Local Auxin Biosynthesis Mediated by a YUCCA Flavin Monooxygenase Regulates Haustorium Development in the Parasitic Plant <i>Phtheirospermum japonicum</i>

Ishida, Juliane Karine; Wakatake, Takanori; Yoshida, Satoko; Takebayashi, Yumiko; Kasahara, Hiroyuki; Wafula, Eric; dePamphilis, Claude W.; Namba, Susumu; Shirasu, Ken

doi:10.1105/tpc.16.00310

Cited by 102 publications

(145 citation statements)

References 84 publications

(113 reference statements)

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“…However, QR2 transcripts exhibited strong PhQ-coexpression in P. aegyptiaca (kPhQ =4) and S. hermonthica (kPhQ =3)( Fig. 3E), in line with QR2 responsiveness to HIFs during haustorium formation of Phtheirospermum japonicum and S. asiatica (23,24). These findings, along with the reported PM-association of AtQR2s (25), support QR2 involvement in the PM redox system.…”

supporting

confidence: 77%

Plasma Membrane Phylloquinone Biosynthesis in Nonphotosynthetic Parasitic Plants

Gu¹,

Chen²,

Nyamdari³

et al. 2018

Preprint

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Phylloquinone (PhQ) is found predominantly in thylakoid membranes of photosynthetic tissues where it functions in photosystem I electron transport. PhQ has also been detected in plasma membranes, but neither the molecular basis nor the significance of this noncanonical pathway has been elucidated. Here we provide evidence of plasma membrane PhQ biosynthesis in a non-photosynthetic holoparasite Phelipanche aegyptiaca. A nonphotosynthetic role is supported by transcription of the entire suite of PhQ biosynthetic genes, plasma membrane-localization of the terminal enzymes, and detection of PhQ in Phelipanche seeds. Analysis of PhQ-coexpressed genes in the holoparasite revealed increased cellular commitment to oxidation-reduction and defense relative to photosynthetic parasites. Coexpression network inference identified oxidoreductases involved in plasma membrane electron transport, implicating PhQ in a transmembrane redox relay associated with parasitism. Plasma membrane PhQ biosynthesis is also predicted to occur in photoautotrophic taxa via alternative splicing, suggesting nonplastidial PhQ is evolutionarily conserved.

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supporting

confidence: 77%

Plasma Membrane Phylloquinone Biosynthesis in Nonphotosynthetic Parasitic Plants

Gu¹,

Chen²,

Nyamdari³

et al. 2018

Preprint

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“…Reverse genetics in parasitic plants is effective only in few chlorophyllous parasitic plants to date (Ishida et al, 2011, 2016; Bandaranayake et al, 2012). These plants are indeed able to develop without a host, which facilitates the production of transgenic lines.…”

Section: Discussionmentioning

confidence: 99%

New Insights into Phloem Unloading and Expression of Sucrose Transporters in Vegetative Sinks of the Parasitic Plant Phelipanche ramosa L. (Pomel)

et al. 2017

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The plant-parasitic plant interaction is a interesting model to study sink-source relationship and phloem unloading. The parasitic plants, such as the achlorophyllous plant Phelipanche ramosa, connect to the host phloem through the haustorium and act as supernumerary sinks for the host-derived photoassimilates, primarily sucrose. The application of the fluorescent symplastic tracer, carboxyfluorescein (CF) derived from carboxyfluorescein diacetate (CFDA), to the leaves of the host plant (Brassica napus) showed direct phloem connections at the host-parasite interface. These experiments also evidenced the dominant apoplastic pathway for phloem unloading in major vegetative sinks of the parasite, including tubercles and shoots, except the adventitious root apices. The CF experiments showed also the symplastic isolation of the phloem tissues from the sink tissues in tubercle and shoot of the parasite, then suggesting the pivotal role of sucrose transporters in sucrose unloading in P. ramosa sinks. Three cDNAs encoding sucrose transporters (PrSUT) were isolated from the parasitic plant. PrSUT1 transcripts accumulated at the same level in the tubercle throughout the parasite growth while a significant increase in transcript accumulation occurred after emergence in the flowering shoot, notably in the growing apical part. The in situ hybridization experiments revealed the PrSUT1 transcript accumulation in the mature phloem cells of both subterranean and flowering shoots, as well as in shoot terminal sinks corresponding to apical meristem, scale leaf primordia and immature vasculature. The transient expression experiments in Arabidopsis protoplasts showed that PrSUT1 was localized at the plasma membrane, suggesting its role in phloem functioning and sucrose uptake by the sink cells in P. ramosa. Conversely, the PrSUT2 transcript accumulation was constantly low in tubercles and shoots but PrSUT3 transcripts accumulated markedly in the subterranean and flowering shoots, in concordance with the PrSUT3 mRNA accumulation in multiple sink areas including apical meristem, scale-leaf primordia, immature vasculature and even storage parenchyma. However, the PrSUT3 transcripts did not accumulate in the mature phloem cells. The transient expression experiments in Arabidopsis protoplasts suggested a tonoplast localization of PrSUT3, for which nevertheless the involvement in intracellular sucrose transport needs clarification.

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“…Similarly, wound-inducible protein kinases that constitute pathogen recognition or calcium signaling are activated within 3 h. It is also interesting to note that genes annotated as responsive to other stresses such as heat, chitin, or biotic stimulus are strongly activated at 1 h after wounding. Previous studies have reported that heat stress induces somatic embryogenesis in some plant species (Fehér 2015), and biotic interactions can trigger cellular proliferation and reprogramming during symbiosis and parasitism (Suzaki et al, 2012;Ishida et al, 2016). It will be thus interesting to test if these stress-induced developmental changes share regulatory mechanisms with wound-induced callus formation.…”

Section: Global Transcriptional Changes During Wound-induced Callus Fmentioning

confidence: 99%

Wounding Triggers Callus Formation via Dynamic Hormonal and Transcriptional Changes

et al. 2017

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Wounding is a primary trigger of organ regeneration, but how wound stress reactivates cell proliferation and promotes cellular reprogramming remains elusive. In this study, we combined transcriptome analysis with quantitative hormonal analysis to investigate how wounding induces callus formation in Arabidopsis (Arabidopsis thaliana). Our time course RNA-seq analysis revealed that wounding induces dynamic transcriptional changes, starting from rapid stress responses followed by the activation of metabolic processes and protein synthesis and subsequent activation of cell cycle regulators. Gene ontology analyses further uncovered that wounding modifies the expression of hormone biosynthesis and response genes, and quantitative analysis of endogenous plant hormones revealed accumulation of cytokinin prior to callus formation. Mutants defective in cytokinin synthesis and signaling display reduced efficiency in callus formation, indicating that de novo synthesis of cytokinin is critical for wound-induced callus formation. We further demonstrate that type-B ARABIDOPSIS RESPONSE REGULATOR-mediated cytokinin signaling regulates the expression of CYCLIN D3;1 (CYCD3;1) and that mutations in CYCD3;1 and its homologs CYCD3;2 and 3 cause defects in callus formation. In addition to these hormone-mediated changes, our transcriptome data uncovered that wounding activates multiple developmental regulators, and we found novel roles of ETHYLENE RESPONSE FACTOR 115 and PLETHORA3 (PLT3), PLT5, and PLT7 in callus generation. All together, these results provide novel mechanistic insights into how wounding reactivates cell proliferation during callus formation.

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Local Auxin Biosynthesis Mediated by a YUCCA Flavin Monooxygenase Regulates Haustorium Development in the Parasitic Plant Phtheirospermum japonicum

Cited by 102 publications

References 84 publications

Plasma Membrane Phylloquinone Biosynthesis in Nonphotosynthetic Parasitic Plants

Plasma Membrane Phylloquinone Biosynthesis in Nonphotosynthetic Parasitic Plants

New Insights into Phloem Unloading and Expression of Sucrose Transporters in Vegetative Sinks of the Parasitic Plant Phelipanche ramosa L. (Pomel)

Wounding Triggers Callus Formation via Dynamic Hormonal and Transcriptional Changes

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