Localisation of neurokinin 3 (NK3) receptor immunoreactivity in the rat gastrointestinal tract

Mann, Patricia T.; Southwell, Bridget R.; Ding, Yu; Shigemoto, Ryuichi; Mizuno, Noboru; Furness, John B.

doi:10.1007/s004410050846

Cited by 82 publications

(70 citation statements)

References 32 publications

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“…The NK3 receptor is primarily expressed in the central nervous system but its presence was also detected in specific peripheral tissues, such as human ovary, uterus (Roman et al 2012), kidney, lung ), rat and mouse oesophagus (Mann et al 1997;Wang et al 2002). The present results seem to confirm the above findings because TACR3 mRNA was strongly expressed in the hippocampus, hypothalamus, pituitary gland and spinal cord while its expression in peripheral tissues was very low or undetectable.…”

Section: Oryctolagus Cuniculus Nk2r Sus Scrofa Nk2rsupporting

confidence: 84%

“…We failed to observe any NK3R mRNA signal in the gastrointestinal tract, or detected it only faintly. Other reports have revealed its presence, but only in nerve cell bodies of the plexus (according to the strict definition of the latter; Mann et al 1997). Together with NK1R and NK2R, NK3R regulates intestinal contractility (Valero et al 2011).…”

Section: Oryctolagus Cuniculus Nk2r Sus Scrofa Nk2rmentioning

confidence: 99%

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Characterisation, localisation and expression of porcine TACR1, TACR2 and TACR3 genes

Jakimiuk¹,

Podlasz²,

Chmielewska-Krzesińska³

et al. 2017

Vet. Med.

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Substance P is involved in many physiological and pathophysiological processes. This functional diversity is mediated by three neurokinin receptor subtypes (NK1R, NK2R and NK3R) encoded by the TACR1, TACR2 and TACR3 genes, respectively. Despite the increasing interest in using pigs (Sus scrofa) to study human disease mechanisms, the sequences of these receptors are still unconfirmed or in the case of the NK1 receptor, not yet even unpredicted. We employed in silico analysis to define the localisation of the porcine tachykinin receptor genes, and to predict the structures and amino acid sequences of the respective proteins. A reverse transcription polymerase chain reaction (RT-PCR) assay was performed to analyse the expression of tachykinin receptor genes in different porcine tissues. The data show that the TACR1 gene is located on chromosome 3, TACR2 on chromosome 14 and TACR3 on chromosome 8. All three genes encode proteins with structures that incorporate features of G-protein-coupled receptors with sizes of 407, 381 and 464 amino acids, respectively. The receptors display a high degree of similarity to other mammalian neurokinin receptors. The NK1R subtype is expressed in both the central nervous system and peripheral tissues, while NK2R expression seems to be localised mostly to peripheral tissues. The expression of NK3R is found mainly in the central nervous system. This report provides for the first time the results of a comprehensive analysis of the structure and distribution of porcine NK1R, as well as other porcine neurokinin receptors and their genes. We hope that our data may offer an invaluable foundation for the future studies on the function of diverse tachykinin peptides in the central nervous system and peripheral tissues.

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Section: Oryctolagus Cuniculus Nk2r Sus Scrofa Nk2rsupporting

confidence: 84%

Section: Oryctolagus Cuniculus Nk2r Sus Scrofa Nk2rmentioning

confidence: 99%

Characterisation, localisation and expression of porcine TACR1, TACR2 and TACR3 genes

Jakimiuk¹,

Podlasz²,

Chmielewska-Krzesińska³

et al. 2017

Vet. Med.

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“…This particular difference could be a result of tissue sampling and distribution of NF-M-and Cal-immunoreactivity. Mann et al (1997Mann et al ( , 1999a observed that most Cal-IRN also expressed Calr-, NK-1R-, and NK-3R-immunoreactivity. The results of the present study are in partial agreement with the two previous studies by Mann et al We found that Cal-IRN exhibits rather low levels of colocalization with NK-1R-IRN (7.31-27.05%) and Calr-IRN (2.85-34.42%) in myenteric and submucosal plexus, respectively.…”

Section: Discussionmentioning

confidence: 99%

Morphology and distribution of nitric oxide synthase‐, neurokinin‐1 receptor‐, calretinin‐, calbindin‐, and neurofilament‐M‐immunoreactive neurons in the myenteric and submucosal plexuses of the rat small intestine

Sayegh

Ritter

2003

Anat. Rec.

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Characterization of the enteric neurons is vital for understanding their physiological role. We have used single and dual label fluorescence and peroxidase-based immunohistochemistry in myenteric and submucosal whole mounts from the rat small intestine to evaluate the morphology and distribution of enteric neurons immunoreactive for the following phenotypic antigens: neuronal nitric oxide synthase (NOS), neurokinin-1 receptor (NK-1R), calretinin (Calr), calbindin (Cal), and neurofilament-M (NF-M). NOS-immunoreactive neurons had Dogiel type I morphology, were abundant in the myenteric plexus compared to the submucosal plexus, and never coexpressed NK-1R immunoreactivity. NK-1R-and Calr-immunoreactive neurons had Dogiel type II morphology and were distributed comparably in both plexuses. NK-1R and Calr-immunoreactivity were coexpressed in many of the same neurons. Calbindin-immunoreactive neurons exhibited four distinct morphologies: small and large Dogiel type II neurons, Dogiel type I neurons, and small elongated neurons. These neurons were significantly fewer in number in the myenteric plexus compared to the submucosal plexus. Neurofilament-M-immunoreactive neurons had three morphologies, Dogiel type II neurons, small Dogiel type II neurons, and a less common subpopulation of small, elongated, multipolar neurons. These neurons were also fewer in number in the myenteric plexus compared to the submucosal plexus. The distribution of these phenotypic markers may assist future work that elucidates the functional activities of these enteric neurons such as control of intestinal motility and adaptation to the entry of gastric contents. Anat Rec Part A 271A: 209 -216, 2003.

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“…NK1R and NK3R are coexpressed by enteric neurons (15,16) and endothelial cells (17). SP and NKA, the principal tachykinins in peripheral tissues, can interact with both NK1R and NK3R (14).…”

Section: Discussionmentioning

confidence: 99%

“…Some cells, such as enteric neurons (15,16) and microvascular endothelial cells (17), coexpress NK1R and NK3R. However, the consequences of selectively activating one NKR on signaling and trafficking of another are unknown.…”

mentioning

confidence: 99%

Heterologous regulation of trafficking and signaling of G protein-coupled receptors: β-Arrestin-dependent interactions between neurokinin receptors

Schmidlin

Déry

Bunnett

et al. 2002

Proc. Natl. Acad. Sci. U.S.A.

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Cells express multiple G protein-coupled receptors that are simultaneously or sequentially activated by agonists. The consequences of activating one receptor on signaling and trafficking of another receptor are unknown. We examined the effects of selective activation of the neurokinin 1 receptor (NK1R) on signaling and trafficking of the NK3R and vice versa. Selective agonists of NK1R and NK3R induced membrane translocation of ␤-arrestins (␤-ARRs). Dominant negative ␤-ARR 319 -418 inhibited endocytosis of NK1R and NK3R. Whereas an NK1R agonist caused sequestration of NK1R with ␤-ARR in the same endosomes, thereby depleting them from the cytosol, ␤-ARRs did not prominently sequester with the activated NK3R and rapidly returned to the cytosol. In cells coexpressing both receptors, prior activation of the NK1R inhibited endocytosis and homologous desensitization of the NK3R, which was dose-dependently reversed by overexpression of ␤-ARR1. Similar results were obtained in enteric neurons that naturally coexpress the NK1R and NK3R. In contrast, activation of the NK3R did not affect NK1R endocytosis or desensitization. Thus, the high-affinity and prolonged interaction of the NK1R with ␤-ARRs depletes ␤-ARRs from the cytosol and limits their role in desensitization and endocytosis of the NK3R. Because ␤-ARRs are critical for desensitization, endocytosis, and mitogenic signaling of many receptors, this sequestration is likely to have important and widespread implications.

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Localisation of neurokinin 3 (NK3) receptor immunoreactivity in the rat gastrointestinal tract

Cited by 82 publications

References 32 publications

Characterisation, localisation and expression of porcine TACR1, TACR2 and TACR3 genes

Characterisation, localisation and expression of porcine TACR1, TACR2 and TACR3 genes

Morphology and distribution of nitric oxide synthase‐, neurokinin‐1 receptor‐, calretinin‐, calbindin‐, and neurofilament‐M‐immunoreactive neurons in the myenteric and submucosal plexuses of the rat small intestine

Heterologous regulation of trafficking and signaling of G protein-coupled receptors: β-Arrestin-dependent interactions between neurokinin receptors

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