Localization of acid phosphatase in Saccharomyces cerevisiae: a clue to cell wall formation

Abstract

Acid phosphatase is present in two layers of the cell envelope of Saccharomyces cerevisiae. These are separated by another layer, which is free of acid phosphatase. We have evidence that the cell wall is built up in two stages, which are independent. In the first stage, the cell wall is built up during the formation of the bud. Glucanase vesicles are involved in this process. In the second stage, a thick layer is deposited at the inside against the new cell wall. This results in the thick, rigid wall of the mature yeast cell. This latter layer is probably assembled on the outer surface of the plasmalemma.

Purification and properties of a glycoprotein acid phosphatase from the yeast form of Yarrowia lipolytica

“…lipolytica occurs in a similar way to that described for S. cerevisiae (FIELD aqd SCHEKMAN 1980). The enzyme (as in S. cerevisiae, LINNEMANS et al 1977) is mainly located in the cell wall as can be deduced from the results shown in Fig. 1.…”

In Saccharomyces cerevisiae a short amino acid sequence facilitates excretion in the growth medium of periplasmic proteins

“…Partitioning within the cell-wall layers has been directly demonstrated for acid phosphatase, an enzyme that can be released into the growth medium (Haguenauer-Tsapis, 1992). Immuno-electron microscopy has shown that the enzyme is localized in two cell-wall layers: one is immediately next to the plasma membrane and the other is on the cell-wall surface (Linnemans et al, 1977). Enzymes at the outer surface of the wall probably can be released in the medium more easily than those in the inner layer.…”

A new method for the cytochemical demonstration of phosphatase activities in yeasts based on the use of cerous ions

“…Acid phosphatase is well-known as a constituent of the vacuole and, depending on conditions of growth, also in the cell wall of yeasts [16,19,20]. Working with phosphatase-derepressed cells of S. cerevisiae, Linnemans et al [21] showed that acid phosphatase was localized in two layers of the cell wall. In addition, in acid phosphatase secreting protoplasts of this organism the enzyme was also present in the entire endomembrane system [22].…”