Localization of adrenomedullin-like immunoreactivity in the hypothalamo-hypophysial system of amphibians

González, Agustı́n; Marı́n, Oscar; Sánchez-Camacho, Cristina; Peña, Juan José; Zudaire, Enrique; Martı́nez, Alfredo; Cuttitta, Frank; Muñoz, Margarita

doi:10.1016/s0304-3940(98)00033-0

Cited by 17 publications

(8 citation statements)

References 18 publications

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“…These results suggest that the axons of the AMi neurons in the hypothalamus may terminate in the neurohypophysis. Noteworthy, in anuran and urodele amphibians the largest population of AMi cell bodies is also found in the hypothalamus (Gonzá lez et al, 1998;present study), mainly in the suprachiasmatic and infundibular regions. AMi cell bodies in the magnocellular nucleus were also observed in the frog, although they are not as abundant as in the salamander (Gonzá lez et al, 1998).…”

Section: Comparison With Previous Data Of Am-immunoreactivity In Mammalssupporting

confidence: 50%

“…However, the comparative analysis of the distributions of these neuropeptides with that of AM showed marked differences, pointing to a lack of crossreactivity of the antiserum used with those peptides. In the case of the hypothalamus, preliminary observations on anuran and urodeles showed a possible overlap between AM and AVT or NPY in the magnocellular and suprachiasmatic regions (Gonzá lez et al, 1998). The putative colocalization was studied in the present account and, in addition, the relationship between AMi cells and the catecholaminergic neurons (THi cells).…”

Section: Discussionmentioning

confidence: 80%

“…However, AM appears to have an early ancestral history based on immunological conserved epitopes of AM-like peptides found in echinoderms , fish, amphibians and reptiles (López and Cuesta, 1998). In a preliminary report, AM-like immunoreactivity in the hypothalamus of amphibians was demonstrated (Gonzá lez et al, 1998) and, recently, we have reported a wide distribution of AM-like immunoreactivity in the frog brain (Muñ oz et al, 2001).…”

Section: Introductionmentioning

confidence: 81%

“…The specificity of the antiserum was confirmed by pre-absorption of the serum with the synthetic peptide (1 nmol per ml of diluted antiserum). Examination for cross-reactivity was accomplished using other peptides including gastrin, cholecystokinin, gastrin-releasing peptide, arginine vasopressin, vasoactive intestinal peptide, oxytocin, calcitonin, CGRP, and neuropeptide Y (NPY) (see Serrano et al, 2000) The antiserum with the highest titer for AM and minimal cross-reactivity with other peptides was selected for immunohistochemical studies in diverse tissues including the nervous system (Gonzá lez et al, 1998;Martínez et al, 1996;Montuenga et al, 1997;Serrano et al, 2000). Moreover, the specificity of the antiserum has been tested in pancreatic tissue of diverse vertebrate species, including amphibians (López and Cuesta., 1999).…”

Section: Immunohistochemical Proceduresmentioning

confidence: 99%

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Comparative analysis of adrenomedullin‐like immunoreactivity in the hypothalamus of amphibians

Muñoz

López

Sánchez-Camacho

et al. 2001

Microscopy Res & Technique

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Adrenomedullin (AM) is a novel neuropeptide with special significance in the mammalian hypothalamo-hypophysial axis. By using an antiserum specific for human AM, we have studied the localization of AM-like immunoreactive (AMi) cell bodies and fibers in the hypothalamus and hypophysis of the amphibians Rana perezi (anuran), Pleurodeles waltl (urodele), and Dermophis mexicanus (gymnophionan). Distinct AMi cell groups were found for each species. In the anuran, six cell groups were localized in the preoptic and infundibular regions, whereas only three and one were found in the urodele and gymnophionan, respectively. A comparative analysis of AMi cells and cells expressing arginine vasotocin (AVT), neuropeptide Y (NPY), and tyrosine hydroxylase (TH) revealed strong differences between species. Thus, colocalization of AVT/AM is most likely to occur in the preoptic magnocellular nucleus of urodeles and it is reflected by the intense AM immunoreactivity in the neural lobe of the hypophysis. Colocalization of NPY/AM seems to be possible in the suprachiasmatic nucleus of anurans. In the gymnophionan, cells containing AVT and NPY are distinct from AMi cells. Only in anurans, the ventral aspect of the suprachiasmatic nucleus possesses a small population of AMi cells that express also TH immunoreactivity and most likely also express NPY. The results strongly suggest that AM in amphibians plays an important regulatory role in the hypothalamo-hypophysial system, as has been demonstrated in mammals. On the other hand, substantial differences have been found between species with respect to the degree of colocalization with other chemical substances.

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Section: Comparison With Previous Data Of Am-immunoreactivity In Mammalssupporting

confidence: 50%

Section: Discussionmentioning

confidence: 80%

Section: Introductionmentioning

confidence: 81%

Section: Immunohistochemical Proceduresmentioning

confidence: 99%

See 2 more Smart Citations

Comparative analysis of adrenomedullin‐like immunoreactivity in the hypothalamus of amphibians

Muñoz

López

Sánchez-Camacho

et al. 2001

Microscopy Res & Technique

Self Cite

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“…AM is found in picomolar concentrations in plasma which rise in conditions such as renal failure, hypertension, and septic shock (19,35). AM is produced by a wide variety of tissues and cells, and it has been demonstrated to accumulate in the apical regions of the normal human bronchial epithelium, in human skin, and in the skin of the Xenopus laevis toad (15,19,23,31,32). Cameron and Fleming demonstrated the localization of AM mRNA in epithelial cells lining the uterus, bronchioles, and gastrointestinal tract in mice and rats and also indicated novel roles for AM, possibly as an antimicrobial agent (6).…”

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confidence: 99%

Adrenomedullin Expression by Gastric Epithelial Cells in Response to Infection

Allaker

Kapas

2003

Clin Vaccine Immunol

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Adrenomedullin as a pancreatic hormone

López

Cuesta

2002

Microscopy Res & Technique

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Adrenomedullin (AM) is a multiregulatory peptide which is expressed in a wide range of tissues. In the pancreas, AM was first found in mammals, including man, and its colocalization with the pancreatic polypeptide (PP) was established in islet F cells. In addition, three different AM receptors have been characterized in B-cells. AM has been also located in the pancreatic cells of other vertebrate classes. The frequency and distribution of AM cells vary between different animals; they can be found scattered among the exocrine tissue, in the islets, or in ductal epithelia. The colocalization of AM with other hormones presents different patterns, although in birds, as in mammals, it seems to colocalize only with PP. The best-determined pancreatic AM function is the inhibition of insulin secretion in B-cells, which seems to be linked to a recently discovered binding protein, factor H. In relation to this physiological role, clinical data show that AM is raised in some groups of both types I and II diabetic patients and AM might have triggered the disease in a subset of them. On the other hand, AM pancreatic cells are also involved in the response to septic shock by increasing AM circulating levels. A third putative function is the inhibition of amylase secretion by the exocrine pancreatic cells. AM has been found in embryonic mammalian pancreas from the earliest stages of the development, colocalizing with all pancreatic hormones, although in adults only coexpression with PP is kept. AM may play a role in the growth and morphogenesis of the pancreas.

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Localization of adrenomedullin-like immunoreactivity in the hypothalamo-hypophysial system of amphibians

Cited by 17 publications

References 18 publications

Comparative analysis of adrenomedullin‐like immunoreactivity in the hypothalamus of amphibians

Comparative analysis of adrenomedullin‐like immunoreactivity in the hypothalamus of amphibians

Adrenomedullin Expression by Gastric Epithelial Cells in Response to Infection

Adrenomedullin as a pancreatic hormone

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