Local cobalt injection: a method to discriminate presynaptic axonal from postsynaptic neuronal activity

Kretz, Robert

doi:10.1016/0165-0270(84)90030-x

Cited by 120 publications

(88 citation statements)

References 13 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…In the present study, LH-induced antinociception was blocked by administration of cobalt chloride in a dose shown to block synaptic activity in the area of microinjection (Kretz, 1984;Nuseir et al, 1999;Holden et al, 2002). This finding is suggestive that unidentified LH neurons send axons to the RVM and activate serotonergic neurons that then alter nociceptive responses in the spinal cord dorsal horn.…”

Section: Discussionsupporting

confidence: 72%

Lateral hypothalamic-induced antinociception may be mediated by a substance P connection with the rostral ventromedial medulla

Holden

Pizzi

2008

Brain Research

View full text Add to dashboard Cite

Section: Discussionsupporting

confidence: 72%

Lateral hypothalamic-induced antinociception may be mediated by a substance P connection with the rostral ventromedial medulla

Holden

Pizzi

2008

Brain Research

View full text Add to dashboard Cite

“…CoCl 2 injections have also been used to block transmission in cardiovascular pathways with a similar time course to that observed in this study (2,13,20,26,27,30). This interruption of neuronal transmission with CoCl 2 has been shown to be due to a reversible inactivation of calcium channels on the presynaptic terminals (18,22,23).…”

Section: Discussionmentioning

confidence: 93%

“…Glu was chosen as it is well known to selectively excite neuronal cell bodies and not fibers of passage (14). In addition, studies were done to determine whether these effects were mediated in part through an obligatory synapse in the NTS using CoCl 2 injections into NTS (18).…”

mentioning

confidence: 99%

Medullary pathways mediating the parasubthalamic nucleus depressor response

Ciriello¹,

Solano-Flores²,

Rosas‐Arellano³

et al. 2008

American Journal of Physiology-Regulatory, Integrative and Comp

View full text Add to dashboard Cite

The parasubthalamic nucleus (PSTN) projects extensively to the nucleus of the solitary tract (NTS); however, the function of PSTN in cardiovascular regulation is unknown. Experiments were done in ␣-chloralose anesthetized, paralyzed, and artificially ventilated rats to investigate the effect of glutamate (10 nl, 0.25 M) activation of PSTN neurons on mean arterial pressure (MAP), heart rate (HR), and renal sympathetic nerve activity (RSNA). Glutamate stimulation of PSTN elicited depressor (Ϫ20.4 Ϯ 0.7 mmHg) and bradycardia (Ϫ26.0 Ϯ 1.0 beats/min) responses and decreases in RSNA (67 Ϯ 17%). Administration (intravenous) of atropine methyl bromide attenuated the bradycardia response (46%), but had no effect on the MAP response. Subsequent intravenous administration of hexamethonium bromide blocked both the remaining bradycardia and depressor responses. Bilateral microinjection of the synaptic blocker CoCl 2 into the caudal NTS region attenuated the PSTN depressor and bradycardia responses by 92% and 94%, respectively. Additionally, prior glutamate activation of neurons in the ipsilateral NTS did not alter the magnitude of the MAP response to stimulation of PSTN, but potentiated HR response by 35%. Finally, PSTN stimulation increased the magnitude of the reflex bradycardia to activation of arterial baroreceptors. These data indicate that activation of neurons in the PSTN elicits a decrease in MAP due to sympathoinhibition and a cardiac slowing that involves both vagal excitation and sympathoinhibition. In addition, these data suggest that the PSTN depressor effects on circulation are mediated in part through activation of NTS neurons involved in baroreflex function. lateral hypothalamus; blood pressure; bradycardia; baroreceptor reflex; nucleus of the solitary tract

show abstract

“…The technique is based on administering circumscribed microinjections of compounds that block neuronal activity. Microinjection of cobalt into brain areas has been used for functional inactivation (Kretz, 1984) because it prevents the release of synaptic transmitters by blocking calcium channels in the presynaptic membrane (Hagiwara and Byerly, 1981). Cobalt is a more effective inhibitor in the central nervous system than local anesthetics or specific receptor agonists.…”

Section: T R a C Tmentioning

confidence: 99%

Involvement of the paraventricular nucleus (PVN) of hypothalamus in the cardiovascular alterations to head up tilt in conscious rats

Andrade

Karlen‐Amarante

Bellinati

et al. 2012

Neuroscience Research

View full text Add to dashboard Cite

Local cobalt injection: a method to discriminate presynaptic axonal from postsynaptic neuronal activity

Cited by 120 publications

References 13 publications

Lateral hypothalamic-induced antinociception may be mediated by a substance P connection with the rostral ventromedial medulla

Lateral hypothalamic-induced antinociception may be mediated by a substance P connection with the rostral ventromedial medulla

Medullary pathways mediating the parasubthalamic nucleus depressor response

Involvement of the paraventricular nucleus (PVN) of hypothalamus in the cardiovascular alterations to head up tilt in conscious rats

Contact Info

Product

Resources

About