Localization and Quantification of Drugs in Animal Tissues by Use of Desorption Electrospray Ionization Mass Spectrometry Imaging

Vismeh, Ramin; Waldon, Daniel J.; Teffera, Yohannes; Zhao, Zhiyang

doi:10.1021/ac3011654

Cited by 97 publications

(95 citation statements)

References 34 publications

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“…For the determination of analytes in intact tissues using imaging DESI-MS, previous efforts have focused on relative quantification [36]. However, absolute quantitation can also be done [37]. These experiments require the careful selection of internal standards and their homogeneous transfer to the sample.…”

Section: Resultsmentioning

confidence: 99%

Analysis of Metabolic Changes in Plant Pathosystems by Imprint Imaging DESI-MS

Tata

Pérez

Hamid

et al. 2014

J. Am. Soc. Mass Spectrom.

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Abstract. The response of plants to microbial pathogens is based on the production of secondary metabolites. The complexity of plant-pathogen interactions makes their understanding a challenging task for metabolomic studies requiring powerful analytical approaches. In this paper, the ability of ambient mass spectrometry to provide a snapshot of plant metabolic response to pathogen invasion was tested. The fluctuations of glycoalkaloids present in sprouted potatoes infected by the phytopathogen Pythium ultimum were monitored by imprint imaging desorption electrospray ionization mass spectrometry (DESI-MS). After 8 d from the inoculation, a decrease of the relative abundance of potato glycoalkaloids α-solanine (m/z 706) and α-chaconine (m/z 722) was observed, whereas the relative intensity of solanidine (m/z 398), solasodenone (m/z 412), solanaviol (m/z 430), solasodiene (m/z 396), solaspiralidine (m/z 428), γ-solanine/γ-chaconine (m/z 560) , β-solanine (m/z 706), and β-chaconine (m/z 722) increased. The progression of the disease, expressed by the development of brown necrotic lesions on the potato, led to the further decrease of all the glycoalkaloid metabolites. Therefore, the applicability of imprint imaging DESI-MS in studying the plant metabolic changes in a simple pathosystem was demonstrated with minimal sample preparation.

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Section: Resultsmentioning

confidence: 99%

Analysis of Metabolic Changes in Plant Pathosystems by Imprint Imaging DESI-MS

Tata

Pérez

Hamid

et al. 2014

J. Am. Soc. Mass Spectrom.

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“…The relative standard deviation (RSD)% ranged from 3 to 13% when ESI was used in comparison to the higher RSD% values obtained from DESI (10-31%), suggesting its suitability as a semiquantitative application (73). However, absolute quantification was performed using DESI-MS (74,75), albeit usually less sensitive than its ESI-LC-MS. In a recent study, five MS-based high-throughput quantification methods were developed to quantify the drug delivery agent, gemini surfactant, within cells.…”

Section: Desorption Electrospray Ionizationmentioning

confidence: 97%

Mass Spectrometry, Review of the Basics: Ionization

Awad

Khamis

El‐Aneed

2014

Applied Spectroscopy Reviews

121

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Mass spectrometry (MS) has become an integral tool in life sciences. The first step in MS analysis is ion formation (ionization). Many ionization methods currently exist; electrospray ionization (ESI) and matrix-assisted laser desorption ionization (MALDI) are the most commonly used. ESI relies on the formation of charged droplets releasing ions from the surface (ion evaporation model) or via complete solvent evaporation (charge residual model). MALDI ionization, however, is facilitated via laser energy and the use of a matrix. Despite wide use, ESI cannot efficiently ionize nonpolar compounds. Atmospheric pressure chemical ionization (APCI) and atmospheric pressure photo ionization (APPI) are better suited for such tasks. APPI requires photon energy and a dopant, whereas APCI is similar to chemical ionization. In 2004, ambient MS was introduced in which ionization occurs at the sample in its native form. Desorption electrospray ionization (DESI) and direct analysis in real time (DART) are the most widely used methods. In this mini-review, we provide an overview of the main ionization methods and the mechanisms of ion formation. This article is educational and intended for students/researchers who are not very familiar with MS and would like to learn the basics; it is not for MS experts.

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“…Desorption electrospray ionization (DESI) is an ambient ionization technique for mass spectrometry [22,23]. No matrix and complicated sample preparation process are needed in DESI-MS experiments.…”

Section: Electronic Supplementary Materialsmentioning

confidence: 99%

Screening of the Binding of Small Molecules to Proteins by Desorption Electrospray Ionization Mass Spectrometry Combined with Protein Microarray

Yao

Wang

Zhang

et al. 2015

J. Am. Soc. Mass Spectrom.

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Abstract. The interaction between bioactive small molecule ligands and proteins is one of the important research areas in proteomics. Herein, a simple and rapid method is established to screen small ligands that bind to proteins. We designed an agarose slide to immobilize different proteins. The protein microarrays were allowed to interact with different small ligands, and after washing, the microarrays were screened by desorption electrospray ionization mass spectrometry (DESI MS). This method can be applied to screen specific protein binding ligands and was shown for seven proteins and 34 known ligands for these proteins. In addition, a high-throughput screening was achieved, with the analysis requiring approximately 4 s for one sample spot. We then applied this method to determine the binding between the important protein matrix metalloproteinase-9 (MMP-9) and 88 small compounds. The molecular docking results confirmed the MS results, demonstrating that this method is suitable for the rapid and accurate screening of ligands binding to proteins.

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Localization and Quantification of Drugs in Animal Tissues by Use of Desorption Electrospray Ionization Mass Spectrometry Imaging

Cited by 97 publications

References 34 publications

Analysis of Metabolic Changes in Plant Pathosystems by Imprint Imaging DESI-MS

Analysis of Metabolic Changes in Plant Pathosystems by Imprint Imaging DESI-MS

Mass Spectrometry, Review of the Basics: Ionization

Screening of the Binding of Small Molecules to Proteins by Desorption Electrospray Ionization Mass Spectrometry Combined with Protein Microarray

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