Localization and transport of indole-3-acetic acid during somatic embryogenesis in Coffea canephora

Márquez-López, Ruth E.; Pérez-Hernández, Cleyre; Kú-González, Ángela; Galáz-Ávalos, Rosa M.; Loyola‐Vargas, Víctor M.

doi:10.1007/s00709-017-1181-1

Cited by 43 publications

(34 citation statements)

References 52 publications

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“…It seems that not the initial level of IAA in explant tissue but rather a dynamic increase of the endogenous auxin content in response to the SE-induction factors might provide an indispensable signal for the embryogenic transition. Relevantly, a significant increase in the IAA content was reported to accompany early SE induction in different plants including carrot [48], sunflower [49], robusta coffee [50][51][52], alfalfa [53], pineapple guava [54], Arabidopsis [36], cotton [55] and Norway spruce [56]. Besides in vitro culture conditions, transgenic plants of Arabidopsis that produced somatic embryos in planta as a result of the overexpression of the LEAFY COTYLEDON2 (LEC2) gene were also found to accumulate IAA [57].…”

Section: Biosynthesis and Accumulation Of Auxin During Sementioning

confidence: 99%

Current Perspectives on the Auxin-Mediated Genetic Network that Controls the Induction of Somatic Embryogenesis in Plants

Wójcik

Wójcikowska

Gaj

2020

IJMS

100

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Auxin contributes to almost every aspect of plant development and metabolism as well as the transport and signalling of auxin-shaped plant growth and morphogenesis in response to endo- and exogenous signals including stress conditions. Consistently with the common belief that auxin is a central trigger of developmental changes in plants, the auxin treatment of explants was reported to be an indispensable inducer of somatic embryogenesis (SE) in a large number of plant species. Treating in vitro-cultured tissue with auxins (primarily 2,4-dichlorophenoxyacetic acid, which is a synthetic auxin-like plant growth regulator) results in the extensive reprogramming of the somatic cell transcriptome, which involves the modulation of numerous SE-associated transcription factor genes (TFs). A number of SE-modulated TFs that control auxin metabolism and signalling have been identified, and conversely, the regulators of the auxin-signalling pathway seem to control the SE-involved TFs. In turn, the different expression of the genes encoding the core components of the auxin-signalling pathway, the AUXIN/INDOLE-3-ACETIC ACIDs (Aux/IAAs) and AUXIN RESPONSE FACTORs (ARFs), was demonstrated to accompany SE induction. Thus, the extensive crosstalk between the hormones, in particular, auxin and the TFs, was revealed to play a central role in the SE-regulatory network. Accordingly, LEAFY COTYLEDON (LEC1 and LEC2), BABY BOOM (BBM), AGAMOUS-LIKE15 (AGL15) and WUSCHEL (WUS) were found to constitute the central part of the complex regulatory network that directs the somatic plant cell towards embryogenic development in response to auxin. The revealing picture shows a high degree of complexity of the regulatory relationships between the TFs of the SE-regulatory network, which involve direct and indirect interactions and regulatory feedback loops. This review examines the recent advances in studies on the auxin-controlled genetic network, which is involved in the mechanism of SE induction and focuses on the complex regulatory relationships between the down- and up-stream targets of the SE-regulatory TFs. In particular, the outcomes from investigations on Arabidopsis, which became a model plant in research on genetic control of SE, are presented.

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Section: Biosynthesis and Accumulation Of Auxin During Sementioning

confidence: 99%

Current Perspectives on the Auxin-Mediated Genetic Network that Controls the Induction of Somatic Embryogenesis in Plants

Wójcik

Wójcikowska

Gaj

2020

IJMS

100

View full text Add to dashboard Cite

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“…The first wave of expressing genes comprises transcription factors (TFs) such as AGL15 , LEC2 , LEC1 , BBM , MAD -box, and WUS ( Yang et al, 2012 ; Indoliya et al, 2016 ; Cao et al, 2017 ; Jamaluddin et al, 2017 ; Magnani et al, 2017 ). Then a second wave of transcription involves genes coding for enzymes involved in the homeostasis of auxins and cytokinins, as well as other PGRs ( Ayil-Gutiérrez et al, 2013 ; Márquez-López et al, 2018 ).…”

Section: Introductionmentioning

confidence: 99%

Advanced Proteomic Approaches to Elucidate Somatic Embryogenesis

Aguilar-Hernández

Loyola‐Vargas

2018

Front. Plant Sci.

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Somatic embryogenesis (SE) is a cell differentiation process by which a somatic cell changes its genetic program and develops into an embryonic cell. Investigating this process with various explant sources in vitro has allowed us to trace somatic embryo development from germination to plantlets and has led to the generation of new technologies, including genetic transformation, endangered species conservation, and synthetic seed production. A transcriptome data comparison from different stages of the developing somatic embryo has revealed a complex network controlling the somatic cell’s fate, suggesting that an interconnected network acts at the protein level. Here, we discuss the current progress on SE using proteomic-based data, focusing on changing patterns of proteins during the establishment of the somatic embryo. Despite the advanced proteomic approaches available so far, deciphering how the somatic embryo is induced is still in its infancy. The new proteomics techniques that lead to the quantification of proteins with different abundances during the induction of SE are opening this area of study for the first time. These quantitative differences can elucidate the different pathways involved in SE induction. We envisage that the application of these proteomic technologies can be pivotal to identifying proteins critical to the process of SE, demonstrating the cellular localization, posttranslational modifications, and turnover protein events required to switch from a somatic cell to a somatic embryo cell and providing new insights into the molecular mechanisms underlying SE. This work will help to develop biotechnological strategies for mass production of quality crop material.

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“…of 24 were homologous to sequences in known protein libraries or identified as the same protein domain were retained.…”

mentioning

confidence: 99%

Full-Length Transcriptome Analysis of the ABCB, PIN/PIN-LIKES, and AUX/LAX Families Involved in Somatic Embryogenesis of Lilium pumilum DC. Fisch.

Song

Wang

Ren

et al. 2020

IJMS

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Plant cell totipotency is one of the 25 major topics in current scientific research, and somatic embryos are good experimental material for studying cell totipotency. Polar auxin transport plays an important regulatory role in somatic embryogenesis (SE). However, little is known about the auxin transport genes and their regulatory mechanisms in Lilium SE. In this study, we applied single-molecule real-time (SMRT) sequencing to Lilium pumilum DC. Fisch. for the first time and obtained a total of 119,649 transcripts, of which 14 encoded auxin transport genes. Correlation analyses between somatic embryo induction and gene expression under different treatments revealed that auxin transport genes, especially ATP-binding cassette (ABC) transporter B family member 21 (ABCB21) and PIN-FORMED (PIN) LIKES 7 (PILS7), may be key players in SE, and the necessary duration of picloram (PIC) treatment to induce SE is as short as 3 days. Our research provides valuable genetic information on Lilium pumilum, elucidating the candidate auxin transport genes involved in SE and their influencing factors. This study lays a foundation for elucidating the regulatory mechanism of auxin transport in SE.

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Localization and transport of indole-3-acetic acid during somatic embryogenesis in Coffea canephora

Cited by 43 publications

References 52 publications

Current Perspectives on the Auxin-Mediated Genetic Network that Controls the Induction of Somatic Embryogenesis in Plants

Current Perspectives on the Auxin-Mediated Genetic Network that Controls the Induction of Somatic Embryogenesis in Plants

Advanced Proteomic Approaches to Elucidate Somatic Embryogenesis

Full-Length Transcriptome Analysis of the ABCB, PIN/PIN-LIKES, and AUX/LAX Families Involved in Somatic Embryogenesis of Lilium pumilum DC. Fisch.

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