in the Skin and Hair of Rat

Cathepsin B as a potential cystatin M/E target in the mouse hair follicle

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