This paper develops a spatial input-output approach to investigate the dynamics of a turbulent boundary layer subject to a localized single frequency excitation. This method uses one-way spatial integration to reformulate the problem in terms of spatial evolution equations. The technique is used to examine the effect of localized periodic actuation at a given temporal frequency, based on an experimental set-up in which an active large-scale is introduced into the outer layer of a turbulent boundary layer. First, the large-scale structures associated with the phase-locked modal velocity field obtained from spatial input-output analysis are shown to closely match those computed based on hot-wire measurements. The approach is then used to further investigate the response of the boundary layer to the synthetically generated large-scale. A quadrant trajectory analysis indicates that the spatial input-output response produces shear stress distributions consistent with those in canonical wall-bounded turbulent flows in terms of both the order and types of events observed. The expected correspondence between the dominance of different quadrant behavior and actuation frequency is also observed. These results highlight the promise of a spatial input-output framework for analyzing the formation and streamwise evolution of structures in actuated wall-bounded turbulent flows. Nomenclature 𝑥 = streamwise location 𝑦 = wall-normal location 1 Graduate student, Department of Mechanical Engineering.