Localization of androgen receptor and cytochrome P450 aromatase in the follicle and corpus luteum of the porcine ovary

Słomczyńska, Maria; Tabarowski, Zbigniew

doi:10.1016/s0378-4320(00)00225-6

Cited by 71 publications

(47 citation statements)

References 36 publications

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“…We were able to show that the intensity of immunostaining reached its maximum in the FSH-stimulated granulosa cells and was comparatively weak in nonstimulated cultures. These findings are in agreement with our previous study [22]. We demonstrated that the expression of P450 aromatase mRNA is dependent on follicular development stage, being at a very low level in the small follicles and increased in the medium and large ones, respectively.…”

Section: Discussionsupporting

confidence: 94%

“…We demonstrated that the expression of P450 aromatase mRNA is dependent on follicular development stage, being at a very low level in the small follicles and increased in the medium and large ones, respectively. Also further findings [23] clearly showed a weak aromatase immunostaining in the small porcine follicles and strong intensity of immunoreaction in the granulosa cells of the preovulatory follicles, either mural or antral layers. Our present study provides immunocytochemical and radioimmunological evidence that incubation of granulosa cells with FSH and testostosterone induced aromatase activity that resulted in the most intensive staining for aromatase and consecutively higher secretion of estradiol.…”

Section: Discussionmentioning

confidence: 68%

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Effects of transferrin on aromatase activity in porcine granulosa cells in vitro.

Durlej

Duda²,

Knapczyk³

et al. 2009

Folia Histochem Cytobiol.

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Abstract:Proliferating cells have an absolute requirement for iron, which is delivered by transferrin with subsequent intracellular transport via the transferrin receptor. Recent studies have reported that transferrin plays a crucial role in the local regulation of ovarian function, apart from its iron-binding characteristic. Therefore, the present study was undertaken to explore the possible role of transferrin in porcine granulosa cells function by examining its influence on aromatase activity, the most important indicator of follicular cell differentiation. In the first series of studies, pig granulosa cells isolated from small, immature follicles were cultured in the presence of transferrin alone (10 µg/ml or 100 µg/ml) or with the addition of FSH (100ng/ml). The second series of studies was undertaken to determine transferrin-stimulated granulosa cells ability to aromatize exogenous testosterone (1×10 -7 M). One hour after the establishment of cultures an aromatase inhibitor CGS16949A was added to test its influence on estradiol production. After 48 hours, cultures were terminated and cells were processed for immunocytochemical staining of aromatase. Media were frozen for further estradiol level analysis. Positive immunostaining for aromatase was found in all granulosa cell cultures. The intensity of immunostaining was always stronger in cultures supplemented with FSH whereas the addition of transferrin had no effect. Granulosa cells in vitro synthesized the highest amount of estradiol after the addition of FSH and exogenous testosterone as measured radioimmunologically. Concomitant treatment with FSH and transferrin caused an inhibition of FSH-stimulated aromatase activity. The production of estradiol also declined in the presence of FSH, testosterone and transferrin. This study demonstrates that transferrin had a dose-dependent inhibitory effect on FSH-stimulated aromatase activity, which was confirmed by radioimmunoassay. Our results indicate that transferrin may be an important factor in the regulation of granulosa cell diferentiation.

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Section: Discussionsupporting

confidence: 94%

Section: Discussionmentioning

confidence: 68%

Effects of transferrin on aromatase activity in porcine granulosa cells in vitro.

Durlej

Duda²,

Knapczyk³

et al. 2009

Folia Histochem Cytobiol.

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“…Light-microscopic observation showed that the cellular localization of P450 scc , 3β-HSD and P450 arom in steroidogenic cells of follicles and CL in the control group is in agreement with results of previous studies performed on ovaries of gilts and goats, with exception of the presence of P450 arom in cells of CL [28][29][30][31][32][33]. In turn, the distribution of studied enzymes in the structures of cystic ovaries has changed.…”

Section: Discussionsupporting

confidence: 89%

Expression of steroidogenic enzymes in porcine polycystic ovaries.

Kozłowska

Majewski²,

Jana³

2009

Folia Histochem Cytobiol.

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Abstract:In the present study the expression pattern of the cholesterol side-chain cleavage cytochrome (P450 scc ), 3β-hydroxysteroid dehydrogenase (3β-HSD) and aromatase (P450 arom ) was analyzed in the health and polycystic ovaries of gilts by means of the Western blot and immunohistochemistry. The polycystic status of ovaries was induced by i.m. dexamethasone (DXM) injections on days 7-21 of the estrous cycle. Macroscopic observation of ovaries of DXM-treated gilts revealed the presence of cysts (1-2 cm in diameter, with a mean number of 7.0±1.2 per ovary), a decrease (P<0.05) in number of small follicles (1-3 mm in diameter), as well as the lack of medium-sized follicles (4-6 mm in diameter) and corpora lutea, as compared to the control animals. The expression of P450 scc (P<0.01), 3β-HSD (P<0.05) and P450 arom (P<0.001) proteins in the cysts was higher than in the medium-sized follicles of the control gilts. Moreover, DXM injections resulted also in an enhancement (P<0.05) in the level of P450 scc protein in the walls of small follicles as compared to the control gilts. Following DXM administration the immunoreactivity (IR) of P450 scc in the primordial follicles was lower than in the control group. Comparing to the control gilts, the reaction for this enzyme in DXM-treated animals was observed in secondary follicles, while for 3β-HSD, in primordial, primary, as well as secondary follicles. The immunostaining for P450 scc (theca cells) and P450 arom (granulosa cells) in the small follicles of the DXM-treated gilts were more prominent than those found in the gonads of control animals. However, IR for P450 scc was not found in the granulosa cells of small follicles in the gilts receiving DXM. The intensity of P450 scc and P450 arom labelling was distinctly enhanced in the cysts as compared to the medium follicles of the control animals. Furthermore, in contrary to the medium follicles of the control animals, faint IR for 3β -HSD was found in the granulosa cell layer of cysts. Our data revealed that both the expression of P450 scc , 3β-HSD and P450 arom and localization of these enzymes in polycystic ovaries were different from those, found under physiological conditions. These results suggested that above-mentioned enzymes may, by influencing the ovarian steroid synthesis, play an essential role in the creation and/or course of cystic ovarian disease.

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“…Studies across species reported that ARs are expressed in theca cells, granulosa cells (GCs), and the oocyte of the follicle and throughout most stages of follicular development (Horie et al 1992, Chadha et al 1994, Hirai et al 1994, Suzuki et al 1994, Tetsuka & Hillier 1996, Szoltys & Slomczynska 2000, Slomczynska & Tabarowski 2001, Gill et al 2004, Hampton et al 2004, Juengel et al 2006. However, their expression patterns may differ between ovarian cell types (Tetsuka & Hillier 1996, Szoltys & Slomczynska 2000 and in most species, AR is abundant in the preantral/antral stages of follicular development but declines as follicles mature to the preovulatory stage (Horie et al 1992, Chadha et al 1994, Suzuki et al 1994, Duffy et al 1999, Hampton et al 2004, Juengel et al 2006.…”

Section: Ar Expression and Regulationmentioning

confidence: 99%

Androgen actions in the ovary: balance is key

Prizant

Gleicher

Sen

2014

Journal of Endocrinology

116

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For many decades, elevated androgens in women have been associated with poor reproductive health. However, recent studies have shown that androgens play a crucial role in women's fertility. The following review provides an overall perspective about how androgens and androgen receptor-mediated actions regulate normal follicular development, as well as discuss emerging concepts, latest perceptions, and controversies regarding androgen actions and signaling in the ovary.

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Localization of androgen receptor and cytochrome P450 aromatase in the follicle and corpus luteum of the porcine ovary

Cited by 71 publications

References 36 publications

Effects of transferrin on aromatase activity in porcine granulosa cells in vitro.

Effects of transferrin on aromatase activity in porcine granulosa cells in vitro.

Expression of steroidogenic enzymes in porcine polycystic ovaries.

Androgen actions in the ovary: balance is key

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