Local brassinosteroid biosynthesis enables optimal root growth

Vukašinović, Nemanja; Wang, Yaowei; Vanhoutte, Isabelle; Fendrych, Matyáš; Guo, Boyu; Kvasnica, Miroslav; Jiroutová, Petra; Oklešťková, Jana; Strnad, Miroslav; Russinova, Eugenia

doi:10.1038/s41477-021-00917-x

Cited by 74 publications

(104 citation statements)

References 64 publications

(78 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…It has also been proposed that BR signalling regulation of BR biosynthesis genes in the inner stele tissue modulates BR levels that are perceived in the outer tissues, thus providing a mode of inter-tissue coordination 19 . In addition, BR intermediates appear to move within the meristem 20 . However, data on how BR signalling controls geometry on a cellular scale and how it integrates to the whole-organ scale, as with radial growth of the root meristem, is lacking.…”

Section: Mainmentioning

confidence: 99%

The root meristem is shaped by brassinosteroid control of cell geometry

et al. 2021

View full text Add to dashboard Cite

Growth extent and direction determine cell and whole-organ architecture. How they are spatio-temporally modulated to control size and shape is not well known. Here we tackled this question by studying the effect of brassinosteroid (BR) signalling on the structure of the root meristem. Quantification of the three-dimensional geometry of thousands of individual meristematic cells across different tissue types showed that the modulation of BR signalling yields distinct changes in growth rate and anisotropy, which affects the time that cells spend in the meristem and has a strong impact on the final root form. By contrast, the hormone effect on cell volume was minor, establishing cell volume as invariant to the effect of BR. Thus, BR has the highest effect on cell shape and growth anisotropy, regulating the overall longitudinal and radial growth of the meristem, while maintaining a coherent distribution of cell sizes. Moving from single-cell quantification to the whole organ, we developed a computational model of radial growth. The simulation demonstrates how differential BR-regulated growth between the inner and outer tissues shapes the meristem and thus explains the non-intuitive outcomes of tissue-specific perturbation of BR signalling. The combined experimental data and simulation suggest that the inner and outer tissues have distinct but coordinated roles in growth regulation.

show abstract

Section: Mainmentioning

confidence: 99%

The root meristem is shaped by brassinosteroid control of cell geometry

et al. 2021

View full text Add to dashboard Cite

show abstract

“…The positive effect that TdPP1 exerted on root growth upon BR exposure may be due to its capacity to induce subtle changes in BR signaling in a way that helps the plant to cope with the negative effects of high BR concentrations on root growth and development. Moreover, BR biosynthesis and active BES1 nuclear accumulation optima overlap in the elongation zone, and little or no nuclear active forms of BES1 are found in the root tip [39][40][41]. By binding to and activating BES1, under control conditions, TdPP1 might slightly increase BR signaling through BES1 activation, all along the root, in a way that mimics optimal BR concentrations so that primary root growth is increased in these lines.…”

Section: Discussionmentioning

confidence: 98%

Wheat Type One Protein Phosphatase Participates in the Brassinosteroid Control of Root Growth via Activation of BES1

Bradai

Amorim‐Silva

Belgaroui

et al. 2021

IJMS

View full text Add to dashboard Cite

Brassinosteroids (BRs) play key roles in diverse plant growth processes through a complex signaling pathway. Components orchestrating the BR signaling pathway include receptors such as kinases, transcription factors, protein kinases and phosphatases. The proper functioning of the receptor kinase BRI1 and the transcription factors BES1/BZR1 depends on their dephosphorylation by type 2A protein phosphatases (PP2A). In this work, we report that an additional phosphatase family, type one protein phosphatases (PP1), contributes to the regulation of the BR signaling pathway. Co-immunoprecipitation and BiFC experiments performed in Arabidopsis plants overexpressing durum wheat TdPP1 showed that TdPP1 interacts with dephosphorylated BES1, but not with the BRI1 receptor. Higher levels of dephosphorylated, active BES1 were observed in these transgenic lines upon BR treatment, indicating that TdPP1 modifies the BR signaling pathway by activating BES1. Moreover, ectopic expression of durum wheat TdPP1 lead to an enhanced growth of primary roots in comparison to wild-type plants in presence of BR. This phenotype corroborates with a down-regulation of the BR-regulated genes CPD and DWF4. These data suggest a role of PP1 in fine-tuning BR-driven responses, most likely via the control of the phosphorylation status of BES1.

show abstract

“…The auxin gradient parallels that of the PLETHORA (PLT) proteins, maintained by their high stability but diluted over consecutive cell cycles ( Galinha et al , 2007 ; Mähönen et al , 2014 ). While these appear as the major players in establishing the root zonation, other hormones also play crucial roles, including brassinosteroids, gibberellin, ethylene, and abscisic acid ( Moubayidin et al , 2010 ; Sozzani and Iyer-Pascuzzi, 2014 ; Thole et al , 2014 ; Schaller et al , 2014 ; Jaillais and Vert, 2016 ; Birnbaum, 2016 ; Pavelescu et al , 2018 ; Vaseva et al , 2018 ; Shimotohno and Scheres, 2019 ; Vukašinović et al , 2021 ). How are these gradients of hormonal signals and downstream factors connected with the ability of cells to proliferate?…”

Section: Discussionmentioning

confidence: 99%

A perspective on cell proliferation kinetics in the root apical meristem

Desvoyes

Echevarría

Gutiérrez

2021

Journal of Experimental Botany

View full text Add to dashboard Cite

Organogenesis in plants is primarily postembryonic and relies in a strict balance between cell division and cell expansion. The root is a particularly well-suited model to study cell proliferation in detail since the two processes are spatially and temporally separated for all different tissues. In addition, the root is amenable for detailed microscopic analysis to identify cells progressing through the cell cycle. While it is clear that cell proliferation activity is restricted to the root apical meristem (RAM), the understanding of cell proliferation kinetics and identification of its parameters has been a long effort over the years. Here, we review the main concepts, experimental settings and findings aimed at obtaining a detailed knowledge of how cells proliferate within the RAM. The combination of novel tools, experimental strategies and mathematical models have contributed to our current view of cell proliferation in the RAM. Also, we discuss several lines of research that are needed to be explored in the future.

show abstract

Local brassinosteroid biosynthesis enables optimal root growth

Cited by 74 publications

References 64 publications

The root meristem is shaped by brassinosteroid control of cell geometry

The root meristem is shaped by brassinosteroid control of cell geometry

Wheat Type One Protein Phosphatase Participates in the Brassinosteroid Control of Root Growth via Activation of BES1

A perspective on cell proliferation kinetics in the root apical meristem

Contact Info

Product

Resources

About