Localization of a specific germ cell marker, DAZL1, in testicular germ cell neoplasias

Lifschitz-Mercer, Beatriz; Elliott, David J.; Issakov, Josephine; Leider-Trejo, Leonor; Schreiber, Letizia; Misonzhnik, Faina; Eisenthal, Avi; Maymon, Batia Bar-Shira

doi:10.1007/s004280100528

Cited by 23 publications

(14 citation statements)

References 28 publications

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“…34 Indeed, the autosomal homologue of DAZ, DAZL1, is expressed in testicular germ cell tumours. 35 In conclusion, although our data do not show an effect of the Y chromosome background in testicular cancer in a cohort of English patients, it is necessary to study other populations where the incidence of TC is rising dramatically and where a Y chromosome haplogroup has been found to be associated with reduced testicular function, such as in Denmark. 28 …”

Section: Resultsmentioning

confidence: 50%

Y chromosome haplotypes and testicular cancer in the English population

Quintana-Murci

2003

Journal of Medical Genetics

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Section: Resultsmentioning

confidence: 50%

Y chromosome haplotypes and testicular cancer in the English population

Quintana-Murci

2003

Journal of Medical Genetics

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“…However, JKT-1 shows no signal for BMP-2 either, also excluding this possibility. We further demonstrate that JKT-1 lacks expression of the germ cell markers DAZL (Lifschitz-Mercer et al 2002) BOULE, and VASA (Ezeh et al 2005), raising the question as to whether JKT-1 can be regarded as a GCT line at all. Hence, the data presented here suggest that JKT-1, although initially clustering to embryonal carcinomas, does not express a convincing set of markers indicative for GCTs and germ cells.…”

Section: Discussionmentioning

confidence: 94%

TCam-2 but not JKT-1 cells resemble seminoma in cell culture

et al. 2007

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Of all malignancies diagnosed in men between 17 and 45 years of age, 60% are germ cell tumors (GCT). GCT arise from carcinoma in situ cells, which are thought to originate from a transformed fetal germ cell, the gonocyte. Seminoma together with embryonal carcinoma represent the most frequent subtypes of GCT. However, the nature of the molecular pathways involved in seminoma formation remains elusive. Therefore, analysis of appropriate cell culture systems is an important prerequisite for further understanding of the etiology of this tumor entity. Although several cell lines for embryonal carcinoma have been established and analyzed, so far only two cell lines from seminoma patients have been reported. In the present study, we have analyzed these seminoma cell lines (TCam-2 and JKT-1) and compared the gene-expression profiles with those of normal tissue and of seminoma and embryonal carcinoma by using DNA Array technology. We have found that TCam-2 clusters with the group of classical seminoma, whereas JKT-1 clusters with the group of embryonal carcinoma. Using reverse transcription/polymerase chain reaction, Western blot, and immunohistochemistry, we have confirmed the seminoma-like nature of TCam-2, whereas JKT-1 lacks expression for most of the genes detectable in GCTs, thus making doubtful the germ cell nature of this cell line. The data represent the first genome-wide expression analysis of the two cell lines and comparison/clustering with subgroups of germ cell tumors. Only TCam-2 seems to represent a suitable in vitro model for seminoma.

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“…Cells expressed the Sertoli cell marker Gata4 [17] and were negative for Dazl1 [24] and Vasa [25] (germ cells), Oct4 and Nanog (spermatogonial stem cells, [26]) and Hsd3β6 (Leydig cells, [27]) (Figure S1B). …”

Section: Resultsmentioning

confidence: 99%

Reduced Immunogenicity of Induced Pluripotent Stem Cells Derived from Sertoli Cells

et al. 2014

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Sertoli cells constitute the structural framework in testis and provide an immune-privileged environment for germ cells. Induced pluripotent stem cells (iPS cells) resemble embryonic stem cells (ES cells) and are generated from somatic cells by expression of specific reprogramming transcription factors. Here, we used C57BL/6 (B6) Sertoli cells to generate iPS cells (Ser-iPS cells) and compared the immunogenicity of Ser-iPS cells with iPS cells derived from mouse embryonic fibroblast (MEF-iPS cells). Ser-iPS cells were injected into syngeneic mice to test for their in vivo immunogenicity in teratoma assay. Teratoma assay allows assessing in vivo immunogenicity of iPS cells and of their differentiated progeny simultaneously. We observed that early-passage Ser-iPS cells formed more teratomas with less immune cell infiltration and tissue damage and necrosis than MEF-iPS cells. Differentiating Ser-iPS cells in embryoid bodies (EBs) showed reduced T cell activation potential compared to MEF-iPS cells, which was similar to syngeneic ES cells. However, Ser-iPS cells lost their reduced immunogenicity in vivo after extended passaging in vitro and late-passage Ser-iPS cells exhibited an immunogenicity similar to MEF-iPS cells. These findings indicate that early-passage Ser-iPS cells retain some somatic memory of Sertoli cells that impacts on immunogenicity of iPS cells and iPS cell-derived cells in vivo and in vitro. Our data suggest that immune-privileged Sertoli cells might represent a preferred source for iPS cell generation, if it comes to the use of iPS cell-derived cells for transplantation.

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Localization of a specific germ cell marker, DAZL1, in testicular germ cell neoplasias

Cited by 23 publications

References 28 publications

Y chromosome haplotypes and testicular cancer in the English population

Y chromosome haplotypes and testicular cancer in the English population

TCam-2 but not JKT-1 cells resemble seminoma in cell culture

Reduced Immunogenicity of Induced Pluripotent Stem Cells Derived from Sertoli Cells

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