Localised expression of OsIAA29 suggests a key role for auxin in regulating development of the dorsal aleurone of early rice grains

Basunia, Mafroz Ahmed; Nonhebel, Heather M.; Backhouse, David; McMillan, Mary

doi:10.1007/s00425-021-03688-z

Cited by 15 publications

(13 citation statements)

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“…Aux/IAA is the initial response gene of growth hormone, which is the hub of the growth hormone signaling process [ 33 ]. When growth hormone is at low concentration, Aux/IAA is synthesized in large quantities and the activity of growth hormone response factor (AUXIN RESPONSE FACTOR, ARF) is inhibited; when growth hormone is at higher concentration, the expression of Aux/IAA is degraded by ubiquitination and ARF is activated, and the activated ARF regulates downstream genes controlling the growth hormone signaling pathway [ 34 ]. The results of this study showed that the expression of Aux/IAA was up-regulated after enzymatic degradation compared with fresh young leaves, which indicated that the enzymatic digestion of the cells of young sugarcane leaves significantly affected the expression of Aux/IAA, which might have a certain effect on the response of protoplasts to regulate the growth hormone signaling process during heterozygous cell culture.…”

Section: Discussionmentioning

confidence: 99%

Transcriptome Analysis of Sugarcane Young Leaves and Protoplasts after Enzymatic Digestion

Zhang

Wang

Han

et al. 2022

Life

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Sugarcane somatic cell hybridization can break through the barrier of genetic incompatibility between distantly related species in traditional breeding. However, the molecular mechanisms of sugarcane protoplast regeneration and the conditions for protoplast preparation remain largely unknown. In this study, young sugarcane (ROC22) leaves were enzymatically digested, and the viability of protoplasts reached more than 90% after enzymatic digestion (Enzymatic combination: 2% cellulase + 0.5% pectinase + 0.1% dissociative enzyme + 0.3% hemicellulase, pH = 5.8). Transcriptome sequencing was performed on young sugarcane leaves and protoplasts after enzymatic digestion to analyze the differences in gene expression in somatic cells before and after enzymatic digestion. A total of 117,411 unigenes and 43,460 differentially expressed genes were obtained, of which 21,123 were up-regulated and 22,337 down-regulated. The GO terms for the 43,460 differentially expressed genes (DEGs) were classified into three main categories: biological process, cellular component and molecular function, which related to developmental process, growth, cell proliferation, transcription regulator activity, signal transducer activity, antioxidant activity, oxidative stress, kinase activity, cell cycle, cell differentiation, plant hormone signal transduction, and so on. After enzymatic digestion of young sugarcane leaves, the expressions of GAUT, CESA, PSK, CyclinB, CyclinA, CyclinD3 and cdc2 genes associated with plant regeneration were significantly down-regulated to 65%, 47%, 2%, 18.60%, 21.32%, 52% and 45% of young leaves, respectively. After enzymatic digestion, Aux/IAA expression was up-regulated compared with young leaves, and Aux/IAA expression was 3.53 times higher than that of young leaves. Compared with young leaves, these key genes were significantly changed after enzymatic digestion. These results indicate that the process of somatic enzymatic digestion process may affect the regeneration of heterozygous cells to a certain extent.

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Section: Discussionmentioning

confidence: 99%

Transcriptome Analysis of Sugarcane Young Leaves and Protoplasts after Enzymatic Digestion

Zhang

Wang

Han

et al. 2022

Life

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“…An aleurone-specific NF-Y family member, OsNF-YB1, binds to promotors of genes encoding OsSUT1, OsSUT3 and OsSUT4, as well as the auxin biosynthetic gene, OsYUC11. The latter gene product contributes to the transcriptional network controlling auxin levels regulating endosperm differentiation (Bai et al, 2016;Basunia et al, 2021;Xu et al, 2021) and enhancing SUT activities (Fig. 6d; Darussalam et al, 1998;Basunia & Nonhebel, 2019).…”

Section: Regulation Of Sucrose Efflux From Maternal Tissuesmentioning

confidence: 99%

Sugar loading of crop seeds – a partnership of phloem, plasmodesmal and membrane transport

2023

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Summary Sugar loading of developing seeds comprises a cohort of transport events that contribute to reproductive success and seed yield. Understanding these events is most advanced for grain crops (Brassicaceae, Fabaceae and Gramineae) and Arabidopsis. For these species, 75–80% of their final seed biomass is derived from phloem‐imported sucrose. Sugar loading consecutively traverses three genomically distinct, and symplasmically isolated, seed domains: maternal pericarp/seed coat, filial endosperm and filial embryo. Sink status of each domain co‐ordinately transitions from growth to storage. The latter is dominated by embryos (Brassicaceae and Fabaceae) or endosperms (Gramineae). Intradomain sugar transport occurs symplasmically through plasmodesmata. Interdomain sugar transport relies on plasma‐membrane transporters operating in efflux (maternal and endosperm) or influx (endosperm and embryo) modes. Discussed is substantial progress made in identifying, and functionally evaluating, sugar symporters (STPs, SUTs or SUCs) and uniporters (SWEETs). These findings have underpinned a mechanistic understanding of seed loading. Less well researched are possible physical limitations imposed by hydraulic conductivities of differentiating protophloem and of subsequent plasmodesmal transport. The latter is coupled with sugar homeostasis within each domain mediated by sugar transporters. A similar conclusion is ascribed to fragmentary understanding of regulatory mechanisms integrating transport events with seed growth and storage.

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“…Additionally, maize is widely used in agriculture, animal husbandry and industry, for items such as food, animal feed and biofuel production ( Shiferaw et al, 2011 ). Therefore, maize has great economic value, and research on the molecular mechanism of maize growth and development will help producers better guide its daily production ( Basunia et al, 2021 ).…”

Section: Introductionmentioning

confidence: 99%

ZmIAA5 regulates maize root growth and development by interacting with ZmARF5 under the specific binding of ZmTCP15/16/17

Yang

Shi

Zhao

et al. 2022

PeerJ

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Background The auxin indole-3-acetic acid (IAA) is a type of endogenous plant hormone with a low concentration in plants, but it plays an important role in their growth and development. The AUX/IAA gene family was found to be an early sensitive auxin gene with a complicated way of regulating growth and development in plants. The regulation of root growth and development by AUX/IAA family genes has been reported in Arabidopsis, rice and maize. Results In this study, subcellular localization indicated that ZmIAA1-ZmIAA6 primarily played a role in the nucleus. A thermogram analysis showed that AUX/IAA genes were highly expressed in the roots, which was also confirmed by the maize tissue expression patterns. In maize overexpressing ZmIAA5, the length of the main root, the number of lateral roots, and the stalk height at the seedling stage were significantly increased compared with those of the wild type, while the EMS mutant zmiaa5 was significantly reduced. The total number of roots and the dry weight of maize overexpressing ZmIAA5 at the mature stage were also significantly increased compared with those of the wild type, while those of the mutant zmiaa5 was significantly reduced. Yeast one-hybrid experiments showed that ZmTCP15/16/17 could specifically bind to the ZmIAA5 promoter region. Bimolecular fluorescence complementation and yeast two-hybridization indicated an interaction between ZmIAA5 and ZmARF5. Conclusions Taken together, the results of this study indicate that ZmIAA5 regulates maize root growth and development by interacting with ZmARF5 under the specific binding of ZmTCP15/16/17.

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Localised expression of OsIAA29 suggests a key role for auxin in regulating development of the dorsal aleurone of early rice grains

Abstract: Main ConclusionNon-canonical AUX/IAA protein, OsIAA29, and ZmMPR-1 homologues, OsMRPLs, are part of an auxin-related signalling cascade operating in the dorsal aleurone during early rice grain development.

Cited by 15 publications

References 53 publications

Transcriptome Analysis of Sugarcane Young Leaves and Protoplasts after Enzymatic Digestion

Transcriptome Analysis of Sugarcane Young Leaves and Protoplasts after Enzymatic Digestion

Sugar loading of crop seeds – a partnership of phloem, plasmodesmal and membrane transport

ZmIAA5 regulates maize root growth and development by interacting with ZmARF5 under the specific binding of ZmTCP15/16/17

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