Localization and distribution of hopanoids in membrane systems of the cyanobacterium<i>Synechocystis</i>PCC 6714

JÃ1⁄4rgens, Uwe J.; Simonin, Pascale; Rohmer, Michel

doi:10.1111/j.1574-6968.1992.tb05275.x

Cited by 12 publications

References 9 publications

(3 reference statements)

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C₃₅ Hopanoid Side Chain Biosynthesis: Reduction of Ribosylhopane into Bacteriohopanetetrol by a Cell‐Free System Derived from Methylobacterium organophilum

et al. 2015

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The major bacterial triterpenoids of the hopane series each consist of a C30 triterpene hopane moiety and an additional nonterpene C5 side chain derived from D-ribose and linked through its C-5 carbon atom to the hopane side chain. Bacteriohopanetetrol and aminobacteriohopanetriol are the most common representatives of this natural product series, adenosylhopane and ribosylhopane being putative precursors. Deuterium-labelled ribosylhopane was obtained by hemisynthesis and converted into deuterium-labelled bacteriohopanetetrol in the presence of NADPH, thus giving evidence of this as yet unknown precursor-to-product relationship in the bacterial hopanoid metabolic pathway.

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C₃₅ Hopanoid Side Chain Biosynthesis: Reduction of Ribosylhopane into Bacteriohopanetetrol by a Cell‐Free System Derived from Methylobacterium organophilum

et al. 2015

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Biosynthesis of 2-methylbacteriohopanepolyols by an anoxygenic phototroph

Rashby

Sessions

Summons

et al. 2007

Proc. Natl. Acad. Sci. U.S.A.

254

185

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The RND-family transporter, HpnN, is required for hopanoid localization to the outer membrane of Rhodopseudomonas palustris TIE-1

Doughty

Coleman²,

Hunter

et al. 2011

Proc. Natl. Acad. Sci. U.S.A.

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Rhodopseudomonas palustris TIE-1 is a Gram-negative bacterium that produces structurally diverse hopanoid lipids that are similar to eukaryotic steroids. Its genome encodes several homologues to proteins involved in eukaryotic steroid trafficking. In this study, we explored the possibility that two of these proteins are involved in intracellular hopanoid transport. R. palustris has a sophisticated membrane system comprising outer, cytoplasmic, and inner cytoplasmic membranes. It also divides asymmetrically, producing a mother and swarmer cell. We deleted genes encoding two putative hopanoid transporters that belong to the resistance–nodulation–cell division superfamily. Phenotypic analyses revealed that one of these putative transporters (HpnN) is essential for the movement of hopanoids from the cytoplasmic to the outer membrane, whereas the other (Rpal_4267) plays a minor role. C 30 hopanoids, such as diploptene, are evenly distributed between mother and swarmer cells, whereas hpnN is required for the C 35 hopanoid, bacteriohopanetetrol, to remain localized to the mother cell type. Mutant cells lacking HpnN grow like the WT at 30 °C but slower at 38 °C. Following cell division at 38 °C, the ΔhpnN cells remain connected by their cell wall, forming long filaments. This phenotype may be attributed to hopanoid mislocalization because a double mutant deficient in both hopanoid biosynthesis and transport does not form filaments. However, the lack of hopanoids severely compromises cell growth at higher temperatures more generally. Because hopanoid mutants only manifest a strong phenotype under certain conditions, R. palustris is an attractive model organism in which to study their transport and function.

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Localization and distribution of hopanoids in membrane systems of the cyanobacteriumSynechocystisPCC 6714

Cited by 12 publications

References 9 publications

C₃₅ Hopanoid Side Chain Biosynthesis: Reduction of Ribosylhopane into Bacteriohopanetetrol by a Cell‐Free System Derived from Methylobacterium organophilum

C₃₅ Hopanoid Side Chain Biosynthesis: Reduction of Ribosylhopane into Bacteriohopanetetrol by a Cell‐Free System Derived from Methylobacterium organophilum

Biosynthesis of 2-methylbacteriohopanepolyols by an anoxygenic phototroph

The RND-family transporter, HpnN, is required for hopanoid localization to the outer membrane of Rhodopseudomonas palustris TIE-1

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Localization and distribution of hopanoids in membrane systems of the cyanobacteriumSynechocystisPCC 6714

Cited by 12 publications

References 9 publications

C35 Hopanoid Side Chain Biosynthesis: Reduction of Ribosylhopane into Bacteriohopanetetrol by a Cell‐Free System Derived from Methylobacterium organophilum

C35 Hopanoid Side Chain Biosynthesis: Reduction of Ribosylhopane into Bacteriohopanetetrol by a Cell‐Free System Derived from Methylobacterium organophilum

Biosynthesis of 2-methylbacteriohopanepolyols by an anoxygenic phototroph

The RND-family transporter, HpnN, is required for hopanoid localization to the outer membrane of Rhodopseudomonas palustris TIE-1

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C₃₅ Hopanoid Side Chain Biosynthesis: Reduction of Ribosylhopane into Bacteriohopanetetrol by a Cell‐Free System Derived from Methylobacterium organophilum

C₃₅ Hopanoid Side Chain Biosynthesis: Reduction of Ribosylhopane into Bacteriohopanetetrol by a Cell‐Free System Derived from Methylobacterium organophilum