Local inflammatory reaction induced by Scolopendra viridicornis centipede venom in mice

Kimura, Louise F.; Prezotto-Neto, José Pedro; Távora, Bianca de Carvalho Lins Fernandes; Antoniazzi, Marta Maria; Knysak, Irene; Guizze, Samuel P.G.; Santoro, Marcelo Larami; Barbaro, Kátia Cristina

doi:10.1016/j.toxicon.2013.10.017

Cited by 10 publications

(7 citation statements)

References 31 publications

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“…Following these findings, Fung et al (2011) [26] reported that 40% of patients who have been admitted to Hong Kong Emergency Hospital with centipede bites (species not specified), showed an increased neutrophil-predominant leukocytosis in their blood tests with an edema and erythema at the bite site, and strong pain. We also observed that the neutrophil infiltration lasted up to 72 h, which was also reported for the crude venom of S. viridicornis [53]. Taking these observations together, the results indicate that Cryptoxin-1 may contribute to the symptoms observed in envenomation.…”

Section: Discussionsupporting

confidence: 84%

Recombinant Production and Characterization of a New Toxin from Cryptops iheringi Centipede Venom Revealed by Proteome and Transcriptome Analysis

Caetano

Nishiyama-Jr

Távora

et al. 2021

Toxins

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Among the Chilopoda class of centipede, the Cryptops genus is one of the most associated with envenomation in humans in the metropolitan region of the state of São Paulo. To date, there is no study in the literature about the toxins present in its venom. Thus, in this work, a transcriptomic characterization of the Cryptops iheringi venom gland, as well as a proteomic analysis of its venom, were performed to obtain a toxin profile of this species. These methods indicated that 57.9% of the sequences showed to be putative toxins unknown in public databases; among them, we pointed out a novel putative toxin named Cryptoxin-1. The recombinant form of this new toxin was able to promote edema in mice footpads with massive neutrophils infiltration, linking this toxin to envenomation symptoms observed in accidents with humans. Our findings may elucidate the role of this toxin in the venom, as well as the possibility to explore other proteins found in this work.

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Section: Discussionsupporting

confidence: 84%

Recombinant Production and Characterization of a New Toxin from Cryptops iheringi Centipede Venom Revealed by Proteome and Transcriptome Analysis

Caetano

Nishiyama-Jr

Távora

et al. 2021

Toxins

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“…Centipede envenomation in Brazil is frequent (Barroso et al, 2001;Medeiros et al, 2008), but underreported. In this report, it was not possible to identify the species of centipede causing the envenomation, however, those of the genus Scolopendra have the most dangerous venom to humans (Rates et al, 2007;Kimura et al, 2013).…”

Section: Discussionmentioning

confidence: 93%

“…In Brazil, three genera of centipedes are considered of medical importance: Otostigmus, Scolopendra and Cryptops (Knysak et al, 1998;Malta et al, 2008) which are responsible for human cases of envenomation, with clinical manifestations considered to be of mild severity, where pain, erythema and edema predominate (Barroso et al, 2001;Medeiros et al, 2008;Kimura et al, 2013). However, in the literature there are cases of greater severity in which the victims presented rhabdomyolysis (Logan & Ogden, 1985), lymphangitis (Essler et al, 2017), acute coronary ischemia (Ozsarac et al, 2004;Yildiz et al, 2006) and even fatal cases (Serinken et al, 2004).…”

Section: Introductionmentioning

confidence: 99%

Dermatitis Caused by Centipede Envenomation: A Case Report

Pardal¹,

Arraes²,

Weekes³

et al. 2018

Rev Patol Trop

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Accidents caused by centipedes in Brazil result in mild envenomation, where pain, erythema and edema are predominant. Cases rarely evolve to complication stages. This report presents a clinical case of dermatitis produced by an unidentified centipede, registered at the Toxicological Information Center of Belém-Pará, which evolved to blisters and necrosis. The patient underwent treatment with antibiotics and oral anti-inflammatory drugs, topical bactericides and surgical cleaning; resulting in cicatrization of the wound. The purpose of this report is to inform health professionals of the possibility of complications, which should be considered and dealt with conventionally.

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“…These mitochondrial aggregates suggest possible alterations in the overall mitochondrial function of cells (see Additional file 2). These post-exposure structural effects may be due to the release of early inflammation effectors as well as the action of enzymatic mediators that account for myotoxicity [17].…”

Section: Discussionmentioning

confidence: 99%

Mitochondrial activity disruption and local muscle damage induced in mice by Scolopendra polymorpha venom

Robles

Valverde

Cisneros

et al. 2020

J. Venom. Anim. Toxins incl. Trop. Dis

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Background: Scolopendra polymorpha (S. polymorpha) is a predatory centipede whose venom contains a multiplicity of biochemical effectors that can cause muscle damage and cumulative cell destruction in its prey. Despite previous investigations of S. polymorpha and other centipede venoms, there is a lack of information on the morphological and biochemical patterns elicited by their myotoxic effects. To elucidate these processes, this paper presents evidence of skeletal muscle damage, and alterations in key biochemical mediators that appear only after exposure to centipede venom. Methods: Venom was collected and fractionated using RP-HPLC; mouse extensor digitorum longus (EDL) muscle was exposed to whole venom and venom fractions to evaluate myotoxicity by means of creatine kinase (CK) -a muscle damage marker -activity measurements and histochemical analysis. Results: CK activity was higher in EDL muscle exposed to venom than in unexposed muscle. This increase was observed after 15 min of venom incubation, and remained stable up to 45 min. Venom-exposed EDL muscle showed signs of muscle damage including necrosis, loss of fascicular structure as well as mitochondrial accumulations and ragged red fibers (RRF), suggesting an impairment in the normal mitochondrial arrangement. Nicotinamide adenine dinucleotide (NADH) and cytochrome oxidase (COX) tests also indicate that respiratory complexes might be affected. Conclusion:Our results suggest a different biochemical composition of S. polymorpha venom, based on the different effects of four venom fractions on the cells tested, according to statistical evidence. Fractions F6 and F7 caused the most important alterations.

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Local inflammatory reaction induced by Scolopendra viridicornis centipede venom in mice

Cited by 10 publications

References 31 publications

Recombinant Production and Characterization of a New Toxin from Cryptops iheringi Centipede Venom Revealed by Proteome and Transcriptome Analysis

Recombinant Production and Characterization of a New Toxin from Cryptops iheringi Centipede Venom Revealed by Proteome and Transcriptome Analysis

Dermatitis Caused by Centipede Envenomation: A Case Report

Mitochondrial activity disruption and local muscle damage induced in mice by Scolopendra polymorpha venom

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