Local Transplantation of G-CSF-Mobilized CD34<sup>+</sup>Cells in a Patient with Tibial Nonunion: A Case Report

Kuroda, Ryosuke; Matsumoto, Tomoyuki; Miwa, Masahiko; Kawamoto, Atsuhiko; Mifune, Yutaka; Fukui, Tomoaki; Kawakami, Yoshiyuki; Niikura, Takahiro; Lee, Sang Yang; Oe, Keisuke; Shoji, Taro; Kuroda, Tomoya; Horii, Miki; Yokoyama, Ayumi; Ono, Takayuki; Koibuchi, Yasushi; Kawamata, Shin; Fukushima, Masanori; Kurosaka, Masahiro; Asahara, Takayuki

doi:10.3727/096368910x550189

Cited by 39 publications

(32 citation statements)

References 29 publications

(34 reference statements)

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“…As a pilot case from a phase I/IIA clinical trial, Kuroda et al [41] reported the results of transplantation of autologous peripheral blood CD34? cells, the hematopoietic/EPCenriched population, in a patient with non-union of a tibia fracture.…”

Section: Future Perspectives and Clinical Rationalementioning

confidence: 98%

An emerging cell-based strategy in orthopaedics: endothelial progenitor cells

Ateşok

Matsumoto

Karlsson

et al. 2012

Knee Surg Sports Traumatol Arthrosc

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Section: Future Perspectives and Clinical Rationalementioning

confidence: 98%

An emerging cell-based strategy in orthopaedics: endothelial progenitor cells

Ateşok

Matsumoto

Karlsson

et al. 2012

Knee Surg Sports Traumatol Arthrosc

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“…More than 10 clinical trials [4][5][6][8][9][10][11][12][13][14] have been performed to elucidate the therapeutic effects of EPCs. These studies have provided information regarding the feasibility of using EPCsbased therapy to promote vasculogenesis.…”

Section: Discussionmentioning

confidence: 99%

“…[2][3][4][5][6] Kocher, et al 7) infused freshly isolated human EPCs into a nude rat model of myocardial ischemia and observed preservation of left ventricular (LV) function and inhibition of cardiac apoptosis. More than 10 clinical trials [4][5][6][8][9][10][11][12][13][14] have been performed to elucidate the therapeutic effects of EPCs. These studies have provided information regarding the feasibility of using EPCsbased therapy to promote vasculogenesis.…”

Section: N the Last Decade Several Types Of Cells Have Been Utilizedmentioning

confidence: 99%

Mechanical Stress Regulates Endothelial Progenitor Cell Angiogenesis Through VEGF Receptor Endocytosis

et al. 2016

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SummaryThe clinical goal of cell-based treatment for chronic heart failure is to coordinately reconstitute the cardiomyocytes and associated circulation environment including coronary resistance arteries, arterioles, and capillary profiles.1) This goal can be possibly achieved by implementing multipotent adult stem cells. However, it remains a challenge to modify the capillary network in the decompensated heart. A mechanical stress model was used in this study to mimic the hemodynamic and hormonal states of the decompensated heart in vitro. The angiogenesis role of endothelial progenitor cells (EPCs) under stress has been well-recognized in vascular repair. We investigated the molecular mechanisms of EPCs in this model. We found that expression of vascular endothelial growth factor (VEGF) in EPCs was significantly decreased by mechanical stress, and this effect was accompanied by a decrease in angiogenesis in vitro. Interestingly, the defective angiogenesis can be reversed by upregulating the membrane VEGF receptor (VEGFR) endocytosis. An atypical protein kinase C (aPKC) inhibitor can promote the VEGFR internalization in EPCs and enhance the formation of vascular networks. Thus, the upregulation of VEGFR endocytosis in EPCs could be a potential therapy for the cell-based treatment of chronic heart failure by enhancing the cardiomyocytes. (Int Heart J 2016; 57: 356-362) Key words: Cell-based treatment, Capillary network I n the last decade, several types of cells have been utilized in cell-based therapies to treat acute myocardial infarction and chronic heart failure (CHF). Endothelial progenitor cells (EPCs) are a useful cell type that has been tested experimentally in patients with encouraging results.2-6) Kocher, et al 7) infused freshly isolated human EPCs into a nude rat model of myocardial ischemia and observed preservation of left ventricular (LV) function and inhibition of cardiac apoptosis. More than 10 clinical trials [4][5][6][8][9][10][11][12][13][14] have been performed to elucidate the therapeutic effects of EPCs. These studies have provided information regarding the feasibility of using EPCsbased therapy to promote vasculogenesis. Despite these promising results, the search for a means to reverse the negative remodeling associated with CHF remains a concern. 15) This search is complicated by numerous factors that influence the growth and behavior of harvested cells. These factors include the age of the organ, the pathological state, and the severity of the cardiac disease. 16,17) Additionally, insufficient oxygen supply provided by the existing capillary network further complicates the extent of the therapeutic benefit provided by stem/progenitor cells. Editorial p.268Currently, the means of modifying the regeneration and integrity of the capillary network in the failing and hypertrophied decompensated heart are limited clinically, and the underlying mechanism remains unclear. The viability, proliferation, migration, and angiogenesis of EPCs are four critical determinants of revascularization in...

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“…Fixation methods included fixation with autologous bone grafting (n=78), fixation revision (n=8), autologous bone grafting without fixation revision (n=7), en bloc resection of the infected bone at the first stage and reconstruction using bone lengthening at the second stage (n=2), and transplantation of peripheral blood CD34-positive cells 14 and autologous bone grafting (n=7). Bone substitute or bone morphogenetic protein was not used.…”

Section: Methodsmentioning

confidence: 99%

Outcome of Fixation for Nonunion of Extremities

Niikura

Lee

Sakai

et al. 2014

J Orthop Surg (Hong Kong)

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Purpose. To review the outcomes of 102 consecutive patients who underwent fixation for nonunion of extremities. Methods. Medical records of 71 men and 31 women aged 16 to 82 (mean, 45; standard deviation [SD], 19) years who underwent fixation for nonunion of the upper (n=31) and lower (n=71) extremities were reviewed. The nonunion types included oligotrophic (n=47), hypertrophic (n=22), atrophic (n=17), defect (n=12), and comminuted (n=4). 13 of the nonunions were infected. 10 patients had concomitant injuries of the brain (n=2), abdomen (n=5), chest (n=1), and spine (n=2). 43 patients were smokers, and 19 had diabetes mellitus. 80 patients had had previous surgery, and 3 had undergone previous radiotherapy. The mean time from injury to nonunion surgery was 19 (SD, 25.6; range, months. Outcome measures included bone union, ability to return to original work, pain, gait, and loss of range of motion. Results. The mean follow-up period was 26.6 (SD,

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Local Transplantation of G-CSF-Mobilized CD34⁺Cells in a Patient with Tibial Nonunion: A Case Report

Cited by 39 publications

References 29 publications

An emerging cell-based strategy in orthopaedics: endothelial progenitor cells

An emerging cell-based strategy in orthopaedics: endothelial progenitor cells

Mechanical Stress Regulates Endothelial Progenitor Cell Angiogenesis Through VEGF Receptor Endocytosis

Outcome of Fixation for Nonunion of Extremities

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Local Transplantation of G-CSF-Mobilized CD34+Cells in a Patient with Tibial Nonunion: A Case Report

Cited by 39 publications

References 29 publications

An emerging cell-based strategy in orthopaedics: endothelial progenitor cells

An emerging cell-based strategy in orthopaedics: endothelial progenitor cells

Mechanical Stress Regulates Endothelial Progenitor Cell Angiogenesis Through VEGF Receptor Endocytosis

Outcome of Fixation for Nonunion of Extremities

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Local Transplantation of G-CSF-Mobilized CD34⁺Cells in a Patient with Tibial Nonunion: A Case Report