LOCAL FEATURES AND PHENOTYPES OF ANTIBIOTIC RESISTANCE OF <i>PSEUDOMONAS AERUGINOSA</i>

Abstract

Objective : to estimate the resistance prevalence of P. aeruginosa to antipseudomonal antibiotics and reveal metallo-beta-lactamases producers among carbapenem resistant clinical isolates. Materials and methods. The subject of the study is clinical isolates of P. aeruginosa resistant to antibacterial drugs. The resistance of P. aeruginosa clinical isolates to antipseudomonal antibiotics was estimated, antimicrobial resistance phenotypes and associated resistance variants were analyzed, and production of metallo-beta-lactamases was detected using phenotypical screening method. Results. High levels of P. aeruginosa resistance to the most of antibacterial drugs, except carbapenems, were revealed. The local features of antimicrobial resistance in different hospitals were established. A total of 19 metallo-beta-lactamase producers were isolated in 6 hospitals of three republican regions. All metallo-beta-lactamase producers have the same phenotype, indicating of clonal prevalence. No carbapenem resistant isolates was resistant to colistin. Conclusion. It is necessary to carry out multi-facet research aimed at the detection of carbapenem resistance mechanisms and epidemiological marking of resistant isolates in order to reveal epidemically significant P.aeruginosa metallo-beta-lactamase producing clones in time and to develop the infection control measures to restrain its circulation.