Local expressions and function of Kiss1/GPR54 in goats' testes

Han, Yilin; Peng, Xiaoli; Si, Weijiang; Liu, Guiqiong; Han, Yaling; Jiang, Xunping; Na, Risu; Yang, Liguo; Wu, Jiayuan; Guang‐Xin, E; Zeng, Yan; Zhao, Yongju; Huang, Yongfu

doi:10.1016/j.gene.2020.144488

Cited by 8 publications

(12 citation statements)

References 26 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In support to IP experiments, phalloidin staining clearly showed a strong increase of the signal after Kp-10 stimulation in Leydig cells; this effect was counteracted when Leydig cells were treated with Kp-10 in combination with Kp-234. The responsiveness of Leydig cells to Kp-10 stimulation is in accordance with previous studies that showed i) the expression of Kp system, including KISS1R, in Leydig cells and ii) the increase of Kiss1/KISS1R expression in Leydig cells following Kp-10 treatment (Han et al, 2020). In addition, the reduction of TT levels following Kp-10 stimulation, associated with CYP19 increase, strongly suggested an enhanced testosterone-to-estrogen conversion, thus confirming the primary role of the Kp/KISS1R system in the regulation of Leydig cell biological functions.…”

Section: Discussionsupporting

confidence: 92%

“…The role of Kp/KISS1R system in the testis, especially in Leydig cells, has been deeply suggested ( Anjum et al, 2012 ; Chianese et al, 2013 , 2015 , 2017 ; Salehi et al, 2015 ; Han et al, 2020 ; Meccariello et al, 2020 ; Gloria et al, 2021 ).…”

Section: Discussionmentioning

confidence: 99%

“…In particular, progressive Kp expression during mouse pubertal development, especially in Leydig cells, confirms a testicular local effect of Kps. In addition, isolated Leydig cells, in vitro treated with Kps, show enhanced KISS1/KISS1R gene expression and testosterone secretion, suggesting an autocrine role of the Kp/KISS1R system in these cells ( Han et al, 2020 ).…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

KISS1R and ANKRD31 Cooperate to Enhance Leydig Cell Gene Expression via the Cytoskeletal-Nucleoskeletal Pathway

Ricci

Guillou

Catizone

et al. 2022

Front. Cell Dev. Biol.

View full text Add to dashboard Cite

Kisspeptins are involved in the regulation of hypothalamic-pituitary-gonadal axis, Leydig cell functions, and testosterone secretion, acting as endogenous ligands of the KISS1 receptor. ANKRD31 protein participates in male fertility, regulating meiotic progression, and epididymal sperm maturation. Here, we show that in Leydig cells, KISS1 receptor and ANKRD31 proteins physically interact; the formation of this protein complex is enhanced by Kisspeptin-10 that also modulates F-actin synthesis, favoring histone acetylation in chromatin and gene expression via the cytoskeletal–nucleoskeletal pathway. Kp/KISS1R system deregulation, expression impairment of cytoskeletal–nucleoskeletal mediators, Leydig gene targets, and the decreased testosterone secretion in Ankrd31−/− testis strongly supported our hypothesis. Furthermore, cytochalasin D treatment subverted the gene expression induction dependent on Kisspeptin-10 action. In conclusion, the current work highlights a novel role for the Kisspeptin-10 in the induction of the cytoskeletal–nucleoskeletal route, downstream a physical interaction between KISS1 receptor and ANKRD31, with gene expression activation as final effect, in Leydig cells.

show abstract

Section: Discussionsupporting

confidence: 92%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

KISS1R and ANKRD31 Cooperate to Enhance Leydig Cell Gene Expression via the Cytoskeletal-Nucleoskeletal Pathway

Ricci

Guillou

Catizone

et al. 2022

Front. Cell Dev. Biol.

View full text Add to dashboard Cite

show abstract

“…As the gatekeeper in HPG axis, GnRH induces the production of sex steroid by Leydig cells and the processes of spermatogenesis through LH and FSH released by the pituitary. Many studies have demonstrated the distribution of kisspeptin and its receptor proteins in the testes in multiple species including non-mammalian vertebrates such as frogs, fish and mammals such as rodents, goats and monkeys (21,(139)(140)(141). The major producer of kisspeptin is considered to be Leydig cell since scientists have detected the substantial expression of Kiss1 mRNA or protein in Leydig cells in various mammalian and non-mammalian species such as mice, horses, goats and frogs (141)(142)(143)(144).…”

Section: Kisspeptin and Male Reproductionmentioning

confidence: 99%

“…Many studies have demonstrated the distribution of kisspeptin and its receptor proteins in the testes in multiple species including non-mammalian vertebrates such as frogs, fish and mammals such as rodents, goats and monkeys ( 21 , 139 – 141 ). The major producer of kisspeptin is considered to be Leydig cell since scientists have detected the substantial expression of Kiss1 mRNA or protein in Leydig cells in various mammalian and non-mammalian species such as mice, horses, goats and frogs ( 141 – 144 ). Besides, kisspeptins were also expressed in epididymis.…”

Section: The Role Of Kisspeptin In Reproductionmentioning

confidence: 99%

The Role of Kisspeptin in the Control of the Hypothalamic-Pituitary-Gonadal Axis and Reproduction

et al. 2022

View full text Add to dashboard Cite

The discovery of kisspeptin as a critical central regulatory factor of GnRH release has given people a novel understanding of the neuroendocrine regulation in human reproduction. Kisspeptin activates the signaling pathway by binding to its receptor kisspeptin receptor (KISS1R) to promote GnRH secretion, thereby regulating the hypothalamic-pituitary-gonadal axis (HPG) axis. Recent studies have shown that kisspeptin neurons located in arcuate nucleus (ARC) co-express neurokinin B (NKB) and dynorphin (Dyn). Such neurons are called KNDy neurons. KNDy neurons participate in the positive and negative feedback of estrogen to GnRH secretion. In addition, kisspeptin is a key factor in the initiation of puberty, and also regulates the processes of female follicle development, oocyte maturation, and ovulation through the HPG axis. In male reproduction, kisspeptin also plays an important role, getting involved in the regulation of Leydig cells, spermatogenesis, sperm functions and reproductive behaviors. Mutations in the KISS1 gene or disorders of the kisspeptin/KISS1R system may lead to clinical symptoms such as idiopathic hypogonadotropic hypogonadism (iHH), central precocious puberty (CPP) and female infertility. Understanding the influence of kisspeptin on the reproductive axis and related mechanisms will help the future application of kisspeptin in disease diagnosis and treatment. In this review, we critically appraise the role of kisspeptin in the HPG axis, including its signaling pathways, negative and positive feedback mechanisms, and its control on female and male reproduction.

show abstract