Abstract:We demonstrated tomographic phase microscopy (TPM) of living diatoms in liquid in order to quantitatively analyze localization of cell components of symmetric shape cells. One TPM image includes several millions of pixel data of three-dimensional optical information such as refractive index (RI) values of cell components. In this work, the obtained TPM data of living cells were analyzed using X–Y cross sections to visualize the localization of cell components. Distribution of RI values at the cell surfaces and… Show more
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