Local and Systemic Immune Mechanisms Underlying the Anti-Colitis Effects of the Dairy Bacterium Lactobacillus delbrueckii

Rocha, Clarissa Santos; Gomes-Santos, Ana Cristina; Moreira, Thaís Garcias; Azevedo, Marli S.P.; Luerce, Tessália Diniz; Mariadassou, Mahendra; Delamare, Ana Paula Longaray; Langella, Philippe; Maguin, Emmanuelle; Azevedo, Vasco; Faria, Ana Maria Caetano; Miyoshi, Anderson; Guchte, Maarten van de

doi:10.1371/journal.pone.0085923

Cited by 48 publications

(45 citation statements)

References 47 publications

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“…L. delbrueckii CNRZ 327 strains were used in this study to show that the pExu vector can replicate in these strains as well as in Top10 and L. lactis . As well documented by Rocha and colleagues, 18 L. delbrueckii subsp. lactis CNRZ 327 has in vitro and in vivo anti-inflammatory activity.…”

Section: Resultssupporting

confidence: 56%

A New Broad Range Plasmid for DNA Delivery in Eukaryotic Cells Using Lactic Acid Bacteria: In Vitro and In Vivo Assays

Mancha-Agresti

Drumond

Carmo

et al. 2017

Molecular Therapy - Methods & Clinical Development

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Lactococcus lactis is well documented as a promising candidate for development of novel oral live vaccines. It has been broadly engineered for heterologous expression, as well as for plasmid expression vector delivery, directly inside eukaryotic cells, for DNA vaccine, or as therapeutic vehicle. This work describes the characteristics of a new plasmid, pExu (extra chromosomal unit), for DNA delivery using L. lactis and evaluates its functionality both by in vitro and in vivo assays. This plasmid exhibits the following features: (1) a theta origin of replication and (2) an expression cassette containing a multiple cloning site and a eukaryotic promoter, the cytomegalovirus (pCMV). The functionality of pExu:egfp was evaluated by fluorescence microscopy. The L. lactis MG1363 (pExu:egfp) strains were administered by gavage to Balb/C mice and the eGFP expression was monitored by fluorescence microscopy. The pExu vector has demonstrated an excellent stability either in L. lactis or in Escherichia coli. The eGFP expression at different times in in vitro assay showed that 15.8% of CHO cells were able to express the protein after transfection. The enterocytes of mice showed the expression of eGFP protein. Thus, L. lactis carrying the pExu is a good candidate to deliver genes into eukaryotic cells.

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Section: Resultssupporting

confidence: 56%

A New Broad Range Plasmid for DNA Delivery in Eukaryotic Cells Using Lactic Acid Bacteria: In Vitro and In Vivo Assays

Mancha-Agresti

Drumond

Carmo

et al. 2017

Molecular Therapy - Methods & Clinical Development

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“…Similar to human IBD, a seemingly paradox increased mucosal infiltration of Foxp3 + T cells is associated with colitis in IL-10R blocked mice [75][76][77]. Since Treg are critical for preventing intestinal inflammation, mucosal Foxp3 + T cells are unable to control gut immune homeostasis in the present study, either by an insufficient amount or cellular dysfunction, as described for immune mediated colitis in human patients [76][77][78][79][80]. The recruitment and sequestration of Foxp3 + T cells in the colon might contribute to the reduced frequency of splenic Treg in IL-10R neutralized mice observed by flow cytometry.…”

Section: Discussionmentioning

confidence: 54%

Viral Infection of the Central Nervous System Exacerbates Interleukin-10 Receptor Deficiency-Mediated Colitis in SJL Mice

Uhde¹,

Herder²,

Khan

et al. 2016

PLoS ONE

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Theiler´s murine encephalomyelitis virus (TMEV)-infection is a widely used animal model for studying demyelinating disorders, including multiple sclerosis (MS). The immunosuppressive cytokine Interleukin (IL)-10 counteracts hyperactive immune responses and critically controls immune homeostasis in infectious and autoimmune disorders. In order to investigate the effect of signaling via Interleukin-10 receptor (IL-10R) in infectious neurological diseases, TMEV-infected SJL mice were treated with IL-10R blocking antibody (Ab) in the acute and chronic phase of the disease. The findings demonstrate that (i) Ab-mediated IL-10 neutralization leads to progressive colitis with a reduction in Foxp3+ regulatory T cells and increased numbers of CD8+CD44+ memory T cells as well as activated CD4+CD69+ and CD8+CD69+ T cells in uninfected mice. (ii) Concurrent acute TMEV-infection worsened enteric disease-mediated by IL-10R neutralization. Virus-triggered effects were associated with an enhanced activation of CD4+ T helper cells and CD8+ cytotoxic T lymphocytes and augmented cytokine expression. By contrast, (iii) IL-10R neutralization during chronic TMEV-infection was not associated with enhanced peripheral immunopathology but an increased CD3+ T cell influx in the spinal cord. IL-10R neutralization causes a breakdown in peripheral immune tolerance in genetically predisposed mice, which leads to immune-mediated colitis, resembling inflammatory bowel disease. Hyperactive immune state following IL-10R blockade is enhanced by central nervous system-restricted viral infection in a disease phase-dependent manner.

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“…Similar properties were reported for lactic acid bacteria, which may be used for Emmental cheese manufacturing [22]. Regarding all these data, we hypothesized that selection of specific lactic acid bacteria and dairy propionibacteria strains presenting anti-inflammatory properties could lead to a potentially probiotic Emmental favouring the treatment of IBD [19,20,23,24]. Therefore, the aim of this study was to evaluate the beneficial impact of an Emmental, made using three selected anti-inflammatory strains P. freudenreichii CIRM-BIA 129 [20], Lactobacillus delbrueckii subsp.…”

Section: Introductionmentioning

confidence: 53%

“…Therefore, the aim of this study was to evaluate the beneficial impact of an Emmental, made using three selected anti-inflammatory strains P. freudenreichii CIRM-BIA 129 [20], Lactobacillus delbrueckii subsp. lactis CNRZ327 [23], and Streptococcus thermophilus LMD-9 [25], in the context of Dextran Sodium Sulphate (DSS)-induced colitis in mice.…”

Section: Introductionmentioning

confidence: 99%

Beneficial Propionibacteria within a Probiotic Emmental Cheese: Impact on Dextran Sodium Sulphate-Induced Colitis in Mice

et al. 2020

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Backgrounds and Aims. Inflammatory Bowel Diseases (IBD), including Ulcerative Colitis (UC), coincide with alterations in the gut microbiota. Consumption of immunomodulatory strains of probiotic bacteria may induce or prolong remission in UC patients. Fermented foods, including cheeses, constitute major vectors for bacteria consumption. New evidences revealed anti-inflammatory effects in selected strains of Propionibacterium freudenreichii. We thus hypothesized that consumption of a functional cheese, fermented by such a strain, may exert a positive effect on IBD. Methods. We investigated the impact of cheese fermented by P. freudenreichii on gut inflammation. We developed an experimental single-strain cheese solely fermented by a selected immunomodulatory strain of P. freudenreichii, CIRM-BIA 129. We moreover produced, in industrial conditions, an Emmental cheese using the same strain, in combination with Lactobacillus delbrueckii CNRZ327 and Streptococcus thermophilus LMD-9, as starters. Consumption of both cheeses was investigated with respect to prevention of Dextran Sodium Sulphate (DSS)-induced colitis in mice. Results. Consumption of the single-strain experimental cheese, or of the industrial Emmental, both fermented by P. freudenreichii CIRM-BIA 129, reduced severity of subsequent DSS-induced colitis, weight loss, disease activity index and histological score. Both treatments, in a preventive way, reduced small bowel Immunoglobulin A (IgA) secretion, restored occludin gene expression and prevented induction of Tumor Necrosis Factor α (TNFα), Interferon γ (IFNγ) and Interleukin-17 (IL-17). Conclusions. A combination of immunomodulatory strains of starter bacteria can be used to manufacture an anti-inflammatory cheese, as revealed in an animal model of colitis. This opens new perspectives for personalised nutrition in the context of IBD.

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Local and Systemic Immune Mechanisms Underlying the Anti-Colitis Effects of the Dairy Bacterium Lactobacillus delbrueckii

Cited by 48 publications

References 47 publications

A New Broad Range Plasmid for DNA Delivery in Eukaryotic Cells Using Lactic Acid Bacteria: In Vitro and In Vivo Assays

A New Broad Range Plasmid for DNA Delivery in Eukaryotic Cells Using Lactic Acid Bacteria: In Vitro and In Vivo Assays

Viral Infection of the Central Nervous System Exacerbates Interleukin-10 Receptor Deficiency-Mediated Colitis in SJL Mice

Beneficial Propionibacteria within a Probiotic Emmental Cheese: Impact on Dextran Sodium Sulphate-Induced Colitis in Mice

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