Local genotoxic effects of formaldehyde in humans measured by the micronucleus test with exfoliated epithelial cells

Speit, Günter; Schmid, Oliver

doi:10.1016/j.mrrev.2006.02.002

Cited by 69 publications

(51 citation statements)

References 29 publications

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“…In humans, FA exposure is associated with an increase in the frequency of MN in buccal epithelium cells (Burgaz et al, 2002;Speit et al, 2006Speit et al, , 2007b, as corroborated by the results presented here. Suruda el al.…”

Section: Discussionsupporting

confidence: 89%

Genotoxicity Biomarkers: Application in Histopathology Laboratories

Ladeira¹,

Viegas²,

Carolino³

et al. 2012

Biomarker

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Section: Discussionsupporting

confidence: 89%

Genotoxicity Biomarkers: Application in Histopathology Laboratories

Ladeira¹,

Viegas²,

Carolino³

et al. 2012

Biomarker

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“…For example, Carrasco et al (1990) found no steady correlation between abnormal variations of the erythrocyte nuclear morphology and neither (1) the measured levels of contaminants in sediments and in bile or liver of the white croaker (Genyonemus lineatus), nor (2) the prevalence of idiopathic liver lesions. Also relevant here is that variability in MN frequency exists in different studies, even with humans (Speit and Schmid 2006). So, there are inter and intraindividual variances when assessing the frequency of MN and of other nuclear anomalies, and thus there is a potential for misclassification and a need for adequate sampling.…”

Section: Introductionmentioning

confidence: 99%

Frequency of micronuclei and of other nuclear abnormalities in erythrocytes of the grey mullet from the Mondego, Douro and Ave estuaries—Portugal

Carrola

Santos

Rocha

et al. 2014

Environ Sci Pollut Res

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Fish are bioindicators of water pollution, and an increased rate of their erythrocyte nuclear morphological abnormalities (ENMAs)-and particularly of erythrocyte micronuclei (EMN)-is used as a genotoxicity biomarker. Despite the potential value of ENMAs and MN, there is scarce information about fish captured in Iberian estuaries. This is the case of the Portuguese estuaries of the Mondego, Douro and Ave, suffering from different levels of environmental stress and where chemical surveys have been disclosing significant amounts of certain pollutants. So, the aim of this study was to evaluate genotoxicants impacts and infer about the exposure at those ecosystems, using the grey mullet (Mugil cephalus) as bioindicator and considering the type and frequency of nuclear abnormalities of erythrocytes as proxies of genotoxicity. Sampling of mullets was done throughout the year in the important Mondego, Douro and Ave River estuaries (centre and north-western Portugal). The fish (total n=242) were caught in campaigns made in spring-summer and autumnwinter, using nets or fishing rods. The sampled mullets were comparable between locations in terms of the basic biometric parameters. Blood smears were stained with Diff-Quik to assess the frequencies of six types of ENMAs and MN (given per 1,000 erythrocytes). Some basic water physicochemical parameters were recorded to search for fluctuations matching the ENMAs. Overall, the most frequent nucleus abnormality was the polymorphic type, sequentially followed by the blebbed/ lobed/notched, segmented, kidney shaped, vacuolated, MN and binucleated. The total average frequency of the ENMAs ranged from 73‰ in the Mondego to 108‰ in the Ave. The polymorphic type was typically ≥50 % of the total ENMAs, averaging about 51‰, when considering all three estuaries. The most serious lesion-the MN-in fish from Mondego and Douro had a similar frequency (≈0.38‰), which was significantly lower than that in the Ave (0.75‰). No significant seasonal differences existed as to the MN rates and seasonal differences existed almost only in the Douro, with the higher values in AW. In general, the pattern of ENMAs frequencies was unrelated with the water physicochemical parameters. Considering the data for both the total ENMAs and for each specific abnormality, and bearing in mind that values of MN in fish erythrocytes >0.3‰ usually reflect pollution by genotoxicants, it is suggested that mullets were likely being chronically exposed to such compounds, even in the allegedly less polluted ecosystem (Mondego). Moreover, data supported the following pollution

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“…According to reports concerning experimental genotoxicity studies, MN are the most sensitive genetic endpoints for detection of FA induced genotoxicity [15]. Thus, MN test with exfoliated cells could be a powerful tool for detection of local genotoxic effects in humans, which is fundamental for hazard identification and risk estimation [13]. MN in peripheral blood lymphocytes has been extensively used to evaluate the presence and extend of chromosome damage in human populations exposed to genotoxic agents.…”

Section: Introductionmentioning

confidence: 99%

Genotoxic effects in occupational exposure to formaldehyde: A study in anatomy and pathology laboratories and formaldehyde-resins production

Viegas¹,

Ladeira²,

Nunes³

et al. 2010

Toxicology Letters

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Background: According to the Report on Carcinogens, formaldehyde ranks 25 th in the overall U.S. chemical production, with more than 5 million tons produced each year. Given its economic importance and widespread use, many people are exposed to formaldehyde environmentally and/or occupationally. Presently, the International Agency for Research on Cancer classifies formaldehyde as carcinogenic to humans (Group 1), based on sufficient evidence in humans and in experimental animals. Manyfold in vitro studies clearly indicated that formaldehyde can induce genotoxic effects in proliferating cultured mammalian cells. Furthermore, some in vivo studies have found changes in epithelial cells and in peripheral blood lymphocytes related to formaldehyde exposure.

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Local genotoxic effects of formaldehyde in humans measured by the micronucleus test with exfoliated epithelial cells

Cited by 69 publications

References 29 publications

Genotoxicity Biomarkers: Application in Histopathology Laboratories

Genotoxicity Biomarkers: Application in Histopathology Laboratories

Frequency of micronuclei and of other nuclear abnormalities in erythrocytes of the grey mullet from the Mondego, Douro and Ave estuaries—Portugal

Genotoxic effects in occupational exposure to formaldehyde: A study in anatomy and pathology laboratories and formaldehyde-resins production

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