Local cerebral glucose utilization in the free moving mouse: a comparison during two stages of the activity-rest cycle

Jay, Thérèse M.; Jouvet, Michel; Rosiers, M.H. Des

doi:10.1016/0006-8993(85)91129-1

Cited by 38 publications

(28 citation statements)

References 26 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…LCGU during static and low-intensity dynamic arm exercise has been studied in monkeys, and for both types of ex ercise, increases in LCGU were observed in senso rimotor cortex in the hemisphere contralateral to the moving forelimb (Schwartzman et al, 1981;Sa vaki et al, 1993). LCG U has also been studied in mice during two stages of the light-dark cycle in which the mice are "drowsy" and "active," re spectively (Jay et al, 1985). In parallel with findings in the present study, LCGU increased in active ver sus drowsy mice in the thalamus, amygdaloid n., and sensorimotor and auditory cortex.…”

Section: Discussionsupporting

confidence: 87%

Exercise-Induced Changes in Local Cerebral Glucose Utilization in the Rat

Vissing

Andersen²,

Diemer

1996

J Cereb Blood Flow Metab

145

View full text Add to dashboard Cite

Summary: In exercise, little is known about local cere bral glucose utilization (LCGU), which is an index of functional neurogenic activity. We measured LCGU in resting and running (=85% of maximum O2 uptake) rats (n = 7 in both groups) previously equipped with a tail artery catheter. LCGU was measured quantitatively from 2-deoxY-D-[1-14C]glucose autoradiographs. During exer cise, total cerebral glucose utilization (TCGU) increased by 38% (p < 0.005). LCGU increased (p < 0.05) in areas involved in motor function (motor cortex 39%, cerebel lum = 110%, basal ganglia =30%, substantia nigra =37%, and in the following nuclei: subthalamic 47%, posterior hypothalamic 74%, red 61%, ambiguus 43%, pontine 61 %), areas involved in sensory function (somatosensory 27%, auditory 32%, and visual cortex 42%, thalamus =75%, and in the following nuclei: Darkschewitsch 22%, cochlear 51%, vestibular 30%, superior olive 23%, cuPronounced motor, sensory, and autonomic ad aptation is known to occur in response to both static and dynamic exercise. Such changes must be thought to evoke marked regional changes in cere bral functional activity, but little is known about the effect of exercise on regional cerebral blood flow and in particular on regional cerebral metabolism, which are both indexes of the functional activity of regional neurons (Sokoloff, 1991(Sokoloff, , 1992 729neate 115%), areas involved in autonomic function (dor sal raphe nucleus 30%, and areas in the hypothalamus =35%, amygdala =35%, and hippocampus 29%), and in white matter of the corpus callosum (36%) and cerebel lum (52%). LCGU did not change with exercise in pre frontal and frontal cortex, cingulum, inferior olive, nu cleus of solitary tract and median raphe, lateral septal and interpenduncular nuclei, or in areas of the hippocampus, amygdala, and hypothalamus. Glucose utilization did not decrease during exercise in any of the studied cerebral regions. In summary, heavy dynamic exercise increases TCGU and evokes marked differential changes in LCGU. The findings provide clues to the cerebral areas that par ticipate in the large motor, sensory, and autonomic adap tation occurring in exercise. Key Words: Autoradiogra phy-Brain glucose metabolism-2-Deoxyglucose.

show abstract

Section: Discussionsupporting

confidence: 87%

Exercise-Induced Changes in Local Cerebral Glucose Utilization in the Rat

Vissing

Andersen²,

Diemer

1996

J Cereb Blood Flow Metab

145

View full text Add to dashboard Cite

show abstract

“…Therefore, it is highly unlikely that the relative changes in CMR glc between fmr1 KO and WT mice reported in our study are due to any methodological issues. Our values for CMR glc in WT mice are in good agreement with those reported previously in Swiss albino mice (38)(39)(40) but are lower than values reported in CFW (41) and C57BL͞6J mice (42,43). Whether this disparity is a function of the strain studied or a difference in procedure remains to be determined.…”

Section: Discussionsupporting

confidence: 80%

Increased rates of cerebral glucose metabolism in a mouse model of fragile X mental retardation

Qin

Kang

Smith

2002

Proc. Natl. Acad. Sci. U.S.A.

View full text Add to dashboard Cite

In humans, failure to express the fragile X mental retardation protein (FMRP) gives rise to fragile X syndrome, the most common form of inherited mental retardation. A fragile X knockout (fmr1 KO) mouse has been described that has some of the characteristics of patients with fragile X syndrome, including immature dendritic spines and subtle behavioral deficits. In our behavioral studies, fmr1 KO mice exhibited hyperactivity and a higher rate of entrance into the center of an open field compared with controls, suggesting decreased levels of anxiety. Our finding of impaired performance of fmr1 KO mice on a passive avoidance task is suggestive of a deficit in learning and memory. In an effort to understand what brain regions are involved in the behavioral abnormalities, we applied the [ 14 C]deoxyglucose method for the determination of cerebral metabolic rates for glucose (CMR glc). We measured CMRglc in 38 regions in adult male fmr1 KO and WT littermates. We found CMR glc was higher in all 38 regions in fmr1 KO mice, and in 26 of the regions, differences were statistically significant. Differences in CMR glc ranged from 12% to 46%, and the greatest differences occurred in regions of the limbic system and primary sensory and posterior parietal cortical areas. Regions most affected are consistent with behavioral deficiencies and regions in which FMRP expression is highest. Higher CMR glc in fragile X mice may be a function of abnormalities found in dendritic spines.

show abstract

“…In each group, 16 animals were randomized to be used either for the autoradiographic determination of local CBF (n = 8) or for the measurement of local CMR glc (n = 8), according to the methods described in the rat (Sakurada et al, 1978;Sokoloff et al, 1977). These autoradiographic methods were adapted to mice according to the previous use in this species (Jay et al, 1985(Jay et al, , 1988.…”

Section: Measurement Of Local Cerebral Blood Flow and Local Cerebral mentioning

confidence: 99%

Reduced Cerebral Blood Flow but Elevated Cerebral Glucose Metabolic Rate in Erythropoietin Overexpressing Transgenic Mice with Excessive Erythrocytosis

Frietsch

Maurer

Vogel

et al. 2006

J Cereb Blood Flow Metab

View full text Add to dashboard Cite

To examine the impact of excessive erythrocytosis on local cerebral blood flow (CBF) and cerebral glucose metabolic rate (CMR glc ), we made use of our constitutively erythropoietin (Epo)-overexpressing transgenic mouse line (tg-6) that reach a mean hematocrit of 0.87. Compared with wildtype (wt) control siblings, CBF decreased by 44% in tg-6 mice, while upon hemodilution (tg-6-HD) to a physiologic hematocrit (e.g., 0.44) tg-6-HD mice returned the CBF to wt levels. Cerebral blood flow was determined in another transgenic mouse line that overexpresses human Epo in the brain only (tg-21): CBF increased by 17% compared with wt controls. However, oxygen delivery was similar in all four mouse groups tested (wt, tg-6, tg-6-HD and tg-21). Mean CMR glc was higher in tg-6 ( + 72%), tg-6-HD mice ( + 43%) and tg-21 ( + 22%) than in wt mice. Local CMR glc was higher in all 40 brain regions in tg-6 but only in 15 and 8 regions in tg-6-HD and tg-21 mice. These results show that prolonged increases in hematocrit did not alter cerebral oxygen delivery at a decreased CBF and increased CMR glc . Hemodilution suggests that high blood viscosity is a cause of the decrease in CBF and partly of the increase in CMR glc . Cerebral glucose metabolic rate may also be increased by a direct effect of Epo in the brain (tg-21 mice).

show abstract

Local cerebral glucose utilization in the free moving mouse: a comparison during two stages of the activity-rest cycle

Cited by 38 publications

References 26 publications

Exercise-Induced Changes in Local Cerebral Glucose Utilization in the Rat

Exercise-Induced Changes in Local Cerebral Glucose Utilization in the Rat

Increased rates of cerebral glucose metabolism in a mouse model of fragile X mental retardation

Reduced Cerebral Blood Flow but Elevated Cerebral Glucose Metabolic Rate in Erythropoietin Overexpressing Transgenic Mice with Excessive Erythrocytosis

Contact Info

Product

Resources

About