Local database and the search program for proteomic analysis of sperm proteins in the ascidian Ciona intestinalis

Hozumi, Akiko; Satouh, Yuhkoh; Ishibe, Daisuke; Kaizu, Maiko; Konno, Aru; Ushimaru, Yuji; Toda, Tosifusa; Inaba, Kazuo

doi:10.1016/j.bbrc.2004.05.118

Cited by 27 publications

(33 citation statements)

References 7 publications

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“…This information has been used to perform proteomic approaches in this organism [20]. For example, the proteins constituting sperm were analyzed using two-dimensional electrophoresis tagged with mass spectrometry, and the proteins showing changes during sperm activation were identified [16]. Ciona has another advantage for the use of genetic approaches in that its generation time is approximately 2-3 months.…”

Section: Ciona Intestinalis As Subjects In the Study Of Genetic Functmentioning

confidence: 99%

Ciona intestinalis and Oxycomanthus japonicus, Representatives of Marine Invertebrates

et al. 2009

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Abstract:The study of marine invertebrates is useful in various biological research fields. However, genetic analyses of these animals are limited, mainly due to difficulties in culturing them, and the genetic resources of marine invertebrates have not been organized. Recently, advances have been made in the study of two deuterostomes, an ascidian Ciona intestinalis and a feather star Oxycomanthus japonicus. The draft genome sequence of Ciona intestinalis has been determined, and its compact genome, which has less redundancy of genes compared with vertebrates, provides us with a useful experimental system for analyzing the functions of genes during development. The life cycle of Ciona intestinalis is approximately 2-3 months, and the genetic techniques including a perfect inland culture system, germline transformation with a transposon Minos, enhancer detection and insertional mutagenesis, have been established. The feather star Oxycomanthus japonicus conserves the characteristics of the basic echinoderm body plan with a segmented mesoderm, which is a fascinating characteristic for understanding the evolution of echinoderms. Oxycomanthus japonicus shows strong regeneration ability and is a suitable subject for analysis of the mechanisms of regeneration. In consideration of these features, the National BioResource Project (NBRP) has started to support the supply of wild-types, transgenic lines and inbred lines of Ciona intestinalis and Oxycomanthus japonicus.

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Section: Ciona Intestinalis As Subjects In the Study Of Genetic Functmentioning

confidence: 99%

Ciona intestinalis and Oxycomanthus japonicus, Representatives of Marine Invertebrates

et al. 2009

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“…By using peptide mass fingerprinting (PMF) system for Ciona testis [Hozumi et al, 2004], all of these proteins have been identified, including axonemal and non-axonemal proteins.…”

Section: Introductionmentioning

confidence: 99%

Molecular characterization of axonemal proteins and signaling molecules responsible for chemoattractant‐induced sperm activation in Ciona intestinalis

Hozumi

Padma

Toda

et al. 2008

Cell Motil. Cytoskeleton

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Spermatozoa undergo dramatic physiological changes at fertilization. In the ascidian Ciona intestinalis, an egg-derived substance named SAAF induces both sperm activation and chemotaxis to the egg. To elucidate the molecular mechanism underlying these phenomena, whole sperm proteins before and after SAAF-treatment were analyzed by two-dimensional gel electrophoresis. By comparison of spot patterns before and after activation, we found twelve proteins that changed the isoelectric points. Seven proteins were shown to be axonemal proteins and others were suggested to be non-axonemal components. Analysis of these proteins by MS-based proteomic system revealed that components of several substructures of the axonemes underwent the changes in isoelectric point at sperm activation, including WD-repeat intermediate chains of outer and inner arm dyneins and a radial spoke protein LRR37, as well as novel axonemal proteins with armadillo repeats or SMC domain. Molecules for cell signaling such as 14-3-3 proteins, Skp1 and VCP/p97 also showed isoelectric changes at sperm activation. These results show a comprehensive feature for signaling mechanism of the activation of spermatozoa at fertilization and also shed new lights on the regulation of ciliary and flagellar movements.

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“…The mature male germ cell proteome has been studied in model species such as plants [22] or worms [23]. In man, specific approaches to target subcellular organelles or compartments have been successfully used [11,[24][25][26][27][28].…”

Section: Introductionmentioning

confidence: 99%

Proteomic identification of human sperm proteins

Martínez-Heredia¹,

Estanyol²,

Ballescà³

et al. 2006

Proteomics

230

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Conventional 1-DE has in the past provided a wealth of information concerning the major sperm proteins. However, so far there are relatively few reports exploiting the potential of the present proteomic tools to identify and to study additional yet-unidentified important proteins present in human spermatozoa. In the present work, 2-DE of proteins extracted from human normozoospermic spermatozoa led to the resolution of over 1000 spots. Subsequent excision from the gels of 145 spots and MALDI-TOF MS analysis allowed the identification of 98 different proteins. The function of these proteins turned out to be energy production (23%), transcription, protein synthesis, transport, folding and turnover (23%), cell cycle, apoptosis and oxidative stress (10%), signal transduction (8%), cytoskeleton, flagella and cell movement (10%), cell recognition (7%), metabolism (6%) and unknown function (11%). As many as 23% of the proteins identified have not been previously described as being expressed in human spermatozoa. The present data provide an important clue towards determining the function of these proteins and opens up the possibility to perform additional experiments.

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Local database and the search program for proteomic analysis of sperm proteins in the ascidian Ciona intestinalis

Cited by 27 publications

References 7 publications

Ciona intestinalis and Oxycomanthus japonicus, Representatives of Marine Invertebrates

Ciona intestinalis and Oxycomanthus japonicus, Representatives of Marine Invertebrates

Molecular characterization of axonemal proteins and signaling molecules responsible for chemoattractant‐induced sperm activation in Ciona intestinalis

Proteomic identification of human sperm proteins

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