Localization and characterization of antigen‐presenting dendritic cells in the gastric mucosa of murine and human autoimmune gastritis

Ninomiya,; Matsui, Tomomi; Akbar,; Murakami,; Onji,

doi:10.1046/j.1365-2362.2000.00629.x

Cited by 13 publications

(12 citation statements)

References 23 publications

(55 reference statements)

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“…For humans, only one report has described the presence of a DC subpopulation in an H. pyloripositive MALT lymphoma (40). In the present study, there were no DCs in the gastric mucosa of normal mice, as has been suggested by other workers (31), and only a few myeloid and lymphoid DCs were present in the bottom portion of the lamina propria and in the submucosal area of the gastric mucosa of nTx mice that were not infected with H. pylori. The most noteworthy finding in this study was that in contrast to the noninfected nTx mice, a large number of myeloid DCs were diffusely infiltrated throughout the lamina propria in the H. pylori-infected nTx mouse stomachs, although the degree of lymphoid DC infiltration was not changed by H. pylori infection.…”

Section: Vol 71 2003 Dcs In H Pylori-induced Follicular Gastritis supporting

confidence: 85%

Involvement of Myeloid Dendritic Cells in the Development of Gastric Secondary Lymphoid Follicles inHelicobacter pylori-Infected Neonatally Thymectomized BALB/c Mice

Nishi¹,

Okazaki²,

Kawasaki³

et al. 2003

Infect Immun

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We previously described an animal model of Helicobacter pylori-induced follicular gastritis in neonatally thymectomized (nTx) mice. However, it is still not clear whether antigen-presenting dendritic cells (DCs) in the stomach have a role in the development of secondary follicles in H. pylori-infected nTx mice. We investigated the distribution of DC subsets using this model and examined their roles. To identify lymphoid and myeloid DCs, sections were stained with anti-CD11c (pan-DC marker) in combination with anti-CD8␣ (lymphoid DC marker) or anti-CD11b (myeloid DC marker) and were examined with a confocal microscope. Expression of macrophage inflammatory protein 3␣ (MIP-3␣), which chemoattracts immature DCs, was analyzed by realtime PCR and immunohistochemistry. Follicular dendritic cells (FDCs) were stained with anti-SKY28 antibodies. In noninfected nTx mice, a few myeloid and lymphoid DCs were observed in the bottom portion of the lamina propria, whereas in H. pylori-infected nTx mice, there was an increased influx of myeloid DCs throughout the lamina propria. FDC staining was also observed in the stomachs of members of the infected group. MIP-3␣ gene expression was upregulated in the infected nTx group, and the immunohistochemistry analysis revealed MIP-3␣-positive epithelial cells. These data suggest that H. pylori infection upregulates MIP-3␣ gene expression in gastric epithelial cells and induces an influx of myeloid DCs in the lamina propria of the gastric mucosa in nTx mice. Myeloid DCs and FDCs might contribute to the development of gastric secondary lymphoid follicles in H. pylori-infected nTx mice.Chronic Helicobacter pylori infection induces two distinct types of gastritis, chronic atrophic gastritis and nonatrophic gastritis (4). Nonatrophic gastritis is thought to be a predisposing factor for the development of mucosa-associated lymphoid tissue (MALT) lymphoma (8, 50). The organized structure of the secondary lymphoid tissues is believed to support proper regulation of activation and maturation of the antigen-responsive lymphoid cells (23,24). Although there is a substantial amount of data on the cellular elements that comprise the lymphoid and nonlymphoid components of the secondary lymphatic organs, little is known about the kinetics and roles of dendritic cells (DCs) that establish and maintain the proper organization of the secondary lymphoid tissues.DCs are immune regulatory cells that not only secrete chemokines and cytokines but also present antigens for T cells (39,42,43). In the intestine, DCs are constitutively present in the Peyer's patches, lamina propria, and mesenteric lymph nodes and have an essential role in the uptake of luminar bacterial antigens (37). Because normal gastric mucosa has no mucosaassociated lymphoid system, very little is known about the role of DCs in the mucosal immune system of the stomach, especially their role in H. pylori-induced chronic follicular gastritis.In previous studies (33, 46), it was demonstrated that H. pylori infection of BALB/c mice that were th...

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Section: Vol 71 2003 Dcs In H Pylori-induced Follicular Gastritis supporting

confidence: 85%

Involvement of Myeloid Dendritic Cells in the Development of Gastric Secondary Lymphoid Follicles inHelicobacter pylori-Infected Neonatally Thymectomized BALB/c Mice

Nishi¹,

Okazaki²,

Kawasaki³

et al. 2003

Infect Immun

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“…Time course studies in PC-GMCSF transgenic mice indicate that macrophages and dendritic cells precede the influx of CD4 T cells and B cells, supporting the critical role of antigen-presenting cells in the early phase of the autoimmune response (47). A role for dendritic cells has recently been implicated in human autoimmune gastritis (49).…”

Section: Environmentalmentioning

confidence: 85%

Animal Models of Human Disease: Experimental Autoimmune Gastritis—A Model for Autoimmune Gastritis and Pernicious Anemia

Alderuccio

Sentry

Marshall

et al. 2002

Clinical Immunology

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“…CCL19 and CCL21 are expressed in the T-cell zone of secondary lymphoid organs, where they exert potential chemotactic action for mature DCs through the interaction with CCR7, promoting immune and inflammatory processes [16, 17,24]. In fact, DCs with the mature phenotype have been identified in gastric mucosa in humans and mice with autoimmune gastritis [25], suggesting that they might contribute to gastric inflammation. A recent in vitro study has shown that DCs express CCR7 and migrate in response to CCL19 after exposure to H. pylori [24].…”

Section: Discussionmentioning

confidence: 99%

Enhanced expression of CCL20 in human Helicobacter pylori-associated gastritis

Yoshida

Isomoto

Hisatsune

et al. 2009

Clinical Immunology

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CC chemokine ligand 20 (CCL20) attracts CC chemokine receptor 6 (CCR6)-expressing cells. Using endoscopic biopsies taken from the gastric antrum of 42 subjects infected with H. pylori and 42 uninfected subjects, mucosal CCL20 mRNA and protein levels were measured by real-time polymerase chain reaction and enzyme-linked immunosorbent assay, respectively. CCL19 mRNA and protein levels, as well as CCL21 mRNA levels, were also measured. The CCL20 mRNA and protein levels were significantly elevated in H. pylori-positive patients and substantially decreased after successful eradication. CCL19 and CCL21 expression levels were comparable in the H. pylori-infected and the uninfected groups. The CCL20 concentrations correlated with the degree of chronic gastritis. Immunohistochemistry and the in vitro infection assay showed that CCL20 was principally produced by the gastric epithelium. CCR6-expressing cells, including CD45RO+ memory T lymphocytes and fascin+-CD1a+ immature dendritic cells, infiltrated close to the CCL20-expressing epithelial cells. The CCL20/CCR6 interaction may be involved in the development of H. pylori-associated gastritis.

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Localization and characterization of antigen‐presenting dendritic cells in the gastric mucosa of murine and human autoimmune gastritis

Cited by 13 publications

References 23 publications

Involvement of Myeloid Dendritic Cells in the Development of Gastric Secondary Lymphoid Follicles inHelicobacter pylori-Infected Neonatally Thymectomized BALB/c Mice

Involvement of Myeloid Dendritic Cells in the Development of Gastric Secondary Lymphoid Follicles inHelicobacter pylori-Infected Neonatally Thymectomized BALB/c Mice

Animal Models of Human Disease: Experimental Autoimmune Gastritis—A Model for Autoimmune Gastritis and Pernicious Anemia

Enhanced expression of CCL20 in human Helicobacter pylori-associated gastritis

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