Localization of bFGF and FGF‐receptor in the developing nervous system of the embryonic and newborn rat

Weise, Brigitte; Janet, Thierry; Grothe, Claudia

doi:10.1002/jnr.490340409

Cited by 98 publications

(62 citation statements)

References 58 publications

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“…[1][2][3] Among these peptides, one of the best studied is the fibroblast growth factor-2 (FGF-2, basic FGF, bFGF), the prototype of a family of heparin binding growth factors. 4 FGF-2 is found at relatively high concentrations in the developing and in the adult brain, 5,6 and has strong trophic effects in central nervous system neurons and astrocytes. 7,8 FGF-2 has been shown to be implicated in the maturation, as well as in the survival and reorganization of neurons in the adult brain after injury.…”

Section: Introductionmentioning

confidence: 99%

Replication-defective herpes simplex virus vectors for neurotrophic factor gene transfer in vitro and in vivo

et al. 1999

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Section: Introductionmentioning

confidence: 99%

Replication-defective herpes simplex virus vectors for neurotrophic factor gene transfer in vitro and in vivo

et al. 1999

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“…In the central nervous system, FGF2 is expressed widely in neuronal and glial cells (4,5) and possesses neuroprotective effects both in vivo (6) and in vitro (7). FGF2 mediates its biological effects via binding and activation of specific high affinity tyrosine kinase receptors, named FGFR1-4 (8,9).…”

mentioning

confidence: 99%

Alternate FGF2-ERK1/2 Signaling Pathways in Retinal Photoreceptor and Glial Cells in Vitro

Kinkl

Sahel

Hicks

2001

Journal of Biological Chemistry

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Basic fibroblast growth factor (FGF2) stimulates photoreceptor survival in vivo and in vitro, but the molecular signaling mechanism(s) involved are unknown. Immunohistochemical and immunoblotting analyses of pure photoreceptors, inner retinal neurons, and Mü ller glial cells (MGC) in vitro revealed differential expression of the high affinity FGF receptors (FGFR1-4), as well as many cytoplasmic signaling intermediates known to mediate the extracellular signal-regulated kinase (ERK1/2) pathway. FGF2-induced tyrosine phosphorylation in vitro exhibited distinct profiles for each culture type, and FGF2-induced ERK1/2 activation was observed for all three preparations. Whereas U0126, a specific inhibitor of ERK kinase (MEK), completely abolished FGF2-induced ERK1/2 tyrosine phosphorylation and survival in cultured photoreceptors, persistent ERK1/2 phosphorylation was observed in cultured inner retinal cells and MGC. Furthermore U0126 treatment entirely blocked nerve growth factor-induced ERK1/2 activation in MGC, as well as FGF2-induced ERK1/2 activation in cerebral glial cells. Taken together, these data indicate that FGF2-induced ERK1/2 activation is entirely mediated by MEK within photoreceptors, which is responsible for FGF2-stimulated photoreceptor survival. In contrast, inner retina/glia possess alternative, cell type, and growth factor-specific MEK-independent ERK1/2 activation pathways. Hence signaling and biological effects elicited by FGF2 within retina are mediated by cell type-specific pathways.

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“…Moreover, FGF-2 immunoreactivity was demonstrated in cultured neuronal cells derived from DRG of chick and rat embryos, and also in developing rat DRG in vivo 30,31 .…”

Section: Discussionmentioning

confidence: 93%

S100ß and fibroblast growth factor-2 are present in cultured Schwann cells and may exert paracrine actions on the peripheral nerve injury

et al. 2008

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PURPOSE: The neurotrophic factor fibroblast growth factor-2 (FGF-2, bFGF) and Ca++ binding protein S100ß are expressed by the Schwann cells of the peripheral nerves and by the satellite cells of the dorsal root ganglia (DRG). Recent studies have pointed out the importance of the molecules in the paracrine mechanisms related to neuronal maintenance and plasticity of lesioned motor and sensory peripheral neurons. Moreover, cultured Schwann cells have been employed experimentally in the treatment of central nervous system lesions, in special the spinal cord injury, a procedure that triggers an enhanced sensorymotor function. Those cells have been proposed to repair long gap nerve injury. METHODS: Here we used double labeling immunohistochemistry and Western blot to better characterize in vitro and in vivo the presence of the proteins in the Schwann cells and in the satellite cells of the DRG as well as their regulation in those cells after a crush of the rat sciatic nerve. RESULTS: FGF-2 and S100ß are present in the Schwann cells of the sciatic nerve and in the satellite cells of the DRG. S100ß positive satellite cells showed increased size of the axotomized DRG and possessed elevated amount of FGF-2 immunoreactivity. Reactive satellite cells with increased FGF-2 labeling formed a ring-like structure surrounding DRG neuronal cell bodies.Reactive S100ß positive Schwann cells of proximal stump of axotomized sciatic nerve also expressed higher amounts of FGF-2. CONCLUSION: Reactive peripheral glial cells synthesizing FGF-2 and S100ß may be important in wound repair and restorative events in the lesioned peripheral nerves.

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Localization of bFGF and FGF‐receptor in the developing nervous system of the embryonic and newborn rat

Cited by 98 publications

References 58 publications

Replication-defective herpes simplex virus vectors for neurotrophic factor gene transfer in vitro and in vivo

Replication-defective herpes simplex virus vectors for neurotrophic factor gene transfer in vitro and in vivo

Alternate FGF2-ERK1/2 Signaling Pathways in Retinal Photoreceptor and Glial Cells in Vitro

S100ß and fibroblast growth factor-2 are present in cultured Schwann cells and may exert paracrine actions on the peripheral nerve injury

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