Local antibody response in chickens: analysis of antibody synthesis to Newcastle disease virus by solid-phase radioimmunoassay and immunofluorescence with class-specific antibody for chicken immunoglobulins

Ewert, Donald L.; Barger, Bruce O.; Eidson, C. S.

doi:10.1128/iai.24.1.269-275.1979

Cited by 35 publications

(6 citation statements)

References 25 publications

(26 reference statements)

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“…Previous studies have shown the influx of plasma cells and T cells to the Harderian gland after infection with NDV 5 , 11 , 12 . In the Harderian gland, plasma cells have been shown to synthesize mainly IgA 5 , which has been detected as early as 4 dpi in the saliva of Leghorns 21 after infection with NDV. However, no IgA was detected in the lachrymal fluid of the challenged Fayoumis or Leghorns at 2 & 6 dpi (data not shown).…”

Section: Discussionmentioning

confidence: 99%

Novel analysis of the Harderian gland transcriptome response to Newcastle disease virus in two inbred chicken lines

Deist

Gallardo

Bunn

et al. 2018

Sci Rep

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Behind each eye of the chicken resides a unique lymph tissue, the Harderian gland, for which RNA sequencing (RNA-seq) analysis is novel. We characterized the response of this tissue to Newcastle disease virus (NDV) in two inbred lines with different susceptibility to NDV across three time points. Three-week-old relatively resistant (Fayoumi) and relatively susceptible (Leghorn) birds were inoculated with a high-titered (107EID50) La Sota strain of NDV via an oculonasal route. At 2, 6, and 10 days post infection (dpi) Harderian glands were collected and analyzed via RNA-seq. The Fayoumi had significantly more detectable viral transcripts in the Harderian gland at 2 dpi than the Leghorn, but cleared the virus by 6 dpi. At all three time points, few genes were declared differentially expressed (DE) between the challenged and nonchallenged birds, except for the Leghorns at 6 dpi, and these DE genes were predicted to activate an adaptive immune response. Relative to the Leghorn, the Fayoumi was predicted to activate more immune pathways in both challenged and nonchallenged birds suggesting a more elevated immune system in the Fayoumis under homeostatic conditions. Overall, this study helped characterize the function of this important tissue and its response to NDV.

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Section: Discussionmentioning

confidence: 99%

Novel analysis of the Harderian gland transcriptome response to Newcastle disease virus in two inbred chicken lines

Deist

Gallardo

Bunn

et al. 2018

Sci Rep

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“…The host innate immune system rapidly produces cytokines, chemokines, and interferons in response to the virus, and triggers the adaptive immune response. Antibodies were detected as early as 4 days post infection in the saliva of Leghorns infected with lentogenic NDV [ 19 ]. Also, the lung is home to bronchus-associated lymphoid tissue (BALT), which plays a crucial role in antiviral immunity [ 20 ], and respiratory immune responses are induced in the lung [ 16 ].…”

Section: Introductionmentioning

confidence: 99%

Resistant and susceptible chicken lines show distinctive responses to Newcastle disease virus infection in the lung transcriptome

et al. 2017

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BackgroundNewcastle disease virus (NDV) is a threat to poultry production worldwide. A better understanding of mechanisms of resistance and susceptibility to this virus will improve measures for NDV prevention and control. Males and females from resistant Fayoumi and susceptible Leghorn lines were either challenged with a lentogenic strain of the virus or given a mock infection at 3 weeks of age. The lung transcriptomes generated by RNA-seq were studied using contrasts across the challenged and nonchallenged birds, the two lines, and three time points post-infection, and by using Weighted Gene Co-expression Network Analysis (WGNCA).ResultsGenetic line and sex had a large impact on the lung transcriptome. When contrasting the challenged and nonchallenged birds, few differentially expressed genes (DEG) were identified within each line at 2, 6, and 10 days post infection (dpi), except for the more resistant Fayoumi line at 10 dpi, for which several pathways were activated and inhibited at this time. The interaction of challenge and line at 10 dpi significantly impacted 131 genes (False Discovery Rate (FDR) <0.05), one of which was PPIB. Many DEG were identified between the Fayoumi and Leghorns. The number of DEG between the two lines in the challenged birds decreased over time, but increased over time in the nonchallenged birds. The nonchallenged Fayoumis at 10 dpi showed enrichment of immune type cells when compared to 2 dpi, suggesting important immune related development at this age. These changes between 10 and 2 dpi were not identified in the challenged Fayoumis. The energy allocated to host defense may have interrupted normal lung development. WGCNA identified important modules and driver genes within those modules that were associated with traits of interest, several of which had no known associated function.ConclusionsThe lines’ unique response to NDV offers insights into the potential means of their resistance and susceptibility. The lung transcriptome shows a unique response to lentogenic NDV compared to a previous study on the trachea of the same birds. It is important to analyze multiple tissues in order to best understand the chicken’s overall response to NDV challenge and improve strategies to combat this devastating disease.Electronic supplementary materialThe online version of this article (10.1186/s12864-017-4380-4) contains supplementary material, which is available to authorized users.

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“…Vaccination is the most accepted prevention and control strategy for combating ND in poultry worldwide. Commonly used vaccines are made with low virulence viruses, which provide both cellular and humoral immunity at a low cost, and inactivated oil-emulsion of the same viruses which confer a higher and long lasting humoral immunity [4][5][6]. In addition different recombinant NDV vaccines based on low virulence avian viruses (Herpervirus of Turkey, Fowlpox or low virulence NDV have been developed and used with great success experimentally.…”

Section: Introductionmentioning

confidence: 99%

Development, characterization and optimization of a new suspension chicken-induced pluripotent cell line for the production of Newcastle disease vaccine

Shittu

Zhu

et al. 2016

Biologicals

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Traditionally, substrates for production of viral poultry vaccines have been embryonated eggs or adherent primary cell cultures. The difficulties and cost involved in scaling up these substrates in cases of increased demand have been a limitation for vaccine production. Here, we assess the ability of a newly developed chicken-induced pluripotent cell line, BA3, to support replication and growth of Newcastle disease virus (NDV) LaSota vaccine strain. The characteristics and growth profile of the cells were also investigated. BA3 cells could grow in suspension in different media to a high density of up to 7.0 × 10(6) cells/mL and showed rapid proliferation with doubling time of 21 h. Upon infection, a high virus titer of 1.02 × 10(8) EID50/mL was obtained at 24 h post infection using a multiplicity of infection (MOI) of 5. In addition, the cell line was shown to be free of endogenous and exogenous Avian Leukosis viruses, Reticuloendotheliosis virus, Fowl Adenovirus, Marek's disease virus, and several Mycoplasma species. In conclusion, BA3 cell line is potentially an excellent candidate for vaccine production due to its highly desirable industrially friendly characteristics of growing to high cell density and capability of growth in serum free medium.

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Local antibody response in chickens: analysis of antibody synthesis to Newcastle disease virus by solid-phase radioimmunoassay and immunofluorescence with class-specific antibody for chicken immunoglobulins

Cited by 35 publications

References 25 publications

Novel analysis of the Harderian gland transcriptome response to Newcastle disease virus in two inbred chicken lines

Novel analysis of the Harderian gland transcriptome response to Newcastle disease virus in two inbred chicken lines

Resistant and susceptible chicken lines show distinctive responses to Newcastle disease virus infection in the lung transcriptome

Development, characterization and optimization of a new suspension chicken-induced pluripotent cell line for the production of Newcastle disease vaccine

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