Localization of 31 porcine transcripts to the pig genome by SSRH radiation hybrid mapping

Abstract

We performed RH mapping of 38 transcripts including 7 previously mapped markers selected from full-length enriched cDNA libraries of pigs to determine their locations within the porcine genome and mapped them to 13 chromosomes. The chromosomes with the largest number of mapped transcripts were Sus scrofa chromosome (SSC) 1 with 6, and 5 transcripts were mapped to SSC6 and 9 each. The average retention frequency of the amplified products was 31%, ranging from 74% for SSC9 to 12% for SSC17. The evolutionary conservation of syntenic structures for the chromosomal regions linked to the 38 genes was confirmed using 86 previously mapped genes surrounding them, indicating that the general syntenic structures of chromosomes were conserved among cattle, humans and pigs except for the presence of additional chromosomal breakages in humans and bovine due to an increase in chromosome numbers. The mapping information of 31 new genes can be used to more finely analyze the pig genome or could be used as functional gene markers for porcine QTL mapping.