Localisation by autoradiography of neuronal 5-HT3 receptors in the mouse CNS

Waeber, Christian; Dixon, Kendal C.; Hoyer, Daniel; Palacios, J.M.

doi:10.1016/0014-2999(88)90825-4

Cited by 162 publications

(48 citation statements)

References 5 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Serotonin receptors are found in high concentrations in the area postrema of the brain stem. [21][22][23] Unfortunately, the plasma half-life of serotonin is only a few minutes. As a result, the hypothesis proposed by Goll et al 4 that intestinal ischemia releases intravascular serotonin to induce PONV has never been investigated directly with plasma serotonin measurements.…”

Section: Discussionmentioning

confidence: 99%

Supplemental oxygen does not prevent postoperative nausea and vomiting after gynecological laparoscopy

McKeen

Arellano

O’Connell

2009

Can J Anesth/J Can Anesth

View full text Add to dashboard Cite

Purpose Postoperative nausea and vomiting are among the most common and distressing side effects of general anesthesia. Supplemental intraoperative 80% oxygen reduces postoperative nausea and vomiting following open and laparoscopic abdominal surgery. However, this benefit has not been observed in other patient populations. We undertook this study to evaluate the effect of 80% supplemental intraoperative oxygen on the incidence of postoperative nausea and vomiting following ambulatory surgery for laparoscopic tubal ligation. Methods Following Research Ethics Board approval, 304 subjects were enrolled into one of two arms of a randomized prospective controlled study. The intervention group (n = 147) breathed 80% oxygen and the control group (n = 145) breathed routine 30% oxygen (balance medical air) while both groups were receiving a standardized general anesthetic. Nausea was assessed as: none, mild, moderate, or severe; vomiting was any emetic episode or retching. Any assessment either greater than none (nausea) or greater than zero (vomiting) was considered positive. Results The incidence of postoperative nausea and vomiting up to 24 hr following surgery was 69% in the 80% oxygen intervention group and 65% in the 30% oxygen control group (P = 0.62). There were no differences in nausea alone, vomiting, or antiemetic use in the postoperative anesthetic care unit or at any time (pre-or postdischarge) up to 24 hr after surgery. Conclusions This trial of 304 women did not demonstrate that administering intraoperative supplemental 80% oxygen during ambulatory surgery for laparoscopic tubal ligation prevented postoperative nausea or vomiting during the initial postoperative 24 hr compared with women who received routine 30% oxygen.

show abstract

Section: Discussionmentioning

confidence: 99%

Supplemental oxygen does not prevent postoperative nausea and vomiting after gynecological laparoscopy

McKeen

Arellano

O’Connell

2009

Can J Anesth/J Can Anesth

View full text Add to dashboard Cite

show abstract

“…2 IT opioid-induced itching may be related to the cephalad spread of the drug in the cerebrospinal fluid and its action on both µ receptors (in the medulla), 5,9,10 and central serotonin type 3 receptors (in the spinal cord and medulla). 11,12 Therefore, opioids that do not cause peripheral histamine release can still cause itching. 2,3 Opioids administered (both systemically and regionally) reach central neural centres.…”

Section: R Re Es Su Ul Lt Ts Smentioning

confidence: 99%

L’administration prophylactique d’ondansétron ne réduit pas l’incidence de prurit induit par le sufentanil intrathécal

Waxler

Mondragon

Patel

et al. 2004

Can J Anesth/J Can Anesth

View full text Add to dashboard Cite

P Pu ur rp po os se e: : Postoperative itching after intrathecal (IT) narcotics may be a difficult and important problem for both the anesthesiologist and the patient in the postanesthetic care unit. Since some studies have reported success in preventing itching with ondansetron, we designed a prospective, randomized, double-blinded, and controlled study to test whether prophylactic iv ondansetron effectively reduces the incidence of IT sufentanil-induced pruritus.M Me et th ho od ds s: : Thirty-four patients (ASA I-III, age 18-74 yr) underwent ambulatory surgery after spinal anesthesia with IT lidocaine (15-100 mg) and IT sufentanil (10 µg). The patients were randomized into two groups to receive iv either 4 mL saline (n = 13) or 8 mg ondansetron (n = 21) before the IT injection. The incidence of pruritus and other variables was recorded. Pruritus scores were obtained with a verbal analogue score with 0 meaning none and 10 the worst itching that the patient could imagine. Statistical difference was assumed if P < 0.05. R Re es su ul lt ts s: : Ondansetron did not reduce the incidence of pruritus (77 vs 81%) compared to placebo (P = 1.000). The pruritus scores (4.4 vs 3.6) of the two groups were not significantly different (P = 0.670).C Co on nc cl lu us si io on ns s: : There are contradictory findings in the literature regarding the effectiveness of ondansetron in preventing narcoticinduced itching. Although some studies have indicated that ondansetron could prevent this side effect of IT narcotics, a recent report suggested that ondansetron is not effective in preventing narcotic-induced itching (sufentanil-morphine) after a Cesarean section. In the present study we obtained similar, negative results. Objectif : Le prurit postopératoire induit par l'administration intrathé-cale (IT) de narcotiques est un problème important pour l'anesthésio-logiste et le patient en salle de réveil. Certaines études ont montré que l'ondansétron prévenait le prurit. Notre étude prospective, randomisée, contrôlée et à double insu voulait vérifier si l'administration iv préventive d'ondansétron réduit effectivement l'incidence de prurit induit par le sufentanil IT. Méthode : Trente-quatre patients (ASA I-III, de 18 à 74 ans) ont subi une intervention chirurgicale ambulatoire sous rachianesthésie avec de la lidocaïne IT (15-100 mg) et du sufentanil IT (10 µg). Les patients, randomisée en deux groupes, ont reçu soit 4 mL de solution saline iv (n = 13), soit 8 mg d'ondansétron (n = 21) avant l'injection IT. L'incidence de prurit et d'autres variables a été notée. Les scores de

show abstract

“…Recently, 5-HT3 receptors have been observed in the brain and have been implicated in many central nervous system processes (11, 12). High levels of 5-HT3 radioligand binding have been detected in the area postrema and solitary tract nucleus (13,14). The antiemetic effects of 5-HT3 antagonists may be attributed to actions at these sites (15,16).…”

Section: Introductionmentioning

confidence: 99%

Nervous system distribution of the serotonin 5-HT3 receptor mRNA.

Tecott

Maricq

Julius

1993

Proc. Natl. Acad. Sci. U.S.A.

321

177

View full text Add to dashboard Cite

The serotonin 5-HT3 receptor subtype has been implicated in many brain functions. Antagonists of this receptor have anxiolytic and antiemetic effects in humans and in animal models. To determine with cellular resolution the distribution of 5-HT3 receptor mRNA, in situ hybridization was performed in sections of mouse brain and dorsal root ganglia. Scattered labeled cells were observed throughout cortical regions, with highest densities in the piriform, cingulate, and entorhinal areas. Strong hybridization signals were seen in the hippocampal formation, where expression appeared primarily in interneurons. Labeled cells were most abundant in the posteroventral hippocampal region, particularly in the lacunosum moleculare layer of CAL. This distribution suggests that 5-HT3 receptors may mediate the known serotonergic inhibition of pyramidal cell populations via excitation of inhibitory interneurons. Labeled cells were also observed in the major subdivisions of the amygdaloid complex, the olfactory bulb, the trochlear nerve nucleus, the dorsal tegmental region, the facial nerve nucleus, the nucleus of the spinal tract of the trigeminal nerve, and the spinal cord dorsal horn. In the periphery, intense hybridization signals were seen in a subpopulation of cells in dorsal root ganglia. The data correlate generally with physiological, behavioral, and receptor autoradiographic studies, provide cellular resolution, and reveal regions of receptor expression not previously observed. The distribution of 5-HT3 receptor mRNA is consistent with roles for the receptor in cognition and affect and in the modulation of sensory input.The monoamine serotonin (5-hydroxytryptamine) has been implicated in a variety of central and peripheral nervous system processes. Its diverse effects are mediated by subclasses of serotonin receptors that were initially defined on the basis of distinct ligand binding profiles (1). Biochemical and molecular genetic characterization of these receptors demonstrates that the 5-HT1, 5-HT2, and 5-HT4 receptor subclasses belong to the superfamily of guanine nucleotide binding protein-coupled receptors (2, 3). In contrast, serotonin 5-HT3 receptors are ligand-gated ion channels that mediate fast excitatory responses (4-6).Responses mediated by 5-HT3 receptors have been characterized in the peripheral nervous system, suggesting roles for the receptor in nociception and gut motility (7)(8)(9)(10). Recently, 5-HT3 receptors have been observed in the brain and have been implicated in many central nervous system processes (11, 12). High levels of 5-HT3 radioligand binding have been detected in the area postrema and solitary tract nucleus (13,14). The antiemetic effects of 5-HT3 antagonists may be attributed to actions at these sites (15,16). Receptor autoradiographic studies have also demonstrated localization of 5-HT3 receptors to cortical and limbic system regions (14,17,18). 5-HT3 receptor antagonists have anxiolytic effects in humans and in animal models (12, 19), perhaps through action at these sites. Re...

show abstract

Localisation by autoradiography of neuronal 5-HT3 receptors in the mouse CNS

Cited by 162 publications

References 5 publications

Supplemental oxygen does not prevent postoperative nausea and vomiting after gynecological laparoscopy

Supplemental oxygen does not prevent postoperative nausea and vomiting after gynecological laparoscopy

L’administration prophylactique d’ondansétron ne réduit pas l’incidence de prurit induit par le sufentanil intrathécal

Nervous system distribution of the serotonin 5-HT3 receptor mRNA.

Contact Info

Product

Resources

About