Localization and functional analysis of HmgB3p, a novel protein containing high-mobility-group-box domain from Tetrahymena thermophila

Xu, Jing; Tian, Huaru; Liu, Xingyin; Wang, Wei; Liang, Aihua

doi:10.1016/j.gene.2013.05.006

Cited by 10 publications

(11 citation statements)

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“…Tetrahymena Functional Genomics Database (TetraFGD, http://tfgd.ihb.ac.cn ) shows that MLH1 has a similar expression profile as HMGB3 [ 21 , 27 ]. Furthermore, HmgB3 and Mlh1 contain similar numbers of charged and hydrophobic amino acids, and share the conserved HMG box domain [ 19 ]. Thus, Mlh1 and HmgB3p could have similar functions in Tetrahymena .…”

Section: Resultsmentioning

confidence: 99%

“…In Tetrahymena , macronuclear Histone H1 is highly phosphorylated at serine or threonine residues located in a cdc2 kinase consensus motif [ 28 ]. HmgB3 is also phosphorylated at 7 different residues [ 19 ]. Although phosphoproteomics analysis of Tetrahymena showed that Mlh1 has no phosphorylated modification,Wu M. et al showed that Mlh1 contains 16 protein kinase A phosphorylation sites [ 16 ].…”

Section: Resultsmentioning

confidence: 99%

“…The fragments were introduced into the CU428 and B2086 strains using biolistic particle transformation system GJ-1000 (SCIENTZ, China). Tansformants were selected on the basis of resistance to paromomycin and identified by PCR using the MTT1 -FW/ MTT1 -RV primer set, as described previously [ 19 ].…”

Section: Methodsmentioning

confidence: 99%

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Micronucleus-specific histone H1 is required for micronuclear chromosome integrity in Tetrahymena thermophila

Qiao

Tao

et al. 2017

PLoS ONE

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Histone H1 molecules play a key role in establishing and maintaining higher order chromatin structures. They can bind to linker DNA entering and exiting the nucleosome and regulate transcriptional activity. Tetrahymena thermophila has two histone H1, namely, macronuclear histone H1 and micronuclear histone H1 (Mlh1). Mlh1 is specifically localized at micronuclei during growth and starvation stages. Moreover, Mlh1 is localized around micronuclei and forms a specific structure during the conjugation stage. It co-localizes partially with spindle apparatus during micronuclear meiosis. Analysis of MLH1 knock-out revealed that Mlh1 was required for the micronuclear integrity and development during conjugation stage. Overexpression of Mlh1 led to abnormal conjugation progression. RT-PCR analysis indicated that the expression level of HMGB3 increased in ΔMLH1 strains, while the expression level of MLH1 increased in ΔHMGB3 cells during conjugation. These results indicate that micronuclear integrity and sexual development require normal expression level of Mlh1 and that HmgB3 and Mlh1 may functionally compensate each other in regulating micronuclear structure in T. thermophila.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

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Micronucleus-specific histone H1 is required for micronuclear chromosome integrity in Tetrahymena thermophila

Qiao

Tao

et al. 2017

PLoS ONE

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“…Our experiments showed that the selected pronucleus also undergoes acetylation at sites other than at H3K56; for example, H3K18ac and H3K27ac (Figure 9—figure supplement 1) are strongly enriched in euchromatin (Wang et al, 2008; Tie et al, 2009). Further, a protein containing a high mobility group (HMG) box domain, which decreases the compactness of the chromatin fiber (Agresti and Bianchi, 2003; Catez et al, 2004), is abundantly expressed in the selected pronucleus (Xu et al, 2013). Together, these findings suggest that repair of programmed PM-DSBs in the haploid germline allows changes in the epigenetic landscape required to produce mature gametes (Figure 11).…”

Section: Discussionmentioning

confidence: 99%

Post-meiotic DNA double-strand breaks occur in Tetrahymena, and require Topoisomerase II and Spo11

Akematsu

Fukuda

Garg

et al. 2017

eLife

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Based on observations of markers for DNA lesions, such as phosphorylated histone H2AX (γH2AX) and open DNA ends, it has been suggested that post-meiotic DNA double-strand breaks (PM-DSBs) enable chromatin remodeling during animal spermiogenesis. However, the existence of PM-DSBs is unconfirmed, and the mechanism responsible for their formation is unclear. Here, we report the first direct observation of programmed PM-DSBs via the electrophoretic separation of DSB-generated DNA fragments in the ciliate Tetrahymena thermophila. These PM-DSBs are accompanied by switching from a heterochromatic to euchromatic chromatin structure in the haploid pronucleus. Both a topoisomerase II paralog with exclusive pronuclear expression and Spo11 are prerequisites for PM-DSB induction. Reduced PM-DSB induction blocks euchromatin formation, characterized by histone H3K56 acetylation, leading to a failure in gametic nuclei production. We propose that PM-DSBs are responsible for histone replacement during the reprogramming of generative to undifferentiated progeny nuclei.DOI: http://dx.doi.org/10.7554/eLife.26176.001

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“…Exposure to Cd in the gills and hepatopancreas of oyster showed activation of ABCB1 protein but no significant changes to its mRNA, suggesting posttranscriptional regulation of ABCB1 [57]. [58]. For physiological and biochemical analyses, cells were transferred to 10 mL of SPP medium with the same initial concentration at 0.7 × 10 5 mL − 1 .…”

Section: Characterization Of the Sequenced Illumina Librariesmentioning

confidence: 99%

Comparative Transcriptome Analysis Uncovers Roles of Hydrogen Sulfide for Alleviating Cadmium Toxicity in Tetrahymena Thermophila

Xu²,

et al. 2020

Preprint

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BackgroundCadmium (Cd) is a nonessential heavy metal with potentially deleterious effects on different organisms. The organisms have evolved sophisticated defense system to alleviate heavy metal toxicity. Hydrogen sulfide (H2S) effectively alleviates heavy metal toxicity in plants. However, the function of H2S for alleviating heavy metal toxicity in aquatic organisms remains less clear. Tetrahymena thermophila is an important model organism to evaluate toxic contaminants in an aquatic environment. In this study, the molecular roles of exogenously H2S application were explored by RNA sequencing under Cd stress in T. thermophila.ResultsThe exposure of 30 µM Cd resulted in T. thermophila growth inhibition, cell nigrescence, and malondialdehyde (MDA) content considerably increase. However, exogenous H2S (70 µM) significantly alleviated the Cd-induced toxicity and improved antioxidant system. Comparative transcriptome analysis showed that the expression levels of 9152 genes changed under Cd stress (4658 upregulated and 4494 downregulated). However, only 1359 genes were differentially expressed with H2S treatment under Cd stress (1087 upregulated and 272 downregulated). The functional categories of the differentially expressed genes (DEGs) by gene ontology (GO) revealed that the transcripts involved in the oxidation–reduction process, oxidoreductase activity, glutathione peroxidase activity, and cell redox homeostasis were the considerable enrichments between Cd stress and H2S treatment under Cd stress. Kyoto Encyclopedia of Genes and Genomes (KEGG) indicated that the carbon metabolism, glutathione metabolism, metabolism of xenobiotics by cytochrome P450, and ABC transporters were significantly differentially expressed components between Cd stress and H2S treatment under Cd stress in T. thermophila. The relative expression levels of six DEGs were further confirmed through quantitative real-time polymerase chain reaction (qRT-PCR).ConclusionH2S alleviated Cd stress mainly through increasing oxidation resistance, enhancing detoxification, and regulation of transport in free-living unicellular T. thermophila. These findings will expand our understanding for H2S functions in the freshwater protozoa.

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Localization and functional analysis of HmgB3p, a novel protein containing high-mobility-group-box domain from Tetrahymena thermophila

Cited by 10 publications

References 58 publications

Micronucleus-specific histone H1 is required for micronuclear chromosome integrity in Tetrahymena thermophila

Micronucleus-specific histone H1 is required for micronuclear chromosome integrity in Tetrahymena thermophila

Post-meiotic DNA double-strand breaks occur in Tetrahymena, and require Topoisomerase II and Spo11

Comparative Transcriptome Analysis Uncovers Roles of Hydrogen Sulfide for Alleviating Cadmium Toxicity in Tetrahymena Thermophila

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