Local elastic properties of cells studied by SFM

Lekka, Małgorzata; Lekki, Janusz; Marszałek, M.; Golonka, P; Stachura, Z.; Cleff, B.; Hrynkiewicz, A.Z.

doi:10.1016/s0169-4332(98)00522-4

Cited by 45 publications

(33 citation statements)

References 6 publications

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“…Expression and purification of AFM imaging was performed using a home-built device [42] equipped with a liquid cell [43] and working in a contact mode and using Agilent N9410S -Series 5500 AFM/SPM System. Samples were scanned in buffer at ambient temperature, at line frequencies of 2.5-3.…”

Section: Methodsmentioning

confidence: 99%

Global architecture of human poly(A)-specific ribonuclease by atomic force microscopy in liquid and dynamic light scattering

Niedzwiecka

Lekka

Nilsson

et al. 2011

Biophysical Chemistry

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Deadenylation is the initial and often rate-limiting step in the main pathways of eukaryotic mRNA decay. Poly(A)-specific ribonuclease (PARN) is a eukaryotic enzyme that efficiently degrades mRNA poly(A) tails. Structural and functional studies have shown that human PARN is composed of at least three functional domains, i.e. the catalytic nuclease domain and two RNA binding domains, the R3H and the RNA recognition motif (RRM), respectively. However, the complete structure of the full length protein is still unknown. We have investigated the global architecture of human PARN by atomic force microscopy (AFM) imaging in buffered milieu and report for the first time the dimensions of the full length protein at subnanometre resolution. The AFM images of single PARN molecules reveal compact ellipsoidal dimers (10.9 × 7.6 × 4.6 nm). The dimeric form of PARN was confirmed by dynamic light scattering (DLS) measurements that rendered a molecular weight of 161 kDa, in accordance with previous crystal structures of PARN fragments showing a dimeric composition. We discuss a putative internal arrangement of three functional domains within the full length PARN dimer.

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Section: Methodsmentioning

confidence: 99%

Global architecture of human poly(A)-specific ribonuclease by atomic force microscopy in liquid and dynamic light scattering

Niedzwiecka

Lekka

Nilsson

et al. 2011

Biophysical Chemistry

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“…The AFM, used for the measurements, was a home--built device, which was described in detail elsewhere [10]. It is equipped with a "liquid cell" setup made of plexiglass.…”

Section: Atomic Force Microscopementioning

confidence: 99%

Energy Dissipation in the AFM Elasticity Measurements

et al. 2009

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Nowadays, it is well established that changes of cell stiffness observed by atomic force microscopy are linked with the cell cytoskeleton. Its structural and functional alterations are underlying major diseases such as cancer, inflammation or neurodegenerative disorders. So far, the use of atomic force microscopy is mostly focused on the determination of the Young modulus using the modified Hertz model. It can quantitatively describe the elastic properties of living cells, however, its value is burdened by the fact that cells are neither isotropic nor homogeneous material. Often, during the atomic force microscopy measurements, the hysteresis between the loading and unloading curves are observed which indicates the dissipation of an energy. In our studies, the index of plasticity was introduced to enumerate such effect during a single loading-unloading cycle. As the results show, such approach delivers an additional parameter describing the mechanical state of cell cytoskeleton. The analysis was performed on test samples where the mechanical properties of the melanoma cells were changed by glutaraldehyde and cytochalasin D treatments. The non-treated cells were compared with fibroblasts.

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“…The organization of the cell's exterior ''sensing elements'' and other specialized regions of the membrane is tailored to reflect the function of the cell and serves vital roles in cell-cell interactions, cell signaling, and cell-surface interactions. The changes that occur in these important chemical͞mechanical phenotypes during the development of cancer and other diseases may be understood in much more detail, thereby allowing the relationships between specific phenotypes to cell and tissue normo-and pathophysiology, prognosis, and therapy to be discerned (1). Recent studies have shown that the components that comprise the membrane are segregated into domains that are dynamic and change in response to external and internal stimuli (2)(3)(4)(5).…”

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confidence: 99%

Combining constitutive materials modeling with atomic force microscopy to understand the mechanical properties of living cells

McElfresh

Baesu

Balhorn

et al. 2002

Proc. Natl. Acad. Sci. U.S.A.

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The goal of this work is to study the properties of living cells and cell membranes by using atomic force microscopy. During atomic force microscopy (AFM) measurement, there is a strong mechanical coupling between the AFM tip and the cell. The purpose of this paper is to present a model of the overall mechanical response of the cell that allows us to separate out the mechanical response of the cell from the local surface interactions we wish to quantify. These local interactions include recognition (or binding) events between molecules bound to an AFM tip (e.g., an antibody) and molecules or receptors on the cell surface (e.g., the respective antigen). The computational model differs from traditional Hertzian contact models by explicitly taking into account the mechanics of the biomembrane and cytoskeleton. The model also accounts for the mechanical response of the living cell during arbitrary deformation. The indentation of a bovine sperm cell is used to test the validity of this model, and further experiments are proposed to fully parameterize the model.

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Local elastic properties of cells studied by SFM

Cited by 45 publications

References 6 publications

Global architecture of human poly(A)-specific ribonuclease by atomic force microscopy in liquid and dynamic light scattering

Global architecture of human poly(A)-specific ribonuclease by atomic force microscopy in liquid and dynamic light scattering

Energy Dissipation in the AFM Elasticity Measurements

Combining constitutive materials modeling with atomic force microscopy to understand the mechanical properties of living cells

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