Localization and Expression of Peroxiredoxin II in the Mouse Ovary, Oviduct, Uterus, and Preimplantation Embryo

Abstract

Peroxiredoxin (Prx) II belongs to a recently discovered family of peroxidases that play important roles in antioxidation and signal transduction. In this study, we aimed to study the localization and expression of Prx II in the mouse ovary, oviduct, and uterus, and preimplantation embryos. Immunohistochemical staining analysis showed that, in the ovary, Prx II was expressed in the oocyte cytoplasm of the primary follicle, the secondary follicle, and the premature follicle; Prx II was expressed in germinal vesicle-intact oocytes (GV oocytes) and metaphase II eggs (MII eggs), as well as at various stages in early embryos. Reverse transcription polymerase chain reaction (RT-PCR) results indicated that the Prx II mRNA was expressed at a high level in GV eggs, slightly lower levels in MII eggs, and had no detectable expression in four-cell embryos and early blastocysts. In the oviduct, Prx II was expressed in the epithelia, while in the uterus Prx II was mainly distributed in the endometrial stroma. Taken together, our results suggest that Prx II plays a key antioxidation role in the maturation of oocytes and development of early embryos, thus providing crucial experimental evidence for further exploring the function of Prx II in the development of oocytes and preimplantation embryos. Anat Rec,[293][294][295][296][297]

Effect of peroxiredoxin II on the quality and mitochondrial activity of pre-implantation bovine embryos

“…The mRNA transcription and protein translation of PRDX II begin in oocytes in primary follicles and continue until the preovulatory stage (Wang et al, 2010). PRDX II expression is dynamically regulated at the translational level; therefore, it probably belongs to the maternal mRNA (Wang et al, 2010). Moreover, PRDX II is detectable in germinal vesicle-stage oocytes, metaphase II-stage oocytes, and pre-implantation embryos by immunohistochemical analysis (Wang et al, 2010).…”

“…PRDX II expression is dynamically regulated at the translational level; therefore, it probably belongs to the maternal mRNA (Wang et al, 2010). Moreover, PRDX II is detectable in germinal vesicle-stage oocytes, metaphase II-stage oocytes, and pre-implantation embryos by immunohistochemical analysis (Wang et al, 2010). PRDX II protein may provide a suitable microenvironment for oocyte growth in the ovary via eliminating ROS generated in metabolic processes, and may also promote oocyte growth via suppressing oocyte death (Wang et al, 2010).…”

“…Moreover, PRDX II is detectable in germinal vesicle-stage oocytes, metaphase II-stage oocytes, and pre-implantation embryos by immunohistochemical analysis (Wang et al, 2010). PRDX II protein may provide a suitable microenvironment for oocyte growth in the ovary via eliminating ROS generated in metabolic processes, and may also promote oocyte growth via suppressing oocyte death (Wang et al, 2010). The research have been demonstrated that the expression of PRDX II in the uterus and oviduct of mouse, play a crucial role for eliminating endogenous and exogenous ROS, creating a suitable microenvironment for egg fertilization, preimplantation and postimplantation of embryos (Wang et al, 2010).…”

“…In this study, we investigated the effect of exogenous PRDX II during in vitro maturation, an antioxidant that has oxidoreductase activity, on embryo development in vitro. The mRNA transcription and protein translation of PRDX II begin in oocytes in primary follicles and continue until the preovulatory stage (Wang et al, 2010). PRDX II expression is dynamically regulated at the translational level; therefore, it probably belongs to the maternal mRNA (Wang et al, 2010).…”

“…PRDX II protein may provide a suitable microenvironment for oocyte growth in the ovary via eliminating ROS generated in metabolic processes, and may also promote oocyte growth via suppressing oocyte death (Wang et al, 2010). The research have been demonstrated that the expression of PRDX II in the uterus and oviduct of mouse, play a crucial role for eliminating endogenous and exogenous ROS, creating a suitable microenvironment for egg fertilization, preimplantation and postimplantation of embryos (Wang et al, 2010). Interestingly, our data showed reduction in ROS level in oocytes and their corresponding embryos of the treated group with PRDX II compared to control.…”