Local carotid atherosclerotic plaque proteins for the identification of circulating biomarkers in coronary patients

Higher Serum Levels of Osteoglycin Are Associated with All-Cause Mortality and Cardiovascular and Cerebrovascular Events in Patients with Advanced Chronic Kidney Disease

“…OGN belongs to the family of small leucine-rich proteoglycans and is a basic component of the vascular extracellular matrix (Shanahan et al 1997;Iozzo 1999;Cheng et al 2014;Van Aelst et al 2015). Recently, serum OGN has been shown to be released by vulnerable hemorrhagic carotid and coronary atherosclerotic plaques (Cheng et al 2014;Malaud et al 2014) and may also have prognostic value in patients with coronary artery disease (Cheng et al 2014). Furthermore, OGN level was found to be increased in the adventitia and neointima after balloon injury, suggesting a role in vessel matrix remodeling (Shanahan et al 1997).…”

Circulating Osteoglycin and NGAL/MMP9 Complex Concentrations Predict 1-Year Major Adverse Cardiovascular Events After Coronary Angiography

“…16 In our previous proteomics experiments, however, OGN was found to be downregulated in hemorrhagic plaques compared with fibrotic plaques. 8 The lower OGN levels suggested an altered strength and integrity of the vascular wall, which may make hemorrhagic plaques more prone to rupture. Its role as a prognostic circulating biomarker has also not been investigated yet.…”

“…8 We used human carotid atherosclerotic plaques, obtained from patients who underwent endarterectomy (n=80), which were classified as fibrotic plaques or hemorrhagic plaques according to the AHA classification and hemoglobin content. 8,11,12 We performed protein enrichment and mass spectrometry analysis and subsequently validation by Western blotting. We found that several tissue proteins within human atherosclerotic plaques were able to differentiate between vulnerable, hemorrhagic and stable, fibrolipidic lesions.…”

“…7 In a previous proteomic study, we have found several proteins to be differentially released by vulnerable hemorrhagic human carotid plaques when compared with stable fibrotic plaques. 8 The majority of these proteins, including aciculin, oncogene DJ1 (DJ1), microfibril-associated glycoprotein 4, osteoglycin (OGN), procollagen C proteinase enhancer 1, phosphatidylethanol-amine-binding protein 1, and peroxiredoxin 2, have not yet been investigated as prognostic biomarkers in CAD patients. In addition, evidence exists that neutrophil gelatinase-associated lipocalin (NGAL) and its NGAL/matrix metalloproteinase 9 (NGAL/MMP9) complex display increased expression in (unstable) atherosclerotic plaques.…”

Common Mechanisms of Onset of Cancer and Neurodegenerative Diseases

“…131,133) Indeed, recent studies have shown relationships of DJ-1 with glucose, hypertension and obesity markers. 165,[177][178][179][180][181][182][183] As for Parkinson's disease, dopamine is synthesized from tyrosine by two enzymes, tyrosine hydroxylase (TH) and dopamine decarboxylase (DDC), and packed into vesicles by vesicular monoamine transporter 2 (VMAT2). Expression of the TH gene is negatively regulated by PSF, a repressor of transcription.…”