LOC285629 regulates cell proliferation and motility in colorectal cancer cells

Nasir, Siti Nurmi; Abu, Nadiah; Mutalib, Nurul Syakima Ab; Ishak, Muhiddin; Sagap, Ismail; Mazlan, Luqman; Röse, I; Jamal, Rahman

doi:10.1007/s12094-017-1788-x

Cited by 4 publications

(3 citation statements)

References 31 publications

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“…Moreover, ENO2 was found to be significantly upregulated in a metastatic colon cancer cell line, which indicated a likely correlation with the metastatic mechanism in vitro and in vivo [ 28 ]. Some studies showed that a lncRNA (LOC285629) is involved in CRC pathogenesis through direct or indirect association with ENO2 [ 29 ]. Other research indicated that ENO2 combined with other known CRC markers can distinguish early-phase malignant colorectal tumors from benign tumors [ 30 ].…”

Section: Discussionmentioning

confidence: 99%

Prognostic Value of Enolase Gene Family in Colon Cancer

Xiao-hang

Chen

et al. 2020

Med Sci Monit

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Background Colorectal cancer (CRC), the most common gastrointestinal cancer, is associated with high mortality rates. Enolase is a major enzyme present in the glycolytic pathway. However, the functional significance of the enolase (ENO) gene family in the pathogenesis of CRC has been unclear. Material/Methods The data associated with 438 CRC patients from The Cancer Genome Atlas database were extracted for analysis. Survival analyses with Cox regression was performed to construct a prognostic signature. We investigated the processes that underlies the correlation between ENO genes and overall survival (OS) using gene set enrichment analysis (GSEA). We then developed a connectivity map to identify candidate target drugs for CRC. Results The multivariate survival analysis showed that low expression of ENO2 and ENO3 had a significant correlation with longer OS. The joint-effects survival analysis indicated that the combined low expression of ENO2 and ENO3 was highly correlated with favorable OS. As indicated by the gene set enrichment analysis (GSEA), the ENO gene is involved in various biological pathways and has multiple roles. Potential pharmacological targets of ENO2 and ENO3 were constructed as well. Conclusions Low expression levels of both ENO2 and ENO3 were linked to a positive prognosis for CRC. Both ENO2 and ENO3 show promise as prognostic biomarkers for colon cancer patients.

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Section: Discussionmentioning

confidence: 99%

Prognostic Value of Enolase Gene Family in Colon Cancer

Xiao-hang

Chen

et al. 2020

Med Sci Monit

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“…本研究通过对TCGA数据库样本的整理和铜死亡相关lncRNA的筛选，利用多因素Cox回归分析共获得9个与膀胱癌患者预后相关的铜死亡相关lncRNA，其中 AC105001.1 、 AC103746.1 、 AL031651.2 和 LINC02159 为膀胱癌患者预后的危险因素， AC108516.2 、 AC007993.3 、 LINC01648 、 AC006160.1 和 SCAMP1 - AS1 为保护因素。有研究显示，在发生心肌梗死的患者中， AC103746.1 在血管生成中具有重要的促进作用［ 20 ］。膀胱癌是公认的具有高度血管化的肿瘤，血管密度作为膀胱癌患者预后的独立指标，同时高血管密度也是癌症复发的危险因素［ 21 - 22 ］。本文资料提示 AC103746.1 是膀胱癌患者预后的不良因素，推测可能是通过影响血管形成从而促进膀胱癌的进展。 LINC02159 ，又名 LOC285629 ，为食管鳞癌的低风险基因之一［ 23 ］，可能通过降低PGRN、PDGF-AA、烯醇化酶2和p70核糖体蛋白S6激酶等表达从而阻碍结直肠癌进展，并且可能降低癌症的耐药性，其表达水平与结直肠癌预后显著相关［ 24 ］。但膀胱癌中PGRN/EphA2轴可能会促进血管形成，有助于膀胱癌的进展［ 25 ］。因此， LINC02159 可能通过靶向PGRN促进膀胱癌的发生和发展。在保护因素方面，有研究显示 AC006160.1 过表达可显著抑制膀胱癌细胞株的增殖和侵袭能力，可作为膀胱癌发展的保护因素［ 26 ］，本研究结果与之相符。在肺腺癌患者中， SCAMP1 - AS1 在N2期中的表达低于N0分期和N1分期［ 27 ］，表明 SCAMP1 - AS1 可能在肺腺癌中抑制肿瘤进展，本文资料显示 SCAMP1 - AS1 在膀胱癌中同样扮演阻碍其发生和进展的角色。除上述4个lncRNA外，有文献报道 LINC01648 与糖化血红蛋白相关［ 28 ］，其余4个lncRNA的功能则尚未见文献报道，需要进一步研究。…”

Section: 讨论unclassified

基于铜死亡相关的 lncRNA的膀胱癌预后模型的构建及免疫浸润分析

Xu¹,

Chen²,

Cui³

et al. 2023

J Zhejiang Univ (Med Sci)

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目的基于铜死亡相关长链非编码RNA（lncRNA）构建膀胱癌患者预后风险评估模型。方法下载癌症基因组图谱数据库中的膀胱癌患者RNA序列数据和临床数据，采用Pearson相关性分析、单因素Cox回归、Lasso回归和多因素Cox回归分析筛选与铜死亡及膀胱癌患者预后相关的lncRNA，并构建铜死亡相关的lncRNA膀胱癌患者预后风险评分方程。根据风险评分方程计算的中位数将患者分为高风险组和低风险组，比较两组免疫细胞丰度差异。应用Kaplan-Meier生存曲线评估风险评分方程的准确性；应用受试者操作特征曲线（ROC曲线）评估风险评分方程预测患者1、3、5年存活率的价值；采用单因素和多因素Cox回归筛选与膀胱癌患者预后相关的影响因素，构建膀胱癌患者预后风险评估列线图，并通过校准曲线评估列线图预测的准确性。结果膀胱癌患者预后风险评分方程由9个铜死亡相关的lncRNA构建。免疫浸润分析结果显示，高风险组M0巨噬细胞、M1巨噬细胞、M2巨噬细胞、静息肥大细胞及中性粒细胞丰度明显高于低风险组，而低风险组CD8 + T细胞、辅助性T细胞、调节性T细胞及浆细胞丰度明显高于高风险组（均 P <0.05）。Kaplan-Meier生存曲线分析结果显示，与高风险组比较，低风险组的总生存期和无进展生存期更长（均 P <0.01）。单因素和多因素Cox回归分析结果显示，风险评分、年龄及肿瘤分期为患者预后的独立影响因素。ROC曲线分析结果显示，风险评分预测患者1、3、5年生存曲线下面积（AUC）分别为0.716、0.697、0.717，当联合年龄、肿瘤分期后，其预测患者1年生存的AUC可提高至0.725。基于患者年龄、肿瘤分期和风险评分构建的膀胱癌患者预后风险评估列线图，其预测值与实际值基本一致。结论基于铜死亡相关lncRNA构建的膀胱癌患者预后风险评估模型不仅能较准确地预测膀胱癌患者的预后，还可以评估患者的免疫浸润状态，为后续肿瘤免疫治疗提供参考。

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“…Tumor and non-tumor tissue samples were obtained from CRC patients during surgical resection of the tumor. A subset of tissues from these patients was previously used to detect a specific long non-coding RNA in a different study [21] . Demographic data of the patients is shown in Table 1.…”

Section: Collection Of the Tissue Specimensmentioning

confidence: 99%

Circular RNA-EPHB4 As A Potential Biotarget In Colorectal Cancer: A Preliminary Analysis

Nasir¹,

Ishak²,

Mutalib³

et al. 2022

Prog Micobes Mol Biol

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Colorectal cancer (CRC) is among the top causes of cancer-related deaths. With the advent of new biomedical technologies, new therapeutic targets are being actively discovered. Circular RNAs (circRNAs) are characterized by the absence of covalently closed-loop structures at the 3′ and 5′ ends. Recent studies have shown that circRNAs are abnormally expressed in tumor tissues. Certain circRNAs have been reported to have potential as cancer biomarkers. Among all the circRNAs previously discovered, circ-EPHB4 seemed promising. Therefore, this study is aimed to determine the function of circEPHB4 in CRC. By utilizing in-silico prediction tools, qPCR and cell-based assays, we were able to provide preliminary insights into the role of circEPHB4. The expression of both circEPHB4 and linear EPHB4 were upregulated in our Malaysian CRC tissues and commercial cell lines as compared to the non-cancerous counterparts. Reduction of both circEPHB4 and EPHB4 had effects on wound healing, drug response and clonogenic abilities of SW-480 and HCT-116 cells. Our initial findings suggest that circ-EPHB4 may be involved in the regulation of CRC though further in-depth studies are needed.

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LOC285629 regulates cell proliferation and motility in colorectal cancer cells

Abstract: Our findings suggest that LOC285629 may be further developed as a potential therapeutic target for CRC treatment.

Cited by 4 publications

References 31 publications

Prognostic Value of Enolase Gene Family in Colon Cancer

Prognostic Value of Enolase Gene Family in Colon Cancer

基于铜死亡相关的 lncRNA的膀胱癌预后模型的构建及免疫浸润分析

Circular RNA-EPHB4 As A Potential Biotarget In Colorectal Cancer: A Preliminary Analysis

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