Localization of an Aldo-Keto Reductase (AKR2E4) in the Silkworm Bombyx mori (Lepidoptera: Bombycidae)

Yamamoto, Kohji; Ozakiya, Yusuke; Uno, Tomohide

doi:10.1093/jisesa/iex071

Cited by 7 publications

(5 citation statements)

References 14 publications

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“…In vitro experiments, performed from the testes of several moth species including A. ipsilon, have highlighted that this organ is able spontaneously to produce a variety of ecdysteroids and in particular 20E which is known to initiate the spermatogenesis [41,51]. In line with this finding, a recent immunological study has unveiled the presence of ecdysteroidogenic enzymes within the testicular sperm cells and spermatocytes in the moth B. mori [52]. Considering all the above data, AiHR38 could play a role in regulating testicular activity in A. ipsilon males, probably by interfering on the ecdysteroidogenesis, similar to the action of its NR4A1 homologue.…”

Section: Discussionmentioning

confidence: 81%

The insect HR38 nuclear receptor, a member of the NR4A subfamily, is a synchronizer of reproductive activity in a moth

et al. 2018

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In the male moth, Agrotis ipsilon, the behavioural response and neuron sensitivity within the olfactory centres, the antennal lobes (ALs), to female sex pheromone increase with age, in correlation with the maturation of sex accessory glands (SAGs). By contrast, newly mated males cease to be attracted to sex pheromone and remate when their SAGs are refilled during the next night. The insect hormone receptor 38 (HR38), an ortholog of the vertebrate NR4A receptors, is a component of ecdysteroid signalling pathway which controls adult male physiology and behaviour. Here, we cloned the A. ipsilon HR38 (AiHR38) and explored its function in the coordination of reproductive events in the male. AiHR38 was detected in SAGs and ALs, and where its amount raised with age, in parallel with SAG protein content and sex pheromone responsiveness. By contrast, the AL and SAG AiHR38 expressions declined at 0-2 h after mating, in linking with depletion of SAG protein reserves and loss of sensitivity to sex pheromone. The increased AL and SAG AiHR38 expressions at 20-24 h postmating coincided with replenishing of SAGs and recovery of sensitivity to sex pheromone for a new mating. Moreover, AiHR38 knockdown resulted in reduction in SAG protein amount and disruption of sex pheromone-orientated flight. These results show that the insect HR38 is essential both for SAG activity, probably by controlling the protein synthesis, and display of male sexual behaviour, and that the concomitant regulation of its expression within SAGs and olfactory centres contributes to synchronisation between fertility and sexual activity. DATABASE: The nucleotide sequence of Agrotis ipsilon HR38 is available in the DDBJ/EMBL/GenBank databases under the accession number MF402845.

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Section: Discussionmentioning

confidence: 81%

The insect HR38 nuclear receptor, a member of the NR4A subfamily, is a synchronizer of reproductive activity in a moth

et al. 2018

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“…We also observed that myrosinase 1 and arylsulfatase B were upregulated after both sugar‐mimic alkaloid treatments (Figure 4b). Moreover, Aldo‐Keto Reductase (AKR2E4), plays an important role in 3‐dehydroecdysone conversion to ecdysone and spermatogenesis in silkworm testes (Yamamoto, Ozakiya, & Uno, 2017). AKR2E4 was also upregulated after both types of sugar‐mimic alkaloid treatments.…”

Section: Resultsmentioning

confidence: 99%

Physiological adaptations to sugar‐mimic alkaloids: Insights from Bombyx mori for long‐term adaption and short‐term response

Jia

Dai

et al. 2020

Ecology and Evolution

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Insects evolved adaptive plasticity to minimize the effects of the chemical defenses of their host plants. Nevertheless, the expressional response and adaptation of phytophagous specialists for long‐term adaption and short‐term response to host phytochemicals remains largely unexplored. The mulberry (Morus alba)–silkworm (Bombyx mori) interaction is an old and well‐known model of plant–insect interaction. In this study, we examined the long‐term adaption and short‐term response of the mulberry‐specialist silkworm to two sugar‐mimic alkaloids in mulberry: the commonly encountered 1‐deoxynojirimycin (1‐DNJ) and occasionally encountered 1,4‐dideoxy‐1,4‐imino‐D‐arabinitol (D‐AB1), respectively. Global transcriptional patterns revealed that the physiological responses induced by the selective expression of genes involved in manifold cellular processes, including detoxification networks, canonical digestion processes, target enzymes, and other fundamental physiological processes, were crucial for regulating metabolic homeostasis. Comparative network analysis of the effects of exposure to D‐AB1 and 1‐DNJ supported the contention that B. mori produced similar and specific trajectories of changed gene expression in response to different sugar‐mimic alkaloids. D‐AB1 elicited a substantial proportion of downregulated genes relating to carbohydrate metabolism, catabolic process, lipid metabolism, and glycan biosynthesis and metabolism. This study dramatically expands our knowledge of the physiological adaptations to dietary sugar‐mimic alkaloid intake and uncovered both metabolic evolutionarily responses and unique adaptive mechanisms previously unknown in insects.

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“…Increased ability to metabolize specific steroids during testicular development, such as increased expression of Aldo-keto reductase family 1 member C3, the same family member as Aldo-keto reductase family 1 member A1, during testicular development in domestic cats 32 . The level of Aldo-Keto Reductase mRNA in silkworm testis is higher than that in other tissues, which plays an important role in the spermatogenesis of silkworm 33 . Pachytene checkpoint protein 2 homolog was searched by Uniprot database.…”

Section: Differential Proteinmentioning

confidence: 89%

Comparison of urinary proteome in the first two days after mating in male rats

Wang,

Zhao,

Gao

2023

Preprint

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ObjectiveTo compare urine proteome changes in the first two days after mating behavior in male rats.MethodsThe urine samples of Sprague-Dawley rats on the day of mating and the day after mating were collected and analyzed by non-label quantitative proteomics by high performance liquid chromatography-tandem mass spectrometry (LC-MS/MS), and the differential proteins in the urine proteome were screened protein function and biological pathways were analyzed.Results43 differential proteins were identified by comparing the urine proteome of rats on the next day of mating with that on the day of mating, and nearly two-thirds of the differential proteins were related to spermatogenesis.ConclusionsThere were changes in the urinary proteome on the next day of mating compared with the day of mating, and some of the proteins whose functions were known to be related to spermatogenesis. Urine proteome has the potential to reflect spermatogenesis.

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Localization of an Aldo-Keto Reductase (AKR2E4) in the Silkworm Bombyx mori (Lepidoptera: Bombycidae)

Cited by 7 publications

References 14 publications

The insect HR38 nuclear receptor, a member of the NR4A subfamily, is a synchronizer of reproductive activity in a moth

The insect HR38 nuclear receptor, a member of the NR4A subfamily, is a synchronizer of reproductive activity in a moth

Physiological adaptations to sugar‐mimic alkaloids: Insights from Bombyx mori for long‐term adaption and short‐term response

Comparison of urinary proteome in the first two days after mating in male rats

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