Localization and analysis of natriuretic peptide receptors in the gills of the toadfish, Opsanus beta (teleostei)

Donald, John A.; Toop, Tes; Evans, David H.

doi:10.1152/ajpregu.1994.267.6.r1437

Cited by 14 publications

(14 citation statements)

References 22 publications

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“…Despite technical variations, the differing responses among fishes may be due to a species difference. A radioligand-binding assay showed that ANP receptors exist in the gills, but the predominant receptors are clearancetype NPR-C in eels (Sakaguchi et al, 1993) and toadfish (Donald et al, 1994). Further, Mishina and Takei (1997) showed that the NPR-A type is not detectable in the gills of SW eels as shown by the absence of cGMP accumulation after addition of ANP to isolated gill cells.…”

Section: Discussionmentioning

confidence: 99%

Relative Potency of Three Homologous Natriuretic Peptides (ANP, CNP and VNP) in Eel Osmoregulation

Tsukada

Takei

2001

Zoological Science

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-Atrial natriuretic peptide (ANP) decreases plasma Na + concentration and promtes seawater (SW) adaptation in eels. The hyponatremia may most probably be caused by increased branchial extrusion of Na + , but the mechanism has not been determined yet. The present study examined initially the effects of ANP on branchial Na + efflux in vivo using isotopic 22 Na. However, the efflux rate was not altered by infusion of a hyponatremic dose of ANP (5 pmol·kg). Therefore, we sought to examine whether the ANP-mediated hyponatremia is caused by a decrease in the uptake of Na + from the environment.Since a decrease in drinking was highly correlated with a degree of hyponatremia, conscious SW eels were infused with dilute SW into the stomach at a normal drinking rate to offset the antidipsogenic effect of ANP. Under this regimen, the hyponatremic effect of ANP was abolished. Then, we examined the site of Na + absorption in the alimentary tract by measuring the changes in ion composition of intraluminal fluid along the tract. Since Na + was absorbed at the esophagus and anterior/middle intestine, a sac was prepared at each site and the effects of ANP were examined in situ in conscious SW eels. ANP infusion did not alter Na + absorption at the esophagus, but it profoundly reduced the absorption at the intestine.Together with our previous finding that ANP does not alter renal Na + excretion, we propose that ANP reduces plasma Na + concentration in SW eels by inhibiting drinking and subsequent absorption of Na + by the intestine.

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Section: Discussionmentioning

confidence: 99%

Relative Potency of Three Homologous Natriuretic Peptides (ANP, CNP and VNP) in Eel Osmoregulation

Tsukada

Takei

2001

Zoological Science

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“…A partial sequence for a putative NPR-C/D also has been reported for the spiny dogfish (157). Radioligand binding protocols have localized NPR-A/B and NPR-C /D receptors in the gills of gulf toadfish (156), Atlantic hagfish (753), pouched lamprey (754), and New Zealand hagfish (72). It is generally assumed that the CNP/NPR-B couple is the ancestral vertebrate condition (297).…”

Section: Natriuretic Peptidesmentioning

confidence: 98%

The Multifunctional Fish Gill: Dominant Site of Gas Exchange, Osmoregulation, Acid-Base Regulation, and Excretion of Nitrogenous Waste

Evans¹,

Piermarini²,

Choe³

2005

Physiological Reviews

2,291

1,788

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The fish gill is a multipurpose organ that, in addition to providing for aquatic gas exchange, plays dominant roles in osmotic and ionic regulation, acid-base regulation, and excretion of nitrogenous wastes. Thus, despite the fact that all fish groups have functional kidneys, the gill epithelium is the site of many processes that are mediated by renal epithelia in terrestrial vertebrates. Indeed, many of the pathways that mediate these processes in mammalian renal epithelial are expressed in the gill, and many of the extrinsic and intrinsic modulators of these processes are also found in fish endocrine tissues and the gill itself. The basic patterns of gill physiology were outlined over a half century ago, but modern immunological and molecular techniques are bringing new insights into this complicated system. Nevertheless, substantial questions about the evolution of these mechanisms and control remain.

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“…The sections were stored in sealed slide boxes at -20°C until required. The sections were processed for autoradiography using rat (3-[ 125 I]iodotyrosol 28 ) atrial natriuretic peptide (termed 125 I-rANP) as a ligand, following the method of Donald et al (1994). Briefly, sections were preincubated at room temperature (20-22°C) for 15 min in 50 mM TRIS-(hydroxymethyl)aminomethane (TRIS)-HCl buffer (pH 7.4), 50 mM NaCl, 5 mM MgCl 2 , 0.1% bovine serum albumin and 0.05% bacitracin.…”

Section: Autoradiographymentioning

confidence: 99%

Atrial natriuretic peptide binding sites in the brain and pituitary gland of the toad, Bufo marinus : localisation and receptor characterisation

McLeod

Donald

2000

Cell and Tissue Research

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The distribution and nature of 125I-atrial natriuretic peptide binding sites have been examined in the brain and pituitary gland of the toad, Bufo marinus, using tissue section autoradiography, affinity cross-linking and electrophoresis, guanylyl cyclase assays and molecular analysis of natriuretic peptide receptor C (NPR-C) and NPR-GC mRNA expression. The highest density of 125I-atrial natriuretic peptide binding sites occurred in the dorsal pallium, the habenular region, the torus semicircularis, the choroid plexus, and the pituitary gland. Less dense binding was observed in the medial pallium, the thalamic region, the hypothalamus, the optic tectum, and the interpeduncular nucleus. The natriuretic peptide receptor-C specific ligand, C-ANF, displaced the binding in all brain regions; however, some residual binding was observed in the habenular region, the hypothalamus, the choroid plexus, and the pituitary gland. In isolated brain membranes, 1 microM rat atrial natriuretic peptide increased cyclic guanosine monophosphate levels to 90% above basal. Affinity cross-linking followed by reducing electrophoresis showed that 125I-atrial natriuretic peptide bound to proteins of 65 kDa and 135 kDa respectively. Furthermore, molecular analysis demonstrated that natriuretic peptide receptor-C and guanylyl cyclase messenger ribonucleic acid are expressed in the brain. In combination with the autoradiography, the data indicated that atrial natriuretic peptide acting via specific receptors could be important in natriuretic peptide regulation of the brain.

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Localization and analysis of natriuretic peptide receptors in the gills of the toadfish, Opsanus beta (teleostei)

Cited by 14 publications

References 22 publications

Relative Potency of Three Homologous Natriuretic Peptides (ANP, CNP and VNP) in Eel Osmoregulation

Relative Potency of Three Homologous Natriuretic Peptides (ANP, CNP and VNP) in Eel Osmoregulation

The Multifunctional Fish Gill: Dominant Site of Gas Exchange, Osmoregulation, Acid-Base Regulation, and Excretion of Nitrogenous Waste

Atrial natriuretic peptide binding sites in the brain and pituitary gland of the toad, Bufo marinus : localisation and receptor characterisation

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