Localization of Alkaline Phosphatase and Ca2+-ATPase in the Cat Placenta

Champion, E. E.; Glazier, Jocelyn D.; Greenwood, Susan; Mann, Sarah; Rawlings, John M.; Sibley, Colin P.; Jones, Carolyn J.P.

doi:10.1053/plac.2002.0952

Cited by 16 publications

(4 citation statements)

References 24 publications

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“…Both decidual cells and maternal capillary endothelial cells were positive for TAUT. This finding parallels the localization of alkaline phosphatase to decidual and maternal endothelial cells (8) and further suggests a role for these cells in placental transport in this species.…”

Section: Discussionsupporting

confidence: 80%

System β and System A amino acid transporters in the feline endotheliochorial placenta

Champion

Mann²,

Glazier³

et al. 2004

American Journal of Physiology-Regulatory, Integrative and Comp

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Section: Discussionsupporting

confidence: 80%

System β and System A amino acid transporters in the feline endotheliochorial placenta

Champion

Mann²,

Glazier³

et al. 2004

American Journal of Physiology-Regulatory, Integrative and Comp

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“…The increase in binding of PAA after neuraminidase pretreatment indicated that many of these residues were subterminal to sialic acids. The basal plasma membrane of the human and cat placenta is also known to be active in substance transport and is rich in enzymes such as the calcium pump [Borke et al, 1989;Champion et al, 2003]. A particularly striking feature of this study was the unexpected and selective staining of the basal surface of the syncytiotrophoblast in hyena and human, demonstrating very dramatically the chemical diversity of the two faces of this tissue.…”

Section: Discussionmentioning

confidence: 66%

Glycosylation at the Fetomaternal Interface in Hemomonochorial Placentae from Five Widely Separated Species of Mammal: Is There Evidence for Convergent Evolution?

Jones

Carter

Aplin

et al. 2007

Cells Tissues Organs

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Hemomonochorial placentation occurs in diverse species. We have examined placental glycosylation in five widely separated mammals with this type of placentation – lesser hedgehog tenrec (Echinops telfairi), spotted hyena (Crocuta crocuta), nine-banded armadillo (Dasypus novemcinctus), human (Homo sapiens) and guinea pig (Cavia porcellus) – in order to assess whether evolutionary convergence to the hemomonochorial state is accompanied by a similar convergence of glycan expression. Placentae from 2 E. telfairi, 3 C. crocuta, 1 D. novemcinctus, 4 womenand 1 C. porcellus were fixed and processed into epoxy resin. Binding of twenty-three lectins was assessed using a semiquantitative ranking system. The trophoblast apical/microvillous membrane of all five species showed marked similarities in glycosylation. In the N-linked series, there were abundant bi/tri-antennary complex chains, while the non-bisected variants were much scarcer. All species had plentiful N-acetyl lactosamine sequences; at chain termini, binding to Galβ1,4GlcNAc and Galβ1,3GalNAc sequences was greatly enhanced after neuraminidase treatment. In all species, terminal NeuNAcα2,3 residues were detected. The tenrec had unusually abundant terminal N-acetyl galactosamine. The basal plasma membrane/basal lamina showed glycosylation patterns distinct from the microvillous membrane in each case, indicating chemical diversity of the two opposite faces of trophoblast. Similar classes of glycan at the hemochorial interface suggest conservation of function. The observed lectin binding patterns suggest broad similarities of glycosylation that may have arisen by convergent evolution.

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“…Blocking sera was removed from the slides, and unrinsed sections were quenched with peroxidase blocking reagent. Alternatively, dehydrated sections untreated with sera were quenched for 30 min with a solution of 2 ml 30% H 2 O 2 a and 400 ml of methanol 2 . Quenched sections were blocked with 5% (wt/vol) nonfat dry milk in TB for 1 hr and 15 min.…”

Section: Methodsmentioning

confidence: 99%

An Immunohistochemical Assay to Detect Trophoblasts in Frozen Feline Placenta

Scott

Wallace

Mays³

et al. 2011

J VET Diagn Invest

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Abstract. The placenta, a fetal endocrine organ, is composed of subpopulations of trophoblasts, including cytotrophoblasts, and syncytiotrophoblasts. Trophoblastic populations can be distinguished based upon their expression of cytokeratin. The purpose of the current study was to develop an immunohistochemistry (IHC) method to identify trophoblasts selectively in frozen feline placental tissue using antibodies specific for cytokeratin. The mouse monoclonal antibody anti-human pan-cytokeratin AE1/AE3 and a commercial detection system were used. Nonspecific immunoreactivity was encountered that could not be eliminated with altered blocking methods. The nonspecific reactivity was attributed to the goat anti-mouse/rabbit immunoglobulin G (IgG) peroxidase polymer included in the commercial kit. Alternatively, a polyclonal rabbit anti-cow cytokeratin wide spectrum screening antibody with goat anti-rabbit IgG polyclonal secondary antibody was used to detect cytokeratin in feline placental tissue. The IHC procedure eliminated nonspecific immunoreactivity while specifically labeling cytokeratin. This new approach provides an IHC method to identify trophoblasts specifically in feline placenta.

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Localization of Alkaline Phosphatase and Ca2+-ATPase in the Cat Placenta

Cited by 16 publications

References 24 publications

System β and System A amino acid transporters in the feline endotheliochorial placenta

System β and System A amino acid transporters in the feline endotheliochorial placenta

Glycosylation at the Fetomaternal Interface in Hemomonochorial Placentae from Five Widely Separated Species of Mammal: Is There Evidence for Convergent Evolution?

An Immunohistochemical Assay to Detect Trophoblasts in Frozen Feline Placenta

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