Localisation of nucleolus-organising regions in interphase cells

Goessens, Guy

doi:10.1007/bf00236896

Cited by 25 publications

(6 citation statements)

References 8 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In addition, these observations have dem onstrated that the location of the silverstained spherules and the site of rDNA (as revealed by hybridization in situ) coincide with the site of the nucleolar fibrillar center. The data are in agreement with the role assigned to the fibrillar center, which has been identified as the site of the NOR in interphase nuclei (H ernandez-V erdun Goessens, et al, 1979). We have stained fibrillar centers as described by Hi rnandez-V erdun et al (1980) and Schwarzacher et al (1978) using a modification of the NOR-silver tech nique adapted for electron microscopy.…”

Section: Discussionsupporting

confidence: 79%

See 1 more Smart Citation

Sex vesicle-associated nucleolar organizers in mouse spermatocytes: localization, structure, and function

Knibiehler¹,

Mirre²,

Hartung³

et al. 1981

Cytogenet Genome Res

View full text Add to dashboard Cite

Selective silver staining demonstrated that autosomal bivalents containing transcriptively active nucleolar organizers associated with the sex vesicle during pachytene of mouse spermatocytes. Later in pachytene, the nucleolar organizers covered the portion of the sex vesicle furthest from the attachment to the nuclear envelope. Hybridization in situ revealed the presence of rDNA in the silver-positive material. The nucleolus, formed from an autosomal bivalent, exhibited a large fibrillar center surrounded by an electron-opaque fibrillar zone. The nucleolar association with the sex vesicle was studied at early, middle, and late pachytene by hybridization in situ, NOR silver staining, and electron microscopy. These observations enabled us to further define the relationships of the nucleolar components with the X-Y pair.

show abstract

Section: Discussionsupporting

confidence: 79%

“…5). The components described above were also found in the fused nucle olus; i.e., a fibrillar center was formed as a result of the fusion of two fibrillar centers belonging to each small nucleolus and the associated electron-opaque fibrillar layer, which is known to be the site of rDNA transcription Stahl, 1978a, h, 1981;Goessens, 1979).…”

Section: Mid Pachytenementioning

confidence: 99%

Sex vesicle-associated nucleolar organizers in mouse spermatocytes: localization, structure, and function

Knibiehler¹,

Mirre²,

Hartung³

et al. 1981

Cytogenet Genome Res

View full text Add to dashboard Cite

show abstract

“…The secondary constrictions share common properties with the FCs. They are totally fibrous in ultrastructure (BRINKLEY 1965;RECHER et al 1972;GoESSENS and LEPOINT 1979) and have the capacity for selective staining with silver (HERNANDEZ-VERDUN et al 1978;SCHWARZACHER et al 1978;GoESSENS 1979). With gradual condensation of chromosomes accompanying concurrent NOR inactivation, the number of FCs decreases and at metaphase it corresponds to the number of silver-stained NORs (RoZAK et al 1986).…”

Section: Discussionmentioning

confidence: 99%

Structural differentiation of the nucleolus-organizing regions between mitotic and epidermal cells inVicia faba

Sato

1992

Caryologia

View full text Add to dashboard Cite

SUMMARY-The nucleolar secondary constrictions of Vicia faba chromosomes were filled with a weakly stained substance when the sections were treated with conventional double staining and examined under an electron microscope. But both silver staining and ribonucleoprotein preferential staining contrasted these constrictions as positively stained areas. On the other hand, the nucleolus-associated chromatin blocks in the epidermal cells were clearly impregnated with silver although they were fully condensed, suggesting that ribosomal (r) RNA genes are organized into the condensed chromosomal segment when cells differentiate into specialized cells such as the epidermal cells.

show abstract

“…Some results have shown that fibrillar centers correspond to the nucleolusorganizer regions (NORs) containing the genes coding for ribosomal RNA (rRNA) [4,[10][11][12]. NORs can be selectively detected by silver staining methods [3,13].…”

Section: Introductionmentioning

confidence: 99%

“…In different types of nucleoli of somatic cells [3,4], cultivated cells [5,6] or sexual cells [7] a structure named the fibrillar center has been observed. It was first described by Recher et al [8) and reviewed by Goessens and Lepoint [9].…”

Section: Introductionmentioning

confidence: 99%

Silver staining analysis on spermatozoa ofNucella lapillus(Gastropoda, Prosobranchia)

Azevedo¹,

Castilho²,

Barandela³

1986

International Journal of Invertebrate Reproduction and Developm

View full text Add to dashboard Cite

Sperm of Nucel/a lapillus was studied by electron microscopy, including the application of a cytochemical silver method. Using silver impregnation a dense precipitation of Ag granules in spermatocyte II nucleoli was seen over the fibrillar component and a slight one in the granular component. On longitudinal sections of the spermatozoon the results demonstrate that argyrophilic proteins are located in the external limiting zone of the acrosome in the anterior portion of the nucleus between the cytoplasmic and the nuclear membranes, in the posterior end of the nucleus and in the terminal portion of the middle region. These data indicate an affinity for silver in areas of the cytoplasm containing microtubules and in zones of transition. silver staining; acid proteins; spermatozoa; Gastropoda

show abstract

Localisation of nucleolus-organising regions in interphase cells

Abstract: A technique based on the use of silver solutions, which selectively stains the nucleolus-organising regions (NORs) in chromosomes, was applied to interphase Ehrlich tumour cells. The results indicate that nucleolar fibrillar centres correspond to the NORs.

Cited by 25 publications

References 8 publications

Sex vesicle-associated nucleolar organizers in mouse spermatocytes: localization, structure, and function

Sex vesicle-associated nucleolar organizers in mouse spermatocytes: localization, structure, and function

Structural differentiation of the nucleolus-organizing regions between mitotic and epidermal cells inVicia faba

Silver staining analysis on spermatozoa ofNucella lapillus(Gastropoda, Prosobranchia)

Contact Info

Product

Resources

About