Localization of Arginine-Rich Proteins in Mature Seeds of some Members of the Gramineae

Fulcher, R. G.; O’Brien, T. P.; Simmonds, DH

doi:10.1071/bi9720487

Cited by 32 publications

(4 citation statements)

References 18 publications

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“…Both fresh, unfixed tissue and tissue that had been embedded for TEM were also used for light microscopy. As described by Maldonado and Lott (1991), the staining procedures used included : acid fuchsin (Sigma A3908) and Toluidine blue O (Sigma T 3260 CI 52040) (Feder and O'Brien, 1968) ; Coomassie brilliant blue (Sigma B 7920 CI 42660) (Gahan, 1984 ;Pearse, 1985) ; fast green FCF (Sigma F 7252 CI 42053) (Fulcher, O'Brien and Simmonds, 1972) ; iodine-potassium iodide (Sigma L 6146) and periodic acid-Schiff (PAS) preceded by aldehyde blockade with dimedone (Sigma D 3504) (O'Brien and McCully, 1981) ; and Sudan black B (Sigma S 2380 CI 26150) (Bronner, 1975). For 0305-7364\98\100481j08 $30.00\0…”

Section: Specimen Preparationmentioning

confidence: 99%

Seed Structure and Localization of Reserves inChenopodium quinoa

Prego¹,

Maldonado²,

1998

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The three areas of food reserves in quinoa seeds are : a large central perisperm, a peripheral embryo and a one to twocell layered endosperm surrounding the hypocotyl-radicle axis of the embryo. Cytochemical and ultrastructural analysis revealed that starch grains occupy the cells of the perisperm, while lipid bodies, protein bodies with globoid crystals of phytin, and proplastids with deposits of phytoferritin are the storage components of the cells of the endosperm and embryo tissues. EDX analysis of the endosperm and embryo protein bodies revealed that globoid crystals contain phosphorus, potassium and magnesium. These results are compared with studies on other perispermous seeds published to date.

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Section: Specimen Preparationmentioning

confidence: 99%

Seed Structure and Localization of Reserves inChenopodium quinoa

Prego¹,

Maldonado²,

1998

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“…Flowers fìxed in F AA (formalin-acetic acid-ethylic alcohol) were embedded in paraffin and sectioned. Sections were cut at 8-10 Jlm and stained with iodine-potassium i odi de and periodic acid-Schiff (PAS) (O'Brien & McCully 1981 ); fast green FCF (Fulcher et al 1972); Sudan black (Bronner 1975); ruthenium red, Coomassie brilliant blue (Pearse 1985); acid fushsin and toluidine blue O (Feder & O' Brien 1968).…”

Section: Materials Ano Methodsmentioning

confidence: 99%

Secretory tissues of the flower ofSanango racemosum(Gesneriaceae). I. Light microscopy

Maldonado¹,

Otegui

1997

Acta Botanica Neerlandica

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SUMMARY Sanango racemosum (Ruiz & Pay.) Barringer has a dry stigma without a free‐flowing secretion fluid but with a hydrated proteinaceous pellicle. The stigmatic surface is covered with unicellular, bottle‐shaped papillae. At maturity, a viscous emulsion is accumulated between the cuticle and the pecto‐cellulosic wall of the papillae, causing it to become detached from the surface of the papilla cell walls. The style has a central solid core of transmitting tissue. The cells of the transmitting tissue are rich in starch and exhibit thick lateral walls rich in pectic substance. The nectary disk is a ring elongated into a cup, with five lobes at the top. One of the most conspicuous histological features of the disk is the abundance of starch in the secretory cells. The disk is supplied only by phloem; the stomata are found in the top of the lobes. A fluid substance is produced just before anthesis and secreted through the stomata with no visible decline in starch level. During anthesis and after fertilization, a rapid decline in starch is observed. The hypothesis that the disk has other functions besides that of a nectary is discussed.

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“…Some flowers were embedded in Spurr's resin and sectioned at 1-2 pn. The staining procedures also included: acid fuchsin and toluidine blue (Pearse 1985); fast green FCF (Fulcher et al 1972); iodine potassium iodide and periodic acid-Schiff (PAS) (O'Brien & McCully 1981).…”

Section: Specimen Preparation Ovulementioning

confidence: 99%

Embryological studies in Sanango racemosum (Loganiaceae s.l.)

Maldonado¹,

Otegui

Bozini³

1998

Nordic Journal of Botany

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An embryological study was undertaken in the Amazonian genus Sanango in an attempt to help clarify the relationship of the genus. Detailed description of microsporangium, microsporogenesis, microspore, ovule, megasporogenesis, embryo sac and development of the endosperm are presented. The results confirm that Sanango is undoubtedly related to the Scrophulariales but it remains to be determined what the most satisfactory assignment of the genus should be. On the basis of early endosperm development, Sanango appears as closely related to the Gesneriaceae as well to the Scrophulariaceae. Further studies of embryonic stages and seed coat development would be helpful in clarifying this relationship. The other embryological features studied here seem not to be of taxonomic importance. A closer relationship with the Loganiaceae (Gentianales) is improbable.

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Localization of Arginine-Rich Proteins in Mature Seeds of some Members of the Gramineae

Cited by 32 publications

References 18 publications

Seed Structure and Localization of Reserves inChenopodium quinoa

Seed Structure and Localization of Reserves inChenopodium quinoa

Secretory tissues of the flower ofSanango racemosum(Gesneriaceae). I. Light microscopy

Embryological studies in Sanango racemosum (Loganiaceae s.l.)

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