Localization of a Glycosphingolipid, Asialo GM1, in Rat Immunocytes1

Abstract

One of the TLMA (rat T-lymphocyte-macrophage-associated antigens) was characterized as asialo GM1, Gal(beta, 1-3)GalNAc(beta, 1-4)Gal(beta, 1-1)-Cer by two assay methods using monospecific anti-asialo GM1 antibody, immunocytotoxicity testing and immunostaining. Nylon column-enriched T lymphocytes (non-adherent cells) were highly cytolyzed by anti-asialo GM1 antiserum and complement as compared with adherent cells (non T cells). Forty % of peritoneal exudate macrophages and 20% of granulocytes were also cytolyzed by anti-asialo GM1 antiserum. On the other hand, 5% of thymic cells and 60-80% of lymph node T cells were immunostained by anti-asialo GM1 antibody. These asialo GM1-positive cells in thymus were found to be localized in the cortico-medulla junction. About 20% of macrophages and 2)% of granulocytes were also immunostained by anti-asialo GM1 F (ab')2 fragment. These results strongly support the previous conclusion that TLMA can be identified as asialo GM1. Asialo GM1 was universally expressed on a certain population of macrophages of various tissues, but not on all of the population. Alveolar macrophages were also asialo GM1-positive.

Toxoplasma gondiiTriggers Granulocyte-Dependent Cytokine-Mediated Lethal Shock ind-Galactosamine-Sensitized Mice

“…Our finding that depletion of asialo-GM-1-positive cells confers resistance also implicates NK cells in the cascade of events leading to death. Nevertheless, the latter result must be interpreted with caution, because this phenotypic marker has also been reported to be on subsets of cells of the monocyte-granulocyte lineage (50). Together, the results suggest granulocyte involvement in triggering a cascade of inflammatory cytokines, resulting in progressive NO accumulation and ultimately precipitating host death.…”

Natural Killer Cell Reduction and Uteroplacental Vasculopathy

“…This antiserum reduces NK cells in almost every organ in the body, including spleen 16,47 rather than just uNK cells. Moreover, anti-asialo GM1 also reacts with nonparenchymal liver cells, 48 T cells in mice 49 and rats, 50 as well as murine basophils 51 and monocytes 52 and rat granulocytes and macrophages. 50 The effect on various ILC subsets is poorly described.…”

“…Moreover, anti-asialo GM1 also reacts with nonparenchymal liver cells, 48 T cells in mice 49 and rats, 50 as well as murine basophils 51 and monocytes 52 and rat granulocytes and macrophages. 50 The effect on various ILC subsets is poorly described. Thus, we cannot conclude that the effects we observed were solely the result of NK or even uNK cell reduction.…”

Neutral glycosphingolipids of murine myeloma cells and helper, cytolytic, and suppressor T lymphocyte

“…In studies with anti-Gg4Cer (asialo-GMi) antisera, Gg4Cer was reported to be present on mature murine T cells (Stein et immunocytes of the rat (Momoi et al, 1982). Schwarting & Summers (1980) detected Gg4Cer in murine splenic T cells also by HPLC.…”