Local stimulation of cultured myocyte cells by femtosecond laser-induced stress wave

Kuo, Yung En; Wu, Cheng Chi; Hosokawa, Yoichiroh; Maezawa, Yasuyo; Okano, Kazunori; Masuhara, Hiroshi; Kao, Fu Jen

doi:10.1007/s00339-010-5927-2

Cited by 11 publications

(8 citation statements)

References 3 publications

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“…Besides, it has been applied to fabricate adhesive substrates to pattern cells under a culturing condition, and to control cell migration and cell-cell interactions [ [14] , [15] , [16] ]. Furthermore, NIR fs laser pulses have been used to stimulate cells of varied types (HeLa, PC12, P19CL6 and C2C12) and tissue regeneration was studied [ 17 ]. Meanwhile, it has been shown that NIR fs laser pulses (single or multiple pulses) of moderate energy (0.1–10 nJ/pulse) can generate transient holes on the plasma membrane of cells, which then allows introducing external macromolecules into living cells [ [18] , [19] , [20] , [21] ].…”

Section: Introductionmentioning

confidence: 99%

Selective induction of targeted cell death and elimination by near-infrared femtosecond laser ablation

Okano

Wang

Hong

et al. 2020

Biochemistry and Biophysics Reports

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The techniques for inducing the death of specific cells in tissue has attracted attention as new methodologies for studying cell function and tissue regeneration. In this study, we show that a sequential process of targeted cell death and removal can be triggered by short-term exposure of near-infrared femtosecond laser pulses. Kinetic analysis of the intracellular accumulation of trypan blue and the assay of caspase activity revealed that femtosecond laser pulses induced immediate disturbance of plasma membrane integrity followed by apoptosis-like cell death. Yet, adjacent cells showed no sign of membrane damage and no increased caspase activity. The laser-exposed cells eventually detached from the substrate after a delay of >54 min while adjacent cells remained intact. On the base of in vitro experiments, we applied the same approach to ablate targeted single cardiac cells of a live zebrafish heart. The ability of inducing targeted cell death with femtosecond laser pulses should find broad applications that benefit from precise cellular manipulation at the level of single cells in vivo and in vitro .

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Section: Introductionmentioning

confidence: 99%

Selective induction of targeted cell death and elimination by near-infrared femtosecond laser ablation

Okano

Wang

Hong

et al. 2020

Biochemistry and Biophysics Reports

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“…As a result, when the femtosecond laser is focused on the tissue with suitable irradiation conditions, we can expect that the tissue will be stimulated mechanically, rather than thermally, by the photomechanical ablation. In previous studies we realized several single-cell manipulation techniques using shock and stress waves under a microscope, including noncontact detachment of an animal cell from its cultured substrate [11,12], control of cell multiplication [13], and estimation of intercellular adhesion strength [14,15]. These techniques can be utilized not only for single cells but also for tissues and organs of living animal.…”

Section: Introductionmentioning

confidence: 99%

New Technology: Femtosecond Laser May be Used for Future Acupuncture Therapy

Takaoka¹,

Ohta²,

Sugano³

et al. 2013

Acupuncture in Modern Medicine

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Additional information is available at the end of the chapter http://dx.doi.org/10.5772/54315. Introduction "cupuncture therapy is utilized to cure many diseases and to maintain health. We have been studying the molecular mechanism of acupuncture and obtained its molecular evidence [ -]. The World Health Organization has listed more than indications for acupuncture [ ], and the National Institutes of Health NIH has accepted the validity of acupuncture treatment [ , ]. One of the popular uses of acupuncture treatment is to treat muscle exhaustion, including stiff shoulders. Major acupuncture techniques involve penetration of the skin by thin, solid metallic needles, which are manipulated manually or are stimulated electrically. The efficacy of such muscle therapy is attributed to the overproliferation of muscle cells induced by the damage of muscle in response to the needle penetration. We recently reported that myostatin gene expression is suppressed by electroacupuncture E" [ ].However, we cannot exclude the possibility of infection for use of acupuncture, and thus a noncontact method without using needles is needed. Furthermore the laser treatment has a potential for high-throughput therapy. When an intense femtosecond laser is focused on biological tissue, laser ablation is induced at the laser focal spot. Since the main component of the tissue is water, the initiation process occurs with the mutual interaction between water molecules and the femtosecond laser pulse. In comparison with picosecond or nanosecond laser irradiation, the excitation energy of water molecules after the femtosecond laser irradiation is effectively converted to energy to generate shock and stress waves [ , ]. This means simultaneously that heat generation at the laser focal spot is suppressed because of effective energy conversion to these kinetic waves [ ]. "s a result, when the femtosecond laser is focused on the tissue with suitable irradiation conditions, we can expect that the tissue will be stimulated mechanically, rather than thermally, by the photomechanical ablation. In previous studies we realized several single-cell manipulation techniques using shock and stress waves under a microscope, including noncontact detachment of an animal cell from its cultured substrate [ , ], control of cell multiplication [ ], and estimation of intercellular adhesion strength [ , ]. These techniques can be utilized not only for single cells but also for tissues and organs of living animal. "s one of the promising approach, our attention is directed to acupuncture treatment.In this study, we focused a femtosecond laser FL on the skin of the hind leg muscles of living mice and investigated the clinical influence of the common acupuncture "CP on muscle disease via histological examination and expression analysis of the gene encoding myostatin [ ]. In the histological examination, we observed damage of the skeletal muscle due to the femtosecond laser irradiation. We then used real-time PCR to estimate the gene expression and western blotting to est...

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“…飞秒激光可以将相互作用区域限制在一个微小的三维体积内, 聚焦的飞秒激光束可以用于纳米手术, 包括对组织和细胞进行解剖 [120] 、显微外科手术 [121] , 以及转染 [122] 等. 当飞秒激光聚焦在液体中时, 会产生冲击波和空化气泡 [123] , 这一现象已经被用于聚合物的析晶 [124] 、蛋白质块和细胞的塑形 [125] 、细胞中纳米粒子的注入 [126] 、细胞的局部刺激 [127] , 和单个细胞的分离 [128] 等. 飞秒激光还可以用于眼角膜修复 [129] .…”

Section: 超快光学与超强激光技术的研究前沿unclassified